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- Support & Troubleshoot[92]
pSilencer™ 2.1-U6 Puro
| Catalog # | Unit Size | List Price | |
|---|---|---|---|
| AM5762 | 20 reactions | 398.00 |
Description
This Ambion® vector is for the expression of siRNA. It has an antibiotic resistance gene (puromycin) to enable selection of transfected cells and features the human RNA View full product details >
General Specifications
- Promoter
-
U6
- RNAi Type
-
shRNA
- Structure
-
Linear
- Vector Type
-
pSilencer
- Product Size
-
20 reactions
- Cloning Method
-
Restriction Enzyme ⁄ MCS
- Delivery Method
-
Transfection
- Selection Marker Promoter
-
SV40 Promoter
- Selection Agent (Eukaryotic)
-
Puromycin
- Constitutive or Inducible System
-
Constitutive
- Shipping Condition
-
Dry Ice
- Regulatory Statement
-
For Research Use Only. Not for use in diagnostic procedures.
Product Applications
- RNAi, Epigenetics & Non-Coding RNA Research
User Ratings
Rate this productpSilencer™ 2.1-U6 Neo
| Catalog # | Unit Size | List Price | |
|---|---|---|---|
| AM5764 | 20 reactions | 398.00 |
Description
This Ambion® vector is for the expression of siRNA. It has an antibiotic resistance gene (neomycin) to enable selection of transfected cells and features the human RNA View full product details >
General Specifications
- Promoter
-
U6
- RNAi Type
-
shRNA
- Structure
-
Linear
- Vector Type
-
pSilencer
- Product Size
-
20 reactions
- Cloning Method
-
Restriction Enzyme ⁄ MCS
- Delivery Method
-
Transfection
- Selection Marker Promoter
-
SV40 Promoter
- Selection Agent (Eukaryotic)
-
Geneticin® (G-418)
- Selection Marker (Eukaryotic)
-
NeoR
- Constitutive or Inducible System
-
Constitutive
- Shipping Condition
-
Dry Ice
- Regulatory Statement
-
For Research Use Only. Not for use in diagnostic procedures.
Product Applications
- RNAi, Epigenetics & Non-Coding RNA Research
User Ratings
pSilencer™ 2.1-U6 Neo with Manual
| Catalog # | Unit Size | List Price | |
|---|---|---|---|
| AM5764M | 20 reactions w⁄ Manual | 354.00 |
Description
This Ambion® vector is for the expression of siRNA. It has an antibiotic resistance gene (neomycin) to enable selection of transfected cells and features the human RNA View full product details >
General Specifications
- Promoter
-
U6
- RNAi Type
-
shRNA
- Structure
-
Linear
- Vector Type
-
pSilencer
- Product Size
-
20 reactions w⁄ Manual
- Cloning Method
-
Restriction Enzyme ⁄ MCS
- Delivery Method
-
Transfection
- Selection Agent (Eukaryotic)
-
Geneticin® (G-418)
- Constitutive or Inducible System
-
Constitutive
- Regulatory Statement
-
For Research Use Only. Not for use in diagnostic procedures.
Product Applications
- RNAi, Epigenetics & Non-Coding RNA Research
User Ratings
pSilencer™ 2.1-U6 Puro with Manual
| Catalog # | Unit Size | List Price | |
|---|---|---|---|
| AM5762M | 20 reactions w⁄ Manual | 354.00 |
Description
This Ambion® vector is for the expression of siRNA. It has an antibiotic resistance gene (puromycin) to enable selection of transfected cells and features the human RNA View full product details >
General Specifications
- Promoter
-
U6
- RNAi Type
-
shRNA
- Structure
-
Linear
- Vector Type
-
pSilencer
- Product Size
-
20 reactions w⁄ Manual
- Cloning Method
-
Restriction Enzyme ⁄ MCS
- Delivery Method
-
Transfection
- Selection Agent (Eukaryotic)
-
Puromycin
- Constitutive or Inducible System
-
Constitutive
- Regulatory Statement
-
For Research Use Only. Not for use in diagnostic procedures.
Product Applications
- RNAi, Epigenetics & Non-Coding RNA Research
User Ratings
pSilencer™ 2.1-U6 Hygro with Manual
| Catalog # | Unit Size | List Price | |
|---|---|---|---|
| AM5760M | 1 kit w⁄ Manual | 398.00 |
Description
This Ambion® vector is for the expression of siRNA. It has an antibiotic resistance gene (hygromycin) to enable selection of transfected cells and features the human RNA View full product details >
General Specifications
- Promoter
-
U6
- RNAi Type
-
shRNA
- Structure
-
Linear
- Vector Type
-
pSilencer
- Product Size
-
1 kit w⁄ Manual
- Cloning Method
-
Restriction Enzyme ⁄ MCS
- Delivery Method
-
Transfection
- Selection Agent (Eukaryotic)
-
Hygromycin
- Constitutive or Inducible System
-
Constitutive
- Regulatory Statement
-
For Research Use Only. Not for use in diagnostic procedures.
Product Applications
- RNAi, Epigenetics & Non-Coding RNA Research
User Ratings
pSilencer™ 2.1-U6 Hygro
| Catalog # | Unit Size | List Price | |
|---|---|---|---|
| AM5760 | 20 reactions | 398.00 |
Description
This Ambion® vector is for the expression of siRNA. It has an antibiotic resistance gene (hygromycin) to enable selection of transfected cells and features the human RNA View full product details >
General Specifications
- Promoter
-
U6
- RNAi Type
-
shRNA
- Structure
-
Linear
- Vector Type
-
pSilencer
- Product Size
-
20 reactions
- Cloning Method
-
Restriction Enzyme ⁄ MCS
- Delivery Method
-
Transfection
- Selection Marker Promoter
-
SV40 Promoter
- Selection Agent (Eukaryotic)
-
Hygromycin
- Constitutive or Inducible System
-
Constitutive
- Shipping Condition
-
Dry Ice
- Regulatory Statement
-
For Research Use Only. Not for use in diagnostic procedures.
Product Applications
- RNAi, Epigenetics & Non-Coding RNA Research
User Ratings
Cloning siRNAs into pSilencer | Life Technologies
Experiment Protocol
Cloning siRNAs into pSilencer
Overview
Since the launch of our pSilencer family of vectors, many researchers have asked us which competent cells are recommended for cloning small hairpin siRNA-encoding
pSilencer Vectors | Life Technologies
Experiment Protocol
pSilencer Vectors
Overview
The first step in preparing a plasmid for siRNA experiments is to identify target sequences in the gene of interest that are susceptible to siRNA-induced degradation.
pSilencer™ 5.1 Retro Instruction Manual
Manual / Product Insert
Protocol: pSilencer 5.1 Retro Kit
Manual / Product Insert
pSilencer™ hygro Instruction Manual
Manual / Product Insert
pSilencer™ neo Instruction Manual
Manual / Product Insert
