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Optimized RT-PCR with Invitrogen™ Cloned AMV Reverse Transcriptase
Citations & References
- Authors: Amy Wayland-Smith, Gulshan Dhariwal, Michael D. Smith, R. Ayoub Rashtchian, Gary F. Gerard, Jason Potter, Jun E. Lee
- Journal: Focus (2002) 24:14-15
- Product Usage: Using Cloned AMV RT under optimized reaction conditions in RT-PCR applications.
- ID: PN65719
140f6969d5213fd0ece03148e62e461e_Citations
Inhibition of AMV DNA polymerase by polyriboadenylic acid containing epsilon-adenosine residues.
Citations & References
- Authors: Chirikjian JG, Papas TS
- Journal: Biochem Biophys Res Commun (1974) 59:489-495
- Product Usage:
- ID: PN1361
- Catalog #s
- E1020
7b18ceae096a51a11c3f945abece6238_Citations
Chloride-dependent Na-H exchange. A novel mechanism of sodium transport in colonic crypts.
Citations & References
- Authors: Rajendran VM, Geibel J, Binder HJ
- Journal: J Biol Chem (1995) 270:11051-11054
- Product Usage:
- ID: PN19781
fd233385e60c83bac665ba45233dadae_Citations
The role of template-primer in protection of reverse transcriptase from thermal inactivation.
Citations & References
- Authors: Gerard GF, Potter RJ, Smith MD, Rosenthal K, Dhariwal G, Lee J, Chatterjee DK,
- Journal: Nucleic Acids Res (2002) 30:3118-3129
- Product Usage: Comparison of thermal stabilities of wild-type recombinant avian myeloblastosis virus (AMV) and Moloney murine leukemia virus (M-MLV) reverse transcriptase (RT) with those of mutants of the recombinant enzymes lacking RNase H activity.
- ID: PN65017
c2c1fc225872c8f4b8b18410e10ff072_Citations
Mechanism of c-Myb-C/EBPbeta Cooperation from Separated Sites on a Promoter.
Citations & References
- Authors: Tahirov Tahir H; Sato Ko; Ichikawa-Iwata Emi; Sasaki Motoko; Inoue-Bungo Taiko; Shiina Masaaki; Kimura Kazumi; Takata Shioka; Fujikawa Atsushi; Morii Hisayuki; Kumasaka Takashi; Yamamoto Masaki; Ishii Shunsuke; Ogata Kazuhiro;
- Journal: Cell (2002) 108:57-70
- Product Usage: QT6 cells (a quail fibroblast cell line) were cultured in Dulbecco's modified Eagle medium (DMEM) supplemented with 5% fetal calf serum, 2% chicken serum, and 2% triptose phosphate broth under a 5% CO2 atmosphere at 37°C. DNA transfection of the cells was carried out using Lipofectamine 2000 according to the manufacturer's instructions.
- ID: PN62372
- Catalog #s
- 11668019
db6d9e1beb13d90e4a67706afb39e4e8_Citations
High processivity of the reverse transcriptase from a non-long terminal repeat retrotransposon.
Citations & References
- Authors: Bibillo A, Eickbush TH
- Journal: J Biol Chem (2002) 277:34836-34845
- Product Usage:
- ID: PN55354
e9277b4c7ff99a69b9cc2a7083543612_Citations
Reverse transcriptase inhibits Taq polymerase activity.
Citations & References
- Authors: Sellner LN; Coelen RJ; Mackenzie JS
- Journal: Nucleic Acids Research 1992 7:1487-1490
- Product Usage: AmpliTaq® DNA Polymerase
- ID: PN92020
964950f58404a40a5c7913eb6bc9c260_Citations
Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning.
Citations & References
- Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I
- Journal: Anal Biochem (1998) 256:82-91
- Product Usage:
- ID: PN29528
a4ad4c62b6a12892242bd6786d83f9aa_Citations
Comparison of three RNA amplification methods as sources of DNA for sequencing.
Citations & References
- Authors: Chadwick N, Wakefield AJ, Pounder RE, Bruce IJ
- Journal: Biotechniques (1998) 25:818-822
- Product Usage: The error rates of several cDNA generation systems (RT-PCR) were compared. The measles virus genome was reverse transcribed and amplified using the Copy kit and other methods and the number of mutations that were generated were compared after sequencing.
- ID: PN61139
1b26393c0dbab1e5a039ae09c001d907_Citations
Template properties of mutagenic cytosine analogues in reverse transcription.
Citations & References
- Authors: Suzuki T; Moriyama K; Otsuka C; Loakes D; Negishi K
- Journal: Nucleic Acids Research 2007 22:6438-6449
- Product Usage: Capillary Electrophoresis Systems
- ID: PN72769
7ee0012f0b3500e3b914920486605533_Citations
Novel fluorogenic substrates for assaying retroviral proteases by resonance energy transfer.
Citations & References
- Authors: Matayoshi ED, Wang GT, Krafft GA, Erickson J
- Journal: Science (1990) 247:954-958
- Product Usage:
- ID: PN12625
- Catalog #s
- H2930
babbaf1148b4298e19dd5d3dd251cdce_Citations
A Mammalian H+ Channel Generated Through Alternative Splicing of the NADPH Oxidase Homolog NOH-1
Citations & References
- Authors: Banfi B, Maturana A, Jaconi S, Arnaudeau S, Laforge T, Sinha B, Ligeti E, Demaurex N, Krause KH
- Journal: Science (2000) 287:138-142
- Product Usage: Polyadenylated RNA was purified with the Fast Track kit from CaCo-2, dimethyl sulfoxide DMSO)-differentiated HL-60 cells, human peripheral blood leukocytes, and HEK-293 cells. First-strand cDNA was generated using AMV reverse transcriptase with random primers. The entire coding regions of NOH-1S and NOH-1L were amplified and subcloned into pcDNA3.1 expression vector and used to stably transfect HEK-293 cells by the calcium phosphate coprecipitation method. Ten Ág of total RNA from tissues or 5
- ID: PN62500
3f13cf4ddf6fc50c0d39a1d5aeb57dd8_Citations
Dual receptor T cells extend the immune repertoire for foreign antigens.
Citations & References
- Authors: He Xin; Janeway Charles A Jr; Levine Matthew; Robinson Eve; Preston-Hurlburt Paula; Viret Christophe; Bottomly Kim;
- Journal: Nat Immunol (2002) 3:127-161
- Product Usage: RNA was extracted with the TRIzol reagent and cDNA was synthesized with the AMV reverse transcriptase and an oligo(dT) primer. Genomic DNA was extracted with the DNAzol kit. The secondary PCR products were gel-purified again and cloned into the PCR2.1 cloning-vector with the TA cloning kit.
- ID: PN62728
- Catalog #s
- 15596026
39d50f6c9228b8f3c8830c18b8f91ab2_Citations
The blood group P1 synthase gene is identical to the Gb3/CD77 synthase gene. A clue to the solution of the P1/P2/p puzzle.
Citations & References
- Authors: Iwamura K, Furukawa K, Uchikawa M, Sojka BN, Kojima Y, Wiels J, Shiku H, Urano T, Furukawa K,
- Journal: J Biol Chem (2003) 278:44429-44438
- Product Usage: Total RNA was isolated using Trizol from human red blood cells (1 ml) prepared from healthy donors with their consent. cDNA was synthesized by AMV from 3 µg of total RNA as a template. Luciferase expression vectors were cotransfected with pRL-TK vector for normalization of transfection efficiency into NCC-IT cells using LipofectAMINE. After 48 h of transfection, the medium was removed, the cells were washed twice with PBS, and lysates were prepared to independently measure luciferase activity.
- ID: PN63004
ec4a427258aa615cc0b56df3b79683ac_Citations
Evolution of moth sex pheromones via ancestral genes.
Citations & References
- Authors: Roelofs Wendell L; Liu Weitian; Hao Guixia; Jiao Hongmei; Rooney Alejandro P; Linn Charles E Jr;
- Journal: Proc Natl Acad Sci U S A (2002) 99:13621-13627
- Product Usage: Poly(A)+ RNA from ACB was dephosphorylated and decapped with a GeneRacer kit. The decapped mRNA was ligated with GeneRacer RNA Oligo and reverse-transcribed with avian myeloblastosis virus (AMV) reverse transcriptase and GeneRacer Oligo(dT) Primer. The ORF of ECB-PG3 was cloned to a pFastBac1 vector which was then transposed to the baculovirus genome (bacmid DNA) by using a baculovirus expression system.
- ID: PN65227
d33c10c1a53c1e860a631c00f02d748a_Citations
