Eliminate the safety concerns of UV transillumination

• Does not damage your skin and eyes
• Produces brighter light and more uniform emission than conventional blue light sources
• Provides optimal excitation for SYBR® Safe DNA gel stain
• Optimized for use with other nucleic acid and protein stains such SYBR® Gold, SYBR® Green I & II, SYPRO® Ruby, SYPRO® Orange, and Coomassie Fluor™ Orange stains

Instrument Specifications

• Overall dimensions: 28 × 31 × 7 cm (11 × 12.25 × 2.75 in)
• Viewing surface dimensions: 20 × 20 cm (7.87 × 7.87 in)
• Light source: light emitting diodes (LED) producing a narrow emission peak centered at ~470 nm
• LED life: 100,000 hours
• Included accessories: amber filter unit, viewing glasses, and international power cord

SYBR® Safe DNA gel stain

The safer DNA gel stain

• Displays no acute oral toxicity and greatly reduced genotoxicity
• Offers non-hazardous waste status and Clean Water Act compliance
• Reduces waste storage and disposal costs
• Provides uncompromised detection sensitivity as compared to ethidium bromide

Gels loaded with equal amounts of a PCR product (1.25 kb gene fragment from Ultimate™ ORF IOH# 11050) were stained with either SYBR® Safe DNA gel stain (1:10,000 in TBE) or ethidium bromide (0.5 µg/ml in TBE) following electrophoresis. The gel stained with SYBR® Safe stain was visualized on a blue-light box with light emission identical to that produced by the Safe Imager™ transilluminator (S37102). The ethidium bromide-stained gel was visualized using UV transillumination. Bands were excised at defined exposure times. DNA was purified from the gel fragments under identical conditions and used in parallel sub-cloning reactions. Following transformation into OneShot® TOP 10 chemically competent bacteria, three serial dilutions were plated and colonies counted using an Alpha Innotech imaging system. A plot of the experiment is shown here.