D-LUX™ Gene Expression Assays
D-LUX™ Gene Expression Assays use the latest generation of LUX™ (Light Upon eXtension) qPCR detection technology that combines robust chemistry with optimized bioinformatics to deliver the most sensitive, specific, and cost-effective detection method available for qPCR/qRT-PCR. With the D-LUX™ Detection Platform, you can:
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How D-LUX™ Detection Technology Works
D-LUX™ detection technology uses one primer labeled with a single fluorophore and a corresponding unlabeled primer, both custom-synthesized according to the target of interest. Typically 20-30 bases in length, LUX™ Primers are designed with a fluorophore near the 3´end in a hairpin structure. This configuration intrinsically renders fluorescence quenching capability, making a separate quenching moiety unnecessary. When the primer becomes incorporated into the double-stranded PCR product, the fluorophore is de-quenched, resulting in a significant increase in fluorescent signal (Figure 1).
D-LUX™ detection technology uses one primer labeled with a single fluorophore and a corresponding unlabeled primer, both custom-synthesized according to the target of interest. Typically 20-30 bases in length, LUX™ Primers are designed with a fluorophore near the 3´end in a hairpin structure. This configuration intrinsically renders fluorescence quenching capability, making a separate quenching moiety unnecessary. When the primer becomes incorporated into the double-stranded PCR product, the fluorophore is de-quenched, resulting in a significant increase in fluorescent signal (Figure 1).
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| Assay Design | Assay Validation | Species | Dye Labels | |
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| Certified LUX™ Assays | Pre-Designed | Functional | Infectious Diseases | FAM |
| Reference Genes: multiple species | FAM, JOE | |||
| D-LUX™ Select Gene Expression Assays | Pre-Designed | In silico | Human, Mouse, Rat | FAM, JOE, Alexa Fluor 546 |
| Custom D-LUX™ Assays | Customer Designed Online | Any | FAM, JOE, Alexa Fluor 546 | |
| D-LUX™ Design and Validation Service | Designed by Invitrogen Scientists | Functional | Any | FAM, JOE, Alexa Fluor 546 |
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Figure 1—The LUX™ (Light Upon eXtension) effect
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Combine the best of probe-based and SYBR® Green detection
The D-LUX™ Detection Platform offers the sensitivity, specificity and dynamic range of TaqMan® probes, while providing the ease-of-use, cost-effectiveness and melting curve analysis capability of SYBR Green (Table 1).
Table 1—Comparison of real-time detection platforms
The D-LUX™ Detection Platform offers the sensitivity, specificity and dynamic range of TaqMan® probes, while providing the ease-of-use, cost-effectiveness and melting curve analysis capability of SYBR Green (Table 1).
Table 1—Comparison of real-time detection platforms
| TaqMan® Probes | Molecular Beacons | SYBR® Green 1 | LUX™ Primers | |
|---|---|---|---|---|
| Sensitivity | ••• | ••• | • | ••• |
| Dynamic range | ••• | ••• | • | ••• |
| Specificity | ••• | ••• | • | ••• |
| Multiplexing | •• | •• | N/A | ••• |
| Melting curve analysis | N/A | N/A | ••• | ••• |
| Ease of design | • | • | ••• | ••• |
| Cost | • | • | ••• | ••• |
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