Yeast and Cellmask and Plasma Membrane
The CellMask™ Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. These stains may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for standard fluorescence microscopy. The stain survives fixation, but not permeabilization, so it is not suitable for experiments that also involve probing internal targets via antibodies.
Catalog: C10046
Size: 100 µl
Size: 100 µl
PVDF Pre-Cut Blotting Membranes (0.2 µm pore size) are membrane/filter paper sandwiches pre-cut and pre-assembled—ready for protein blotting/transfer. Twenty membrane/filter paper sandwiches are provided, for 20 transfers. Applications Western transfer, protein sequencing, amino acid analysis, solid phase assay systems Dimensions 8.3 cm x 7.3 cm Binding capacity 50–150 µg/cm 2 for large, globular proteins; >150 µg/cm 2 for smaller peptides A protein's properties (i.e., charge, hydrophobicity, etc.) affect its ability to bind to membrane surfaces. Finding the right membrane may require experime...
Catalog: LC2002
Size: 20⁄pack
Size: 20⁄pack
ARCTURUS® laser capture microdissection (LCM) instruments offer a unique combination of infrared (IR) laser capture and ultraviolet (UV) laser cutting, permitting the use of any slide type and any sample preparation for microdissection. While plain glass slides are effectively used for standard IR-based LCM, polyethylene naphthalate (PEN) membrane slides facilitate this Arcturus®-exclusive dual laser function and allow you to determine the appropriate sample preparation based on your application need. Plain Glass Slides The ARCTURUS® instruments are the only LCM systems that allow efficient us...
Catalog: LCM0522
Size: 50 each
Size: 50 each
Yeast tRNA is suitable for use as a carrier in nucleic acid purification and precipitation procedures. It is supplied in lyophilized form.
Catalog: 15401-011
Size: 25 mg
Size: 25 mg
This concentrated Nitro-Block-II™ Enhancer can be used with CSPD® or CDP-Star® chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II™ membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF membranes. • Chemically optimized formulations provide enhanced signal intensity in blotting applications with dioxetane-based substrate solutions (CSPD® or CDP-Star® substrates) on nitrocellulose or PVDF membranes. • Enhancer increases signal intensity, allowing decreased signal acquisition time. Increa...
Catalog: T2184
Size: 20 ml
Size: 20 ml
HCS CellMask™ Red stain (H32712) replaces the HCS CellMask™ Red cytoplasmic⁄nuclear stain (H32711) as a cell delineation tool for HCS platforms. HCS CellMask™ stains label the entire cell (cytoplasm and nucleus) and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask™ stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If only nuclear staining is desired, HCS NuclearMask™ stains can be used for measuring DNA content in ...
Catalog: H32712
Size: 1 set
Size: 1 set
A suite of accessories has been designed to enable research on living cells using your ArcturusXT™ LCM Instrument. Researchers may use slides or Petri dishes to perform imaging and microdissection experiments. Following a validated protocol from Arcturus® LCM Systems from Applied Biosystems—the leaders in tissue-based microdissection—the live cells may be isolated, remaining viable for subsequent re-culture or direct processing for use in downstream applications. Polyethylene naphthalate (PEN) Membrane Frame Slides for Live Cell Microdissection • Specially optimized for use with live cell expe...
Catalog: LCM0531
Size: 25 each
Size: 25 each
The lipophilic probe, FM™ 4-64FX exhibits low fluorescence in water but fluoresces intensely upon binding the outer leaf of the plasma membrane providing discrete plasma membrane staining. The binding is rapid and reversible and "FX" denotes that this analog is fixable with aldehyde-based fixatives. It is also an excellent reagent for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling.
Catalog: F34653
Size: 10 × 100 µg
Size: 10 × 100 µg
The Violet Ratiometric Membrane Asymmetry Probe⁄Dead Cell Apoptosis Kit provides a simple and fast method for the detection of apoptosis with dead cell discrimination by flow cytometry. The Violet Ratiometric Membrane Asymmetry Probe, 4'-N,N-diethylamino-6-(N,N,N-dodecyl-methylamino-sulfopropyl)-methyl-3-hydroxyflavone (F2N12S), is a novel violet excitable dye for the detection of membrane asymmetry changes during apoptosis. The dye exhibits an excited-state intramolecular proton transfer (ESIPT) reaction resulting in a dual fluorescence with two emission bands corresponding to 530 nm and 585 ...
Catalog: A-35137
Size: 1 kit
Size: 1 kit
InVision™ His-tag In-gel Stain is a ready-to-use, fast, sensitive and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis. Using InVision™ His-tag In-gel Stain you'll: Stain proteins fused to an oligohistidine sequence Eliminate western blotting-detect His-tagged proteins directly in the gel Detect nanogram levels of protein bands with a UV transilluminator, equipped with a standard camera, or a laser-based scanner Visualize results in under 3 hours Convenienty provided as one ready-to-use solution, InVision™ His-tag ...
Catalog: LC6030
Size: 500 ml
Size: 500 ml
NuPAGE® Novex® 3–8% Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex® Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Formulation NuPAGE® Novex® Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended buffers Run NuPAGE® Novex® Tris-...
Catalog: EA03785BOX
Size: 1 box
Size: 1 box
The PichiaPink™ Secretion Signal Set is a set of 8 DNA fragments encoding secretion signal sequences. The PichiaPink™ Secretion Signal Set is designed to be easily ligated in front of your gene. The PichiaPink™ Secretion Signal Set is designed for use with the pPINK-HC and pPINK-LC vectors. Optimize Secretion of Your Expressed Protein By adding one of the 8 secretion signal sequences to your gene, you can secrete your protein into the media to make it easier to harvest and purify. Pichia pastoris secretes few native proteins, so the bulk of the protein in the media will be yours. Each DNA sequ...
Catalog: A11155
Size: 8 each
Size: 8 each
NuPAGE® Novex® 3–8% Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex® Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Formulation NuPAGE® Novex® Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended buffers Run NuPAGE® Novex® Tris-...
Catalog: EA0376BOX
Size: 1 box
Size: 1 box
The Pippin Prep™ Kit 2010 contains 10 disposable pre-cast 3% agarose gel cassettes and a reagent pack for use isolating DNA fragments in the 50 - 300 bp range. The gel cassettes have 5 lanes, allowing 4 samples to be run along with an external size standard. The reagent pack includes that external standard as well as loading solution and electrophoresis buffer. Pippen Prep™ Kits can be purchased separately and are available in three different formats. Streamlined Pippin Prep™ System DNA Size Selection Process The workflow for Pippin Prep™ DNA size selection consists of just a few simple steps:...
Catalog: 4472172
Size: 10 reactions
Size: 10 reactions
NuPAGE® Novex® 7% Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex® Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Formulation NuPAGE® Novex® Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended buffers Run NuPAGE® Novex® Tris-Ac...
Catalog: EA03552BOX
Size: 1 box
Size: 1 box
Pro-Q Diamond phosphoprotein gel stain provides a convenient method for selectively staining phosphoproteins in acrylamide gels, without the need for blotting or phosphoprotein specific antibodies and Western blot analysis. Preparation of the Pro-Q Diamond phosphoprotein gel destaining solution is described in the gel staining protocol, but we also provide the detaining solution premixed for your convenience.
Catalog: P-33310
Size: 1 L
Size: 1 L
Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 7.5 cm x 8.4 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to m...
Catalog: LC2010
Size: 50 sheets
Size: 50 sheets
The PichiaPink™ Secreted Vector Kit contains the pPINKα-HC vector for use with the PichiaPink™ Yeast Expression System. The pPINKα-HC vector is built around the ADE2 gene for complementing the complementing the ade2 deletion in the PichiaPink™ strains. The pPINKα-HC vector has the α-mating factor secretion signal sequence so that all proteins are secreted. You can order the PichiaPink™ Secreted Vector Kit to refill your PichiaPink™ Yeast Expression System Kit. The PichiaPink™ Secreted Vector Kit comes with: • pPINKα-HC vector (high-copy number) (20 µg) • 5’α-factor sequencing primer (20 µg) • ...
Catalog: A11153
Size: 1 kit
Size: 1 kit
NuPAGE® Novex Bis-Tris Gels provide the best separation and resolution of small- to medium-sized proteins by utilizing a neutral pH environment which minimizes protein modifications. These high-performance gels have a one-year shelf life when stored at +4°C to 25°C, eliminating the waste of throwing out expired gels. Use NuPAGE® Novex Bis-Tris Gels for protein sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. The NuPAGE® System also provides the most efficient means for transferring proteins to membranes for subsequent analysis. Formulation: The NuPAGE...
Catalog: NP0330BOX
Size: 1 box
Size: 1 box
Voltage Sensor Probes (VSPs) are a Fluorescence Resonance Energy Transfer (FRET)-based voltage-sensing assay technology used to measure changes in cellular membrane electrical potential. VSPs are ideal for screening compounds that modulate ion channels because they provide: High-throughput capabilities-screen 32,000 samples in one day Highly sensitive detection-accurately and precisely report a 1% change in the Emission Ratio per mV Rapid detection response-sub-second time response Ratiometric readout (the Emission Ratio)-reduces experimental errors arising from well-to-well variations in cell...
Catalog: K1022
Size: 5 mg
Size: 5 mg
Yeast and Cellmask and Plasma Membrane
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