The Vybrant Apoptosis Assay Kit #4 detects apoptosis on the basis of changes that occur in the permeability of cell membranes. This kit contains ready-to-use solutions of both YO-PRO-1 and propidium iodide nucleic acid stains. YO-PRO-1 stain selectively passes through the plasma membranes of apoptotic cells and labels them with moderate green fluorescence. Necrotic cells are stained red-fluorescent with propidium iodide.
Size: 1 kit
Mouse anti-Galectin-1; Monoclonal antibody specific to Human, mouse, swine, rat, ovine, hamster, bovine Galectin-1.This antibody is validated for use in Western Blot, Immunocytochemistry/ Immunofluorescence.
Size: 100 µg
This purified mouse anti-NY-ESO-1 monoclonal antibody recognizes human NY-ESO-1. NY-ESO-1 is a member of the cancer/testis (CT) family of antigens, which are expressed in a range of malignant neoplasmas such as melanoma and carcinomas of the breast, ovary and lung, but not in normal tissue except in testis. NY-ESO-1 is a classic CT antigen discovered during a SEREX analysis of esophageal cancer. It is a 22–23 kDa hydrophobic protein. Validated applications for anti-NY-ESO-1 are western blotting and immunohistochemistry (paraffin). Antibody Specifications: • Applications: Validated applications...
Size: 100 µg
The CD90/Thy-1 (clone G7) R-PE (phycoerythrin) conjugate is a monoclonal rat antibody that recognizes the Thy-1.1 and Thy-1.2 alloantigens (Thy-1 epitope region A). R-PE is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thereby affording exceptional quantum yields and molar extinction coefficients. The use of PE in antibody conjugates provides probes with greatly enhanced detectability. Antibody Specifications Description: CD90/Thy-1, Rat IgG 2c monoclonal Label: R-PE (phycoer...
Size: 25 µg
This is a purified rabbit anti-human Lipocalin-1 oligoclonal antibody (oAb) that can be used in validated applications such as western blotting (WB), immunocytochemistry (ICC), and indirect ELISA. ABfinity™ antibody characteristics include: • APPLICATIONS: This Lipocalin-1 Oligoclonal Antibody, ABfinity™ Recombinant has been validated for use in western blotting, immunocytochemistry, and indirect ELISA. • HOST SPECIES AND ISOTYPE: This is a recombinant rabbit oligoclonal IgG against human Lipocalin-1. • CLONE ID OF OLIGOCLONAL (oAb): The oligoclonal antibody clone is 14HCLC. • REACTIVITY: Alon...
Size: 100 µg
Ambion® Molecular biology grade, 1M Tris, pH 7.0 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contamina...
Size: 500 ml
Gibco® Trypsin 1:250 powder is an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength it is widely used for cell dissociation, during routine cell culture passaging, and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing Quality Testing Gibco® Trypsin 1:250 is tested for endotoxin, mycoplasma, bacterial, fungal and viral contamination. Other quality tests include multiple activity assays, ash analysis and mois...
Size: 100 g
Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Size: 500 ml
The Ion Xpress™ Barcode Adapters 1-16 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Ba...
Size: 1 kit
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. Life Technologies offers a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This M199 is modified as follows: With Without • L-glutamine • Phenol Red • HEPES
Size: 500 ml
Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® re...
Size: 2000 reactions
The cell-impermeant viability indicator ethdium homodimer-1 (EthD-1) is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (excitation/emission maxima ~528/617).
Size: 1 mg
Ambion® Acrylamide/Bis 19:1 is a 40% (w/v) solution of acrylamide (38%) and bis-acrylamide (2%) ideal for use in ribonuclease protection assay, sequencing gels, and sizing DNA or RNA fragments. Supplied in one bottle containing 500 mL. The solution is provided in a ready-to-use form, reducing the dust, inhalation, and contact hazard associated with weighing and preparing acrylamide and bis-acrylamide powders and solutions. Concentrations are carefully controlled and all solutions are micro-filtered prior to packaging. All Ambion® acrylamides are rigorously tested for contaminating non-specific...
Size: 500 ml
UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl. When mixtures are extracted with Phenol:Chloroform:Isoamyl Alcohol, proteins are denatured and collected in the organic phase or at the interphase, while nucleic acids remain in the aqueous phase. UltraPure™ Phenol:Chloroform:lsoamyl Alcohol contains no preservatives. It is packaged under an inert gas in shatter-resistant, plastic-coated amber bottles. Performance and Quality Te...
Size: 400 ml
The TaqMan® GTXpress™ Master Mix delivers accurate genotyping results with robust performance in less than 50 min. Applied Biosystems® TaqMan® GTXpress™ Master Mix (2X) combines AmpliTaq® Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format. The 1 mL pack includes reagents sufficient to perform 400 PCR reactions (5 µL/reaction). • Compatible with both Fast and Standard mode thermal cycling conditions • Pre-mixed components stored at 2°–8°C significantly reduce assay setup time • Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays •...
Size: 400 reactions
These 2 buffers are to be used only as a complement to the SuperScript® III Platinum® CellsDirect Kits when large number of samples are processed. See manual of SuperScript® III Platinum® CellsDirect Kits for protocol recommendations.
Size: 1 kit