Enrich epithelial tumor cells directly from whole blood, bone marrow, or PBMC suspensions. Enriched tumor cells are lysed for mRNA isolation and RT-PCR amplification. The sensitivity of combined enrichment and RT-PCR detection can be as high as 1 tumor cell in 1 ml blood. Depletion of epithelial tumor cells from whole blood/bone marrow or PBMC. Positive isolation of epithelial tumor cells for downstream molecular analysis. No columns required Starting samples: Whole blood, bone marrow, PBMC, buffy coat. Number of cells processed: 200 ml whole blood or ~4 × 10 9 PBMC.
Size: 5 ml
N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein’s N-1 formulation. N-2 Supplement is intended for use with Neurobasal® media. N-2 Supplement is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). N-2 Supplement can be used as a substitute for Bottenstein’s N-1 formulation. N-2 Supplement is provided as a 100X concentrate. N-2 Supplement may be used with Neurobasal® media supplemented with growth factors such as bFGF and E...
Size: 5 ml