Topo Pcr Cloning Vectors

Zero Blunt® TOPO® PCR Cloning Kit (with pCR®-Blunt II-TOPO®) with One Shot® TOP10 Chemically Competent E. coli and PureLink™ Quick Plasmid Miniprep Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > PCR Cloning Vectors

Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR®-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase (1). The pCR®-Blunt II-TOPO® Vector also features: • ccdB gene for positive selection (2) • Eco R I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli • SP6 promoter/primer site for...
Catalog: K2800-02
Size: 20 preps

TOPO® TA Cloning® Kit for Sequencing, with One Shot® Mach1™ T1 Phage-Resistant Chemically Competent E. coli

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > PCR Cloning Vectors

The TOPO® TA Cloning® Kits for Sequencing provide a highly efficient, 5 minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO® TA vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction. These kits include everything necessary to clone and select recombinant vectors containing your PCR fragment of choice. Get More Sequence— Allows for more insert sequence and less vector seuquence when usin...
Catalog: K4530-20
Size: 20 reactions

PENTR/D-TOPO CLONING KIT

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > PCR Cloning Vectors

PENTR/D-TOPO CLONING KIT
Catalog: K240020SP
Size: 20 reactions

PCR8/GW/TOPO

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > PCR Cloning Vectors

PCR8/GW/TOPO
Catalog: K252020SP
Size: 20 reactions

pCR®8/GW/TOPO® TA Cloning Kit with One Shot® TOP10 E. coli

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Gateway® Entry Vectors - pENTR

The pCR®8/GW/TOPO® TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway® recombination cloning vector platform. Our pCR®8 TOPO® TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector. With our patented att sites, the pCR®8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway® recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time. The pCR®8/GW/TOPO® TA Cloning Kit offers: att sites for rapid recombination into a variety of Gateway...
Catalog: K250020
Size: 20 reactions