Topo DNA⁄Rna Polymerases
T7 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage T7 promoter sequences. The enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available. Application: Synthesis of labeled and unlabeled RNA transcripts (1). Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid. Performance and Quality Testing: 3´ and 5´ exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction. Unit De...
Catalog: 18033-100
Size: 2 × 2,500 units
Size: 2 × 2,500 units
RNAlater®-ICE is a novel reagent for transitioning frozen tissue to a state that is easily processed for extraction of high quality RNA. Frozen tissues are simply submerged in RNAlater®-ICE and allowed to thaw overnight at -20°C. Once thawed, the tissues can be processed like fresh tissues using standard RNA isolation procedures. No more laborious grinding of frozen tissue to safeguard the RNA. • Process previously frozen tissues like freshly harvested samples • Thawing tissues in RNAlater®-ICE protects RNA from degradation • No more tissue pulverizing with mortar and pestle and awkward transf...
Catalog: 4427575
Size: 10 bottles
Size: 10 bottles
E. coli DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of β-NAD between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate cohesive termini. Single-stranded nucleic acids are not substrates for this enzyme. Applications: Second-strand cDNA synthesis (1). T4 DNA Ligase alternative when blunt-end ligation is not required (2). Source: Purified from E. coli 594 (Su - ) bearing λ lysogen gt4lop-11 lig + S7 (3). Performance and Quality Testing: Endodeoxyribonuclease and 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested. Unit Definition: One unit is the...
Catalog: 18052-019
Size: 100 units
Size: 100 units
THE Ambion® RNA Storage Solution is a buffer that provides greater RNA stability than standard 0.1 mM EDTA or TE Buffer. This 1 mM sodium citrate, pH 6.4 +/- 0.2 buffer is provided in one bottle containing 50 mL. The last step in every RNA isolation protocol is to resuspend the purified RNA pellet. After painstakingly isolating the RNA, it is crucial that the pellet be suspended and stored in a safe, RNase-free solution. THE RNA Storage Solution has two features that minimize base hydrolysis of RNA: a low pH, and sodium citrate, which is an efficient chelating agent. THE RNA Storage Solution i...
Catalog: AM7001
Size: 50 ml
Size: 50 ml
Ambion® TURBO DNA- free ™ contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. The kit includes sufficient reagents for 50 rxns. Features of Ambion® TURBO DNA- free ™ include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can plague RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The Best Method for Genomic DNA Removal Prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNa...
Catalog: AM1907
Size: 50 reactions
Size: 50 reactions
DNA End Repair Mix offers an easy, one step process for creating blunt ends in sheared, sonicated, or restriction-cut DNA and PCR fragments for efficient ligation into blunt end cloning vectors. DNA End Repair Mix allows you to: Generate blunt ends in DNA and PCR fragments (A-tail) Repair DNA with 5′ or 3′ overhangs in 30 minutes at 37°C Heat inactivate DNA End Repair Mix in 10 minutes at 75°C A Simple Way to Make Blunt Ends With DNA End Repair Mix, DNA with 5′ or 3′ overhangs is converted to 5′ phosphorylated blunt end DNA in 30 minutes at 37°C. Once DNA has been repaired, the enzymes in DNA ...
Catalog: A14321
Size: 20 reactions
Size: 20 reactions
Ambion® TURBO DNA- free ™ contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. The kit includes sufficient reagents for 50 rxns. Features of Ambion® TURBO DNA- free ™ include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can plague RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The Best Method for Genomic DNA Removal Prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNa...
Catalog: AM1907M
Size: 50 rxns w⁄ Manual
Size: 50 rxns w⁄ Manual
Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR®-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase (1). The pCR®-Blunt II-TOPO® Vector also features: • ccdB gene for positive selection (2) • Eco R I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli • SP6 promoter/primer site for...
Catalog: K2800-02
Size: 20 preps
Size: 20 preps
The PureLink® Viral RNA/DNA Mini Kit is a nucleic acid purification system designed for fast and easy isolation of viral RNA or DNA from cell-free samples, such as serum, plasma, and cerebrospinal fluid. Starting sample volumes can be as high as 500 µl and elution volumes as low as 10 µl, allowing for a 50-fold concentration of nucleic acids. The higher target concentration obtained using the PureLink® Viral RNA/ DNA Mini Kit provides greater sensitivity and accuracy in downstream detection procedures such as real-time PCR or endpoint analysis. Specially formulated buffers enable the purificat...
Catalog: 12280-050
Size: 50 preps
Size: 50 preps
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Catalog: K6025-01
Size: 20 reactions
Size: 20 reactions
The pcDNA™3.1/V5-His TOPO® TA Expression Kit offers one-step cloning of Taq -amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1/V5-His-TOPO® vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants. In addition, the vector includes the following features: Strong CMV promoter for high-level, constitutive expression. C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody. C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating...
Catalog: K4800-40
Size: 40 reactions
Size: 40 reactions
The pcDNA™3.1 Directional TOPO® Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO® for five-minute directional cloning and subsequent high-level expression. Directional Cloning technology facilitates expression experiments because: A proofreading enzyme is used for fewer errors in cloned genes Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientation In addition, pcDNA3.1D/V5-His-TOPO® provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion t...
Catalog: K4900-40
Size: 40 reactions
Size: 40 reactions
The pLenti6⁄V5 Directional TOPO® Cloning Kit contains the TOPO®-adapted ViraPower™ lentiviral expression vector, pLenti6⁄V5-D-TOPO® for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving high-level, constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells. Advantages • High efficiency and rapid cloning • Constitutive gene expression in dividing and non-dividing mammalian cells in vitro or in vivo • Produces replication-incompet...
Catalog: K4955-10
Size: 20 reactions
Size: 20 reactions
Invitrogen's™ pLenti6.3⁄V5™-TOPO®Cloning Kit is part of our ViraPower™ HiPerform™ Lentiviral Expression System (catalog K5310-00). The pLenti6.3⁄V5™-TOPO® -TA Cloning Kit contains the TOPO®- adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5™-TOPO® for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5™-TOPO® vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequenc...
Catalog: K5315-20
Size: 1 kit
Size: 1 kit
The pENTR™/SD/D-TOPO® Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO® Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway® System or the MultiSite Gateway® System. Blunt-end PCR products clone directionally into the pENTR™/SD/D-TOPO® entry vector at greater than 90% efficiency, and include an upstream Shine-Dalgarno sequence for expression in prokaryotes. The kit comes with everything necessary to clone and select your PCR amplified gene of interest. Gateway® System Ready - Rapidly shuttle cloned genes between multiple ve...
Catalog: K2420-20
Size: 20 reactions
Size: 20 reactions
The TOPO® TA Cloning® Kits for Sequencing provide a highly efficient, 5 minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO® TA vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction. These kits include everything necessary to clone and select recombinant vectors containing your PCR fragment of choice. Get More Sequence— Allows for more insert sequence and less vector seuquence when usin...
Catalog: K4530-20
Size: 20 reactions
Size: 20 reactions
The ViraPower™ Lentiviral Directional TOPO® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. It combines Invitrogen’s ViraPower™ Lentiviral and Directional TOPO® technologies to facilitate quick PCR-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6⁄V5-D-TOPO® vector has the CMV promoter for driving constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells. Advantages • Lentivirus...
Catalog: K4950-00
Size: 1 kit
Size: 1 kit
PENTR/D-TOPO CLONING KIT
Catalog: K240020SP
Size: 20 reactions
Size: 20 reactions
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Catalog: K4202-01
Size: 20 reactions
Size: 20 reactions
The highest-quality RNA available from Ambion®, it is DNase-treated and subjected to unsurpassed quality control standards. One tube containing 100 µg is provided at a concentration of 1 mg/mL. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects used to judge the quality of FirstChoice® RNAs. The samples are processed using Ambion® RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment is performed to ensure that the RNA is ready for use in any ...
Catalog: AM7846
Size: 100 µg
Size: 100 µg
Topo DNA⁄Rna Polymerases
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