Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Other Reactive Probes
The red-fluorescent tetramethylrhodamine (TAMRA) alkyne can be reacted with azides via a copper-catalyzed click reaction. Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves a copper-catalyzed triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the ...
Catalog: T10183
Size: 0.5 mg
Category: Products & Services > Oligos, Primers, Probes & Nucleotides
TaqMan® MGB Probe
Catalog: 4316034
Size: 6,000 pmol
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Mouse ACTB (actin, beta) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. It can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: Actb Re...
Catalog: 4352341E
Size: 1000 reactions
Category: Products & Services > Oligos, Primers, Probes & Nucleotides
TaqMan® MGB Probe
Catalog: 4316033
Size: 20,000 pmol
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Rat ACTB (actin, beta) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: ACTB RefSeq: NM_031144.2 Probe Exon Location...
Catalog: 4352340E
Size: 1000 reactions
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Human ACTB (beta actin) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: ACTB RefSeq: NM_0...
Catalog: 4326315E
Size: 1000 reactions
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Human PGK1 (phosphoglycerate kinase 1) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: PG...
Catalog: 4326318E
Size: 1000 reactions
Category: Products & Services > Controls, Standards & Ladders > DNA Control Probes
Applications: ISH
Catalog: Q151P.0100
Size: 0.5 ml
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Rat GAPD (GAPDH) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. It can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: GAPDH RefSeq: NM_017008.3 Probe Exon Location: 3 Ampl...
Catalog: 4352338E
Size: 1000 reactions
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Eukaryotic 18S rRNA Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan Gene Expression Assays, Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: 18S RefSeq: X03205.1 Probe Exo...
Catalog: 4319413E
Size: 1000 reactions
Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > Fragment Analysis Size Standards
GeneScan™ 500 TAMRA™ Size Standard is an internal lane size standard developed for use with the Applied Biosystems fluorescence- based DNA electrophoresis systems. The use of an internal lane size standard during electrophoresis enables automated data analysis and precise DNA fragment size comparisons between electrophoresis runs. GeneScan™ 500 TAMRA™ Size Standard is designed for sizing DNA fragments in the 35-500 bp range and provides 16 single-stranded labeled fragments of 35, 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490, and 500 bases. Each of the DNA fragments is lab...
Catalog: 401733
Size: 800 reactions
Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > Real-Time PCR Instrument Calibration Kits & Reagents
The 384-Well Dye Calibration Plate (TAMRA™) is used to maintain your real-time PCR instrument with 384-well block. Dye calibration should be run after lamp change. Please see your instrument user guide for full instructions on use.
Catalog: 4443619
Size: 1 plate
Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > Real-Time PCR Instrument Calibration Kits & Reagents
The 96-Well Dye Calibration Plate (TAMRA™) is used to maintain your real-time PCR instrument with standard 96-well block. Dye calibration should be run after lamp change. Please see your instrument user guide for full instructions on use.
Catalog: 4442918
Size: 1 plate
Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > Real-Time PCR Instrument Calibration Kits & Reagents
The Fast 96-Well Dye Calibration Plate (TAMRA™) is used to maintain your real-time PCR instrument with Fast 96-well block. Dye calibration should be run after lamp change. Please see your instrument user guide for full instructions on use.
Catalog: 4432632
Size: 1 plate
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Apoptosis Kits
The Violet Ratiometric Membrane Asymmetry Probe⁄Dead Cell Apoptosis Kit provides a simple and fast method for the detection of apoptosis with dead cell discrimination by flow cytometry. The Violet Ratiometric Membrane Asymmetry Probe, 4'-N,N-diethylamino-6-(N,N,N-dodecyl-methylamino-sulfopropyl)-methyl-3-hydroxyflavone (F2N12S), is a novel violet excitable dye for the detection of membrane asymmetry changes during apoptosis. The dye exhibits an excited-state intramolecular proton transfer (ESIPT) reaction resulting in a dual fluorescence with two emission bands corresponding to 530 nm and 585 ...
Catalog: A-35137
Size: 1 kit
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® TaqMan® Rodent GAPDH Control Reagents provide the components for using rodent GAPDH as a normalization control in applications with rat, mouse, and Chinese hamster cells. • Pre-designed primers and TaqMan® probe eliminate assay design • Rapid assay development guidelines can minimize optimization time • Separate components provide flexibility in assay set-up Comprehensive Guidelines Included Applied Biosystems® has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems® Real-Time PCR reagents and instrumentation. These guidelines are...
Catalog: 4308313
Size: 1000 reactions
Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents
Alexa Fluor 488® phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. • Selectively stains F-actin • Outstanding fluorescence performance • Excitation/Emission: 495/518 nm • Superior to antibody staining • Optimal for fixed and permeabilized samples Get Superior Results in Your Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Flu...
Catalog: A12379
Size: 300 units
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Sequencing Primers
Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions. Invitrogen offers a wide selection of single-strand primers that may be used in either single- or double-stranded sequencing protocols. All sequencing primers are non-phosphorylated and are supplied lyophilized. All primers are: Desalted and purified by gel filtration Assayed for function in automated sequencing reactions Supplied in quantity of 2 µg
Catalog: N520-02
Size: 2 µg
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Sequencing Primers
It is a sequencing primer to sequence mammalian expression vectors having the BGH polyadenylation signal. Primer is supplied as 2 µg which equals 358 pMoles. The melting temperature is 54.8°C. The sequence of the primer is: 5'd[TAGAAGGCACAGTCGAGG]3'
Catalog: N575-02
Size: 2 µg
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
Human TaqMan® Copy Number Reference Assays are run with human TaqMan® Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome. Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT. TaqMan® Copy Number Reference Assays are designed to unique genomic sequences in the reference genome assembly (NCBI Build 37/hg19) and are required for relative quantitation of copy number targets. TaqMan® Copy Number Assays quantitate the gene of interest and normalize t...
Catalog: 4403326
Size: 750 reactions