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E. coli DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of β-NAD between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate cohesive termini. Single-stranded nucleic acids are not substrates for this enzyme. Applications: Second-strand cDNA synthesis (1). T4 DNA Ligase alternative when blunt-end ligation is not required (2). Source: Purified from E. coli 594 (Su - ) bearing λ lysogen gt4lop-11 lig + S7 (3). Performance and Quality Testing: Endodeoxyribonuclease and 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested. Unit Definition: One unit is the...
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
This purified mouse anti-human HACE1 monoclonal antibody (mAb) that can be used in the validated application of immunoprecipitation (IP). Antibody characteristics include: • APPLICATIONS: HACE1 Monoclonal Antibody, Recombinant Biotinylated Fab is validated for use in immunopreciptation. • HOST SPECIES AND ISOTYPE: This is a recombinant Mouse monoclonal biotinylated Fab against human HACE1. • PRODUCT SIZE: 100 µg Specific for HACE1 HACE1 (HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1) is involved in Golgi membrane fusion and regulation of small GTPases. It acts as a re...
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
This is a purified mouse anti-human HACE1 monoclonal antibody (mAb) that can be used in the validated application of immunoprecipitation (IP). Recombinant antibody characteristics include: • APPLICATIONS: This HACE1 recombinant mouse mAb has been validated for use in IP. • HOST SPECIES AND ISOTYPE: This is a recombinant mouse monoclonal IgG1 antibody against human HACE1. • PRODUCT SIZE: 100 µg Specific for HACE1 HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 (HACE1) is a ubiquitination moderator involved in Golgi membrane fusion and regulation of small GTPases. During ...