T Cell Media

Total Exosome Isolation (from cell culture media)

Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents

Total Exosome Isolation (from cell culture media) enables fast and efficient enrichment of intact exosomes from cell culture media samples. • Maximize recovery of intact exosomes for any type of downstream application • Easily isolate exosomes using our simple and reliable protocol • Avoid time-consuming ultra-centrifugation • Maximize flexibility—can be scaled up or down depending on the sample size Isolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation (from cell culture media) enables simple and reliable concentration of intact exosomes from cel...
Catalog: 4478359
Size: 50 ml

Exosome - Human CD63 Isolation/Detection (from cell culture media)

Category: Products & Services > Beads & Microspheres > Beads for Organelle Isolation

Exosome – Human CD63 Isolation/Detection enables the purification of CD63+ exosomes (also called extracellular vesicles and multi-vesicular bodies) from pre-enriched cell culture samples. These exosomes can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting. The exosomes must be pre-enriched before isolation. This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent. • Obtain highly pure CD63+ exosomes • "See" your sample during handling • Easily scalable protocol • Detec...
Catalog: 106-06D
Size: 3 ml

DMEM, Low Glucose, GlutaMAX™, Pyruvate

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • Low Glucose • HEPES • Sodium Pyruvate • GlutaMAX™ • Phenol Red
Catalog: 10567-014
Size: 500 ml

IMDM, Powder

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Iscove's (IMDM)

Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. Life Technologies offers a variety of Gibco® IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This IMDM is modified as follows: With Without • L-glutamine • α-thioglycerol • Phenol Red • 2-mercaptoethanol
Catalog: 12200-036
Size: 10 × 1 L

Ham's F-12K (Kaighn's)

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > F-12 Nutrient Mixture

Ham's F-12K (Kaighn's) medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) medium was developed for primary human hepatocytes, as well as some rat and chicken liver cells in a reduced serum environment. This F-12K (Kaighn's) medium is manufactured as follows: With Without • L-glutamine • HEPES • Phenol Red
Catalog: 21127-030
Size: 10 × 500 ml

CD CHO Medium (1X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > CHO & DG44 Media

CD CHO medium is a protein-free, serum-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. With a proven track record of quality for more than 10 years, CD CHO medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates. When to Use CD CHO Medium • Your GS CHO cells are recommended to be grown in CD CHO medium • Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a reg...
Catalog: 10743-029
Size: 1,000 ml

MEM, 2X Temin's, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Modified Eagle Medium (MEM)

Temin's Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • L-glutamine • HEPES • Earle's salts • Phenol Red • Sodium Bicarbonate
Catalog: 11935-046
Size: 500 ml

Ham's F-12K (Kaighn's)

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > F-12 Nutrient Mixture

Ham's F-12K (Kaighn's) medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) medium was developed for primary human hepatocytes, as well as some rat and chicken liver cells in a reduced serum environment. This F-12K (Kaighn's) medium is manufactured as follows: With Without • L-glutamine • HEPES • Phenol Red
Catalog: 21127-022
Size: 500 ml

William's E Medium, no Glutamine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Williams' Media E

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. This media can also be used for growing hepatocyte cells of different species, such as human HepaRG™ cells. This William's E Medium is modified as follows: With Without • Phenol Red • L-glutamine • HEPES
Catalog: 12551-032
Size: 500 ml

Advanced DMEM

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology. i Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero. This Advanced DMEM is manufactured as follows: With Without • High glucose • L-glutamine • Non Essential Amino Acids • Sodium pyruvate • Phenol Red
Catalog: 12491-015
Size: 500 ml

Macrophage-SFM, (1X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Other Serum-Free & Specialty Media

Macrophage-SFM is a serum-free medium designed for the culture of human peripheral blood monocytes and macrophages (1). Contains L-glutamine, but no antibiotics.
Catalog: 12065-074
Size: 500 ml

MCDB 131 Medium, no Glutamine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > MCDB 131

MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HUVEC). MCDB 131 Medium is also used with other cell types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells. This MCDB 131 Medium is manufactured as follows: With Without • Phenol Red • L-glutamine • Sodium pyruvate • HEPES
Catalog: 10372-019
Size: 500 ml

PFHM-II Protein-Free Hybridoma Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Hybridoma Media

Gibco® Protein Free Hybridoma Medium II (PFHM II) is a serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® PFHM II features: • Ability to support suspension and stationary hybridomas and myelomas • Complete, ready-to-use formulation • Serum-free, protein-free formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-depende...
Catalog: 23600042
Size: 1 x 10L

Opti-MEM® Reduced Serum Medium, GlutaMAX™

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Opti-MEM® I

Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 51985-034
Size: 500 ml

YPD Broth, Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Yeast Media

Gibco® YPD Broth is a ready-to-use liquid growth media used for yeast culture. Our filter-sterilized Gibco® YPD Broth can be used for nearly all research applications that require a liquid yeast culturing step such as protein expression, cloning, and expansion. • Gibco® microbiological media • Convenient ready-to-use liquid format • 1 L size available YPD Broth Recipe YPD Broth is a commonly used yeast growth media for maintenance and propagation of Pichia pastoris ( P. pastoris ) and Saccharomyces cerevisae ( S. cerevisae ) yeast strains. Formulation per One Liter: 10 g Yeast extract 20 g Bac...
Catalog: A13745-01
Size: 1 L

Synth-a-Freeze® - Defined, Cryopreservation Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Cryopreservation Media

Synth-a-Freeze® is a defined, liquid cryopreservation medium containing 10% dimethylsulfoxide (DMSO) without antibiotics, antimycotics, hormones, growthfactors, serum or protein and buffered with HEPES and sodium bicarbonate. Synth-a-Freeze is intended for freezing and storing a variety of mammalian cell types including human keratinocytes, embryonic stem cells, neural stem cells and mesenchymal stem cells. It should not be used for melanocytes.
Catalog: A12542-01
Size: 50 ml

Advanced DMEM/F-12

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM⁄F-12 Media

Advanced DMEM / F-12 (Dulbecco's Modified Eagle Medium / Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM / F-12, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology. i Cells successfully cultured in Advanced DMEM / F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat. This Advanced DMEM / F-12 is manufactured as follows: With Without • Glucose • L-glutamine • Non Essential Amino Acids • HEPES • Sodium pyruvate • Phenol Red
Catalog: 12634-010
Size: 500 ml

DMEM, High Glucose, no Glutamine, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES
Catalog: 31053-028
Size: 500 ml

Opti-MEM® Reduced Serum Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Opti-MEM® I

Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 31985-088
Size: 10 × 500 ml