Synthetic Rna Controls
Applied Biosystems® TaqMan® Ribosomal RNA Control Reagents are designed to detect the 18S ribosomal RNA (rRNA) gene. The TaqMan® probe and primer sequences were designed following Rapid Assay Development Guidelines. The TaqMan® probe and primer sequences are conserved among a diverse group of eukaryotes including human, rat, mouse, Xenopus, Saccharomyces, Giardia, and Arabidopsis. Ribosomal RNA levels provide an endogenous control for PCR quantitation studies. • Pre-designed primers and TaqMan® probe eliminate assay design • Rapid Assay Development Guidelines can minimize optimization time • S...
Catalog: 4308329
Size: 1000 reactions
Size: 1000 reactions
Ambion® In Vivo siRNA Controls • Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts • Positive Control siRNAs functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability • For use in human, mouse, and rat cells What are Ambion® In Vivo siRNAs? Ambion® In Vivo siRNAs are designed using the proven Silencer ® Select algorithm and incorpora...
Catalog: 4459407
Size: 50 nmol
Size: 50 nmol
Ambion® validated GAPDH Positive Control siRNA and non-targeting Negative Control siRNAs with the same chemical modifications for enhanced efficacy as in other Silencer ® Select siRNAs. • Validated siRNA controls for optimizing siRNA experiments • GAPDH Positive Control functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer ® Select modifications for enhanced specificity • For use in human, mouse, and rat cells What are Silencer ® Select siRNAs? Silencer ®Select siRNAs are the next ...
Catalog: 4390843
Size: 5 nmol
Size: 5 nmol
Silencer ® Select MALAT1 Positive Control siRNA is extensively validated and an ideal control for many aspects of an siRNA experiment. 5 nmol is provided. • Premium-quality siRNA, highly purified and ready to use • Functionally tested in several common cell lines • Include Silencer ® Select modifications for enhanced specificity • For use in human, mouse, and rat cells What are Silencer ® Select siRNAs Silencer ® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications, to yield siRNAs with unrivalled efficacy, poten...
Catalog: 4455877
Size: 5 nmol
Size: 5 nmol
Ambion® Silencer ® Firefly Luciferase (GL2 + GL3) Positive Control siRNA is ideal for developing and optimizing siRNA experiments. The control is validated for use in human cell lines and optimized for mouse and rat. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to...
Catalog: AM4629
Size: 5 nmol
Size: 5 nmol
Ambion® In Vivo siRNA Controls • Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts • Positive Control siRNAs functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability • For use in human, mouse, and rat cells What are Ambion® In Vivo siRNAs? Ambion® In Vivo siRNAs are designed using the proven Silencer ® Select algorithm and incorpora...
Catalog: 4457287
Size: 5 nmol
Size: 5 nmol
Ambion® Silencer ® KIF11 (Eg5) Positive Control siRNA (Human, Mouse, Rat) is ideal for developing and optimizing siRNA experiments. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs....
Catalog: AM4639
Size: 5 nmol
Size: 5 nmol
Ambion® Silencer ® GFP (eGFP) Positive Control siRNA is ideal for developing and optimizing siRNA experiments. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using oth...
Catalog: AM4626
Size: 5 nmol
Size: 5 nmol
Ambion® Silencer® Negative Control #2 siRNA has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proven to have no significant effect on cell proliferation, viability, or morphology. 40 nmol is provided. • Nontargeting with limited sequence similarity to known genes • Validated for use in human, mouse, and rat cells • Functionally proven to have minimal effects on cell proliferation and viability • HPLC purified, duplexed, and ready to use Negative control siRNAs are essential for determining transfection effi...
Catalog: AM4637
Size: 40 nmol
Size: 40 nmol
RNAlater®-ICE is a novel reagent for transitioning frozen tissue to a state that is easily processed for extraction of high quality RNA. Frozen tissues are simply submerged in RNAlater®-ICE and allowed to thaw overnight at -20°C. Once thawed, the tissues can be processed like fresh tissues using standard RNA isolation procedures. No more laborious grinding of frozen tissue to safeguard the RNA. • Process previously frozen tissues like freshly harvested samples • Thawing tissues in RNAlater®-ICE protects RNA from degradation • No more tissue pulverizing with mortar and pestle and awkward transf...
Catalog: 4427575
Size: 10 bottles
Size: 10 bottles
0.5-10 Kb RNA Ladder is suitable for sizing single-stranded RNA ranging from 0.5 kb to 10 kb in glyoxal or formaldehyde agarose gels. The ladder consists of nine bands in even increments (0.5, 1, 1.5, 2, 3, 4, 6, 8, and 10 kb) to simplify size estimation. The 1.5 kb band is more intense than the other bands, allowing easy orientation and identification of RNA bands on the gel. All RNA molecules in the ladder are polyA-tailed (40 nt) for convenient use as control templates for cDNA synthesis.
Catalog: 15623-200
Size: 75 µg
Size: 75 µg
THE Ambion® RNA Storage Solution is a buffer that provides greater RNA stability than standard 0.1 mM EDTA or TE Buffer. This 1 mM sodium citrate, pH 6.4 +/- 0.2 buffer is provided in one bottle containing 50 mL. The last step in every RNA isolation protocol is to resuspend the purified RNA pellet. After painstakingly isolating the RNA, it is crucial that the pellet be suspended and stored in a safe, RNase-free solution. THE RNA Storage Solution has two features that minimize base hydrolysis of RNA: a low pH, and sodium citrate, which is an efficient chelating agent. THE RNA Storage Solution i...
Catalog: AM7001
Size: 50 ml
Size: 50 ml
0.1-2 Kb RNA Ladder is suitable for sizing single-stranded RNA from 0.1 to 2 kb in glyoxal or formaldehyde agarose gels. The ladder consists of ten bands in even increments (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.8, 1.0, 1.5, and 2.0 kb) to simplify size estimation. The 0.3 kb band is more intense than the other bands, allowing easy orientation and identification of RNA bands on the gel. All RNA molecules in the ladder are polyA-tailed (40 nt) for convenient use as control templates for cDNA synthesis.
Catalog: 15623-100
Size: 75 µg
Size: 75 µg
Variation in RNA expression data can be attributed to a variety of factors including the quality of the starting material, the level of cellularity and RNA yield, the platform employed, and the person performing the experiment. To control for these sources of variability, a common set of external RNA controls has been developed by the External RNA Controls Consortium (ERCC), an ad-hoc group of academic, private, and public organizations hosted by the National Institute of Standards and Technology (NIST). The controls consist of a set of unlabeled, polyadenylated transcripts designed to be adde...
Catalog: 4456740
Size: 1 kit
Size: 1 kit
Ambion® RNA secure ™ (patents pending) is a unique non-enzymatic reagent that will irreversibly inactivate RNases in solution. 10 mL supplied. • No post-treatment autoclaving • Safe, easy-to-use reagent inactivates RNases in solutions • Inactivation process can be repeated to protect against newly introduced contaminants • Compatible with downstream procedures, such as RT-PCR and in vitro transcription • Inactivate RNases in Tris and other solutions that cannot be treated with DEPC RNase contamination in reagents used for RNA isolation and analysis can contribute to experimental inconsistency ...
Catalog: AM7006
Size: 10 ml
Size: 10 ml
Ambion® Silencer ® GAPDH Positive Control siRNA (human, mouse, rat) is ideal for developing and optimizing siRNA experiments. The control is validated for use in human, mouse, and rat cell lines. 40 nmol is provided. • Validated siRNA controls for optimizing siRNA experiments • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer ® Select...
Catalog: AM4631
Size: 40 nmol
Size: 40 nmol
Ambion® Silencer ® SelectGAPDH Positive Control siRNA, In Vivo Ready is extensively validated and an ideal control for many aspects of an siRNA experiment. The control is highly purified as required for animal use. 250 nmol is provided. • Premium-quality siRNA, highly purified and ready to use • Functionally tested in several common cell lines • Include Silencer ® Select modifications for enhanced specificity • For use in human, mouse, and rat cells What are Silencer ® Select siRNAs? Silencer ® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algo...
Catalog: 4404024
Size: 250 nmol
Size: 250 nmol
Synthetic Rna Controls
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