sybr DNA primers & oligos

Oligo (dT) Primer (50 µM)

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Oligo(dT) Primers

These are the same Ambion® primers that are currently included in the RETROscript® Kit (SKU# AM1710). They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript® Kit.
Catalog: AM5730G
Size: 80 µl

BGH Reverse Primer

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Sequencing Primers

It is a sequencing primer to sequence mammalian expression vectors having the BGH polyadenylation signal. Primer is supplied as 2 µg which equals 358 pMoles. The melting temperature is 54.8°C. The sequence of the primer is: 5'd[TAGAAGGCACAGTCGAGG]3'
Catalog: N575-02
Size: 2 µg

Oligo d(T) 16 (50 µM)

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Oligo(dT) Primers

Applied Biosystems® Oligo (dT) 16 is a homo-oligomeric deoxyribonucleotide (poly dT) used for priming and reverse transcription of polyadenylated (poly A+) mRNA. Note : See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostic procedures.
Catalog: N8080128
Size: 1 kit

Random Primers

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Random Primers

Random Primers are oligodeoxyribonucleotides (mostly hexamers) used to prepare labeled DNA probes from templates for filter hybridization or in situ hybridization and to prime mRNAs with or without poly(A) for cDNA synthesis. These primers are truly random and are suitable for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates. To avoid storage buffer interference, Random Primers are supplied in a low-salt-concentration buffer. Performance and Quality Testing: Incorporation of a radioactively labeled nucleotide is verif...
Catalog: 48190-011
Size: 9 units

5500 W Barcode Conversion Primers 1-16 Kit

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Sequencing Primers

The 5500 W Barcode Conversion Primers 1-16 Kit contains barcoded primers for libraries constructed with truncated adaptors, such as Agilent Technologies' SureSelect™ libraries, for multiplex sequencing on 5500 W Series genetic analyzers. This kit does not contain 5500 W Conversion Enzyme and should be used in conjunction with the 5500 W Conversion Primers Kit . This kit contains primers for 16 barcodes.
Catalog: 4481067
Size: 48 reactions

5500 W REV1 (DNA) SP Kit

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Sequencing Primers

The 5500 W REV1 (DNA) SP Kit contains sequencing primers and probes for DNA fragment libraries used in paired-end sequencing runs. The kit is used to prime the F5 tag for ligation sequencing on the 5500 W FlowChip V2 for DNA libraries. Each kit includes one strip tube containing sufficient specific sequencing primers and bridge probes to run 6 lanes.
Catalog: 4475684
Size: 1 kit

M13 Forward (-20)

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Sequencing Primers

Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions. Invitrogen offers a wide selection of single-strand primers that may be used in either single- or double-stranded sequencing protocols. All sequencing primers are non-phosphorylated and are supplied lyophilized. All primers are: Desalted and purified by gel filtration Assayed for function in automated sequencing reactions Supplied in quantity of 2 µg
Catalog: N520-02
Size: 2 µg

Random Decamers (50 µM)

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Random Primers

These are the same primers that are currently included in the RETROscript® Kit (SKU# AM1710). They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript® Kit.
Catalog: AM5722G
Size: 80 µl

EcoRI⁄MseI AFLP® Adaptors

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > AFLP Primer Sets

These individual EcoRI & MseI Adaptors can be used in the Restriction Ligation reaction. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 403077
Size: 100 reactions

Random Hexamers (50 µM)

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Random Primers

Applied Biosystems® Random Hexamers are short oligodeoxyribonucleotides of random sequence [d(N) 6 ] that anneal to random complementary sites on a target DNA or RNA, to serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase. With radiolabeled nucleotides present, the DNA is labeled quickly and reliably to high specific activity. Note : See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostics procedures.
Catalog: N8080127
Size: 1 kit

5500 W REV1 (RNA) SP Kit

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Sequencing Primers

The 5500 W REV1 (RNA) SP Kit contains sequencing primers and probes for whole transcriptome libraries in paired-end sequencing runs. This kit is used to prime the F5 tag for ligation sequencing on the 5500 W FlowChip V2 for RNA libraries. Each kit includes one strip tube containing sufficient specific sequencing primers and bridge probes to run 6 lanes.
Catalog: 4475683
Size: 1 kit

5’-Fluorescent labeled oligo

Category: Products & Services > Oligos, Primers, Probes & Nucleotides

5’-Fluorescent labeled oligo: 10,000 picomoles
Catalog: 450007
Size: 10,000 pmol

5’ Phosphate labeled oligo

Category: Products & Services > Oligos, Primers, Probes & Nucleotides

5’ Phosphate labeled oligo
Catalog: 4326342
Size: 80,000 pmol

Primer 5'FLO 50 nmol

Category: Products & Services > Oligos, Primers, Probes & Nucleotides

FLO (50 NMOL)
Catalog: 10918027


E. coli DNA Ligase

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > DNA⁄RNA Modifying Enzymes

E. coli DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of β-NAD between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate cohesive termini. Single-stranded nucleic acids are not substrates for this enzyme. Applications: Second-strand cDNA synthesis (1). T4 DNA Ligase alternative when blunt-end ligation is not required (2). Source: Purified from E. coli 594 (Su - ) bearing λ lysogen gt4lop-11 lig + S7 (3). Performance and Quality Testing: Endodeoxyribonuclease and 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested. Unit Definition: One unit is the...
Catalog: 18052-019
Size: 100 units

TURBO DNA- free ™

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > DNA⁄RNA Modifying Enzymes

Ambion® TURBO DNA- free ™ contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. The kit includes sufficient reagents for 50 rxns. Features of Ambion® TURBO DNA- free ™ include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can plague RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The Best Method for Genomic DNA Removal Prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNa...
Catalog: AM1907
Size: 50 reactions

TrackIt™ 25 bp DNA Ladder

Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > DNA Ladders

The TrackIt™ format combines the most popular DNA Ladders with loading buffer in a single tube. This format minimizes time spent thawing, diluting, and adding sample buffer to your DNA ladders. Simply transfer your TrackIt™ DNA Ladder from the vial to the gel. These ladders are stable at room temperature and can be stored on your bench top. TrackIt™ DNA Ladders offer two tracking dyes, one that runs behind the sample and one that runs ahead of the sample. The tracking dyes serve as visual markers for following migration during electrophoresis and indicate when maximum resolution of the DNA fra...
Catalog: 10488-022
Size: 20 applications

Mouse ACTB (Actin, Beta) Endogenous Control (VIC® / MGB Probe, Primer Limited)

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The Applied Biosystems® Mouse ACTB (actin, beta) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. It can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: Actb Re...
Catalog: 4352341E
Size: 1000 reactions

Rat ACTB (actin, beta) Endogenous Control (VIC®/MGB Probe, Primer Limited)

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The Applied Biosystems® Rat ACTB (actin, beta) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: ACTB RefSeq: NM_031144.2 Probe Exon Location...
Catalog: 4352340E
Size: 1000 reactions