Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > Real-Time PCR Instrument Calibration Kits & Reagents
This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR® Green I, VIC®/JOE™, and ROX™ dyes on the StepOne™ Real-Time PCR System. Features of the StepOne™ Real-Time PCR System Spectral Calibration Kit include: • 48-well optical reaction plates preloaded with dyes (FAM™/SYBR® Green I, VIC®/JOE™, and ROX™) are provided for quick and easy calibration of the StepOne™ Real-Time PCR System • Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture • Includes the Passive Reference I dye standard for sig...
Catalog: 4371433
Size: 1 kit
Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > DNA Sequencing & Matrix Standards
The BigDye® Terminator v3.1 sequencing standard kit contains four tubes, each of which contains enough DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v3.1 to perform one spectral calibration⁄performance check and⁄or one control sequencing run on the Applied Biosystems® 3500 Series Genetic Analyzer. The DNA contained in the preparation has been lyophilized to maximize stability. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Catalog: 4404312
Size: 4 tubes
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® TaqMan® Rodent GAPDH Control Reagents provide the components for using rodent GAPDH as a normalization control in applications with rat, mouse, and Chinese hamster cells. • Pre-designed primers and TaqMan® probe eliminate assay design • Rapid assay development guidelines can minimize optimization time • Separate components provide flexibility in assay set-up Comprehensive Guidelines Included Applied Biosystems® has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems® Real-Time PCR reagents and instrumentation. These guidelines are...
Catalog: 4308313
Size: 1000 reactions
Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Positive Controls
Ambion® Silencer ® Firefly Luciferase (GL2 + GL3) Positive Control siRNA is ideal for developing and optimizing siRNA experiments. The control is validated for use in human cell lines and optimized for mouse and rat. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to...
Catalog: AM4629
Size: 5 nmol
Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > DNA Ladders
The TrackIt™ format combines the most popular DNA Ladders with loading buffer in a single tube. This format minimizes time spent thawing, diluting, and adding sample buffer to your DNA ladders. Simply transfer your TrackIt™ DNA Ladder from the vial to the gel. These ladders are stable at room temperature and can be stored on your bench top. TrackIt™ DNA Ladders offer two tracking dyes, one that runs behind the sample and one that runs ahead of the sample. The tracking dyes serve as visual markers for following migration during electrophoresis and indicate when maximum resolution of the DNA fra...
Catalog: 10488-022
Size: 20 applications
Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Negative Controls
Ambion® In Vivo siRNA Controls • Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts • Positive Control siRNAs functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability • For use in human, mouse, and rat cells What are Ambion® In Vivo siRNAs? Ambion® In Vivo siRNAs are designed using the proven Silencer ® Select algorithm and incorpora...
Catalog: 4457287
Size: 5 nmol
Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > DNA Sequencing & Matrix Standards
The DS-30 Matrix Standard kit contains 4 oligonucleotides respectively labeled with 6-FAM™, HEX™, NED™, and ROX™, pre-pooled in a single tube. This Matrix Standard Set is used to generate the "multicomponent matrix" required when analyzing fluorescent dye-labeled DNA fragments on the Applied Biosystems® 3100, 3130, and 3730 Series Systems. The Data Collection Software for these instruments uses the multicomponent matrix to automatically analyze the four different colored fluorescent dye-labeled samples in a single capillary. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4345827
Size: 8 runs
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Mouse ACTB (actin, beta) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. It can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: Actb Re...
Catalog: 4352341E
Size: 1000 reactions
Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Positive Controls
Ambion® In Vivo siRNA Controls • Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts • Positive Control siRNAs functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability • For use in human, mouse, and rat cells What are Ambion® In Vivo siRNAs? Ambion® In Vivo siRNAs are designed using the proven Silencer ® Select algorithm and incorpora...
Catalog: 4459407
Size: 50 nmol
Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > Sequencing Workflow Controls
The Ion Sphere™ Quality Control Kit furnishes a complete set of reagents for performing templated bead quality control using the Qubit® 2.0 Fluorometer. This comprehensive kit contains calibration controls, buffers, and a probe mix for quality assessment of pre-enriched and post-enriched template-positive Ion Spheres. The powerful combination of the Ion Sphere™ Quality Control Kit and the Qubit® 2.0 Fluorometer gives you greater experimental control and significant time and cost savings. By providing the confidence to generate high quality sequencing data with every run, this quality control p...
Catalog: 4468656
Size: 1 kit
Category: Products & Services > Controls, Standards & Ladders > Protein Standards & Ladders > Unstained Protein Standards
The HiMark™ Unstained High Molecular Weight (HMW) Protein Standard is designed for analysis of high molecular weight proteins on Novex® NuPAGE® Tris-Acetate Gels . • Comprehensive – Contains markers with a wide range of high molecular weights, providing 9 protein bands in the range of 30-460 kDa • Convenient – A ready-to-use formulation eliminates the need to heat, reduce, or add sample buffer prior to use • Compatible – Intended for use with NuPAGE® Novex 3-8% and 7% Tris-Acetate Gels under denaturing conditions* *Note: HiMark™ Unstained High Molecular Weight Protein Standard can also be used...
Catalog: LC5688
Size: 250 µl
Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Negative Controls
Ambion® validated GAPDH Positive Control siRNA and non-targeting Negative Control siRNAs with the same chemical modifications for enhanced efficacy as in other Silencer ® Select siRNAs. • Validated siRNA controls for optimizing siRNA experiments • GAPDH Positive Control functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer ® Select modifications for enhanced specificity • For use in human, mouse, and rat cells What are Silencer ® Select siRNAs? Silencer ®Select siRNAs are the next ...
Catalog: 4390843
Size: 5 nmol
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Endogenous Control Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. This collection of genes has been selected from literature searches and⁄or whole genome microarray tests carried ...
Catalog: 4426700
Size: 1 Fast plate
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Rat ACTB (actin, beta) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: ACTB RefSeq: NM_031144.2 Probe Exon Location...
Catalog: 4352340E
Size: 1000 reactions
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Human ACTB (beta actin) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: ACTB RefSeq: NM_0...
Catalog: 4326315E
Size: 1000 reactions
Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Positive Controls
Ambion® Silencer ® KIF11 (Eg5) Positive Control siRNA (Human, Mouse, Rat) is ideal for developing and optimizing siRNA experiments. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs....
Catalog: AM4639
Size: 5 nmol
Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > DNA Ladders
1 Kb Plus DNA ladder is a powerful tool for estimating the molecular weight of linear, double stranded DNA fragments. 1 Kb Plus DNA ladder is composed of 20 highly purified, double stranded DNA bands spanning 100 base pairs to 12,000 base pairs. 1 Kb Plus DNA ladder has 12 evenly spaced bands ranging from 1 Kb to 12 Kb, a quick orientation band at 1650 base pairs that forms a distinct doublet with the 2 Kb band, and seven bands of round sizes below 1 Kb. 1 Kb Plus DNA ladder is compatible with agarose gels you cast in your lab as well as E-Gel® pre-cast agarose gels. Round, easy to remember ba...
Catalog: 10787-026
Size: 1000 µg
Category: Products & Services > Controls, Standards & Ladders > Quality Controls for Clinical & Serology Testing Labs > Clinical Laboratory Controls & Panels
The AcroMetrix® KRAS FFPE Process Controls are intended for use in assessing the performance of the isolation, purification, and detection of nucleic acid associated with specific KRAS mutations. Routine use of the KRAS FFPE Process Controls enables laboratories to monitor day-to-day workflow precision and to identify possible variations such as lot-to-lot performance of assay reagents and operator variation. The AcroMetrix® KRAS FFPE Process Controls can also assist in identifying increases in random or systematic error in testing systems. The AcroMetrix® KRAS FFPE Process Controls have been ...
Catalog: 950450
Size: 8 vials
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Mouse Endogenous Control 96-well Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. The plate contains 16 commonly used housekeeping genes. The panel also contains 16 mouse orthologs of hum...
Catalog: 4426699
Size: 1 Fast plate
Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > Fragment Analysis Size Standards
GeneScan™ 1000 ROX™ Size Standard is an internal lane size standard developed for use with Applied Biosystems® fluorescence-based DNA electrophoresis systems. The use of an internal lane size standard during electrophoresis enables automated data analysis and precise DNA fragment size comparisons between electrophoresis runs. GeneScan™ 1000 ROX™ Size Standard is designed for sizing DNA fragments in the 47–946 bp range and provides labeled fragments of 47, 51, 55, 82, 85 93 99, 126, 136, 262, 293, 317, 439, 557, 692, 695 and 946 bases. Each of the DNA fragments is labeled with ROX™ fluorophore,...
Catalog: 401098
Size: 400 reactions