Sybr Agarose

UltraPure™ Agarose

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Agarose

UltraPure™ Agarose is a polysaccharide (see structure below) used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Agarose is ideal for resolving DNA and RNA fragments from 500 bp to > 20 kb. • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handling and less breakage • Can be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay) Improved Packaging The new environmentally ...
Catalog: 16500100
Size: 100 g

UltraPure™ Low Melting Point Agarose

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Agarose

UltraPure™ Low Melting Point Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Low Melting Point Agarose is ideal for resolving DNA and RNA fragments larger than 1,000 bp and for the recovery of nucleic acid fragments after electrophoresis, as it melts at 65.5°C (below the melting point of most nucleic acids). The solution remains fluid at 37°C and will set rapidly at temperatures below 25°C. • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handlin...
Catalog: 16520100
Size: 100 g

DNA Grade Agarose

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Agarose

DNA Grade Agarose has a high sieve capacity, providing optimal resolution of DNA during gel electrophoresis. The low sulfur content allows gels to be run faster without becoming brittle. Supplied as a powder that is reconstituted in 0.5X TBE for high-quality agarose gels.
Catalog: 75000-500
Size: 500 g

UltraPure™ Agarose

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Agarose

UltraPure™ Agarose is a polysaccharide (see structure below) used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Agarose is ideal for resolving DNA and RNA fragments from 500 bp to > 20 kb. • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handling and less breakage • Can be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay) Improved Packaging The new environmentally ...
Catalog: 16500500
Size: 500 g

4% Agarose

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Agarose

Gibco® 4% Agarose is an autoclaved, high purity, low melting point gel designed for plaque assays and the purification of baculovirus. The 4% Agarose Gel melts at 65 – 70°C and remains fluid at 37°C. When diluted with concentrated insect cell culture medium, Gibco® 4% Agarose produces a gel firm enough to immobilize local infections, yet ductile enough to allow for the efficient removal of plaques for clonal expansion. Product Use For Research Use Only. Not for use in diagnostic procedures.
Catalog: 18300-012
Size: 40 ml

Power SYBR® Green PCR Master Mix, 1-Pack (1 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. • Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR • The optimized formulation contains highly purified AmpliTaq Gold® D...
Catalog: 4367659
Size: 200 reactions

SYBR® Green PCR Master Mix, 1-Pack (1 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial. • Premixed components stored at 2–8°C significantly reduce assay setup time • SYBR® Green I dye detects double-stranded DNA, so specific probes are not required • AmpliTaq Gold® DNA polymerase minimizes nonspecific product formation to achieve superior performance • dUTP significant...
Catalog: 4309155
Size: 200 reactions

Goat Anti-Mouse IgG (H+L) agarose

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Affinity purified whole antibody goat anti-mouse IgG conjugated to 4% beaded, crosslinked agarose (Sepharose® CL-4B). To minimize cross-reactivity, this antibody has been absorbed against human IgG and human serum.
Catalog: A10516
Size: 2 ml

Power SYBR® Green RNA-to-C T ™ 1-Step Kit (Mini-Pack)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qRT-PCR Kits

Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR® Green RNA-to-C T ™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes: • AmpliTaq® Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA • An additive that red...
Catalog: 4391178
Size: 40 reactions

Glutathione Agarose, Linked Through Sulfur, sedimented bead suspension

Category: Products & Services > Columns, Resins & Plates > Chromatographic Columns & Media > Affinity Media & Standards

Glutathione agarose prepared by linking the sulfur atom of glutathione to crosslinked beaded agarose can be used for purification of GST fusion proteins. Each milliliter of gel can bind approximately 5-6 mg of boivine-liver GST. Adding excess free glutathione liberates the GST fragment from the matrix which can then be regenerated by washing with a high-salt buffer.
Catalog: G2879
Size: 10 ml

Pippin Prep™ Kit 3010

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Agarose Gels

The Pippin Prep™ Kit 2010 contains 10 disposable pre-cast 3% agarose gel cassettes and a reagent pack for use isolating DNA fragments in the 50 - 300 bp range. The gel cassettes have 5 lanes, allowing 4 samples to be run along with an external size standard. The reagent pack includes that external standard as well as loading solution and electrophoresis buffer. Pippen Prep™ Kits can be purchased separately and are available in three different formats. Streamlined Pippin Prep™ System DNA Size Selection Process The workflow for Pippin Prep™ DNA size selection consists of just a few simple steps:...
Catalog: 4472172
Size: 10 reactions

Pippin Prep™ 2010 CEF Kit

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Agarose Gels

The Pippin Prep™ Kit 2010 contains 10 disposable pre-cast, ethidium-free, 2% agarose gel cassettes and a reagent pack for use isolating DNA fragments in the 100 - 600 bp range. The gel cassettes have 5 lanes, allowing 4 samples to be run along with an external size standard. The reagent pack includes that external standard as well as loading solution and electrophoresis buffer. Pippen Prep™ Kits can be purchased separately and are available in three different formats. Streamlined Pippin Prep™ System DNA Size Selection Process The workflow for Pippin Prep™ DNA size selection consists of just a ...
Catalog: 4472171
Size: 10 reactions

StepOne™ Real-Time PCR System Spectral Calibration Kit

Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > Real-Time PCR Instrument Calibration Kits & Reagents

This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR® Green I, VIC®/JOE™, and ROX™ dyes on the StepOne™ Real-Time PCR System. Features of the StepOne™ Real-Time PCR System Spectral Calibration Kit include: • 48-well optical reaction plates preloaded with dyes (FAM™/SYBR® Green I, VIC®/JOE™, and ROX™) are provided for quick and easy calibration of the StepOne™ Real-Time PCR System • Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture • Includes the Passive Reference I dye standard for sig...
Catalog: 4371433
Size: 1 kit

E-Gel® Safe Imager™ Real-Time Transilluminator

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Gel Imagers & Digital Electrophoresis Systems

The E-Gel® Safe Imager™ Real-Time Transilluminator allows you to watch migration of DNA in real-time in E-Gels containing SYBR® Safe. It offers: • Real-Time monitoring of DNA migration • Improved safety for the user • Enhanced cloning efficiency of retrieved DNA Real-Time Transilluminator Designed for Safety and Cloning The E-Gel® Safe Imager™ Real-Time Transilluminator is designed to fit perfectly beneath the E-Gel® iBase™ Power System and is intended for use with E-Gels containing SYBR® Safe. Blue light emitted by the instrument is ideal for viewing DNA that is stained with SYBR® Safe. Unlik...
Catalog: G6500
Size: 1 each