Suspension Cells are Hard to Transfect

Lipofectamine® RNAiMAX Transfection Reagent

Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents

Lipofectamine® RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine® RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types. With Lipofectamine® RNAiMAX Transfection Reagent you will get: Superior transfection efficiency requiring lower RNAi concentrations leading to more effective gene knockdown with minimal non-specific effects Easy optimization due to minimal cytotoxicity across a 10-f...

Catalog: 13778-100
Size: 0.1 ml

Lipofectin® Transfection Reagent

Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents

Lipofectin® Transfection Reagent is the reagent of choice for transfectin of endothelial cells; some of our customer also use it for transfection of DNA, RNA or oligonucleotides into their cell line of choice. Lipofectin® has also been shown to work well in combination with PLUS® reagent for the transfection of HeLa cells (1).

Catalog: 18292-011
Size: 1 ml

Neon™ Transfection System 100µL Kit (50 Reactions)

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Electroporation Instrument Accessories

The Neon™ Transfection System 100 µl Kit is designed specifically for use with the Neon™ Transfection System device. Use this kit for transfection volumes of 100 µl, containing 5 × 10 5 – 2 × 10 6 adherent cells or 1 × 10 6 – 5 × 10 6 suspension cells. Cells that have been transfected using the 100 µl Neon™ Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol. This ready-to-use universal kit comes complete with enough buffer and tips to run up to 50 reactions (25 in duplicate) using optimized or user defined protocols. For Research...

Catalog: MPK10025
Size: 50 reactions

Neon™ Transfection System 10 µL Kit (192 Reactions)

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Electroporation Instrument Accessories

The Neon™ Transfection System 100 µl Kit is designed specifically for use with the Neon™ Transfection System device. Use this kit for transfection volumes of 10 µl, containing 5 × 10 4 – 2 × 10 5 adherent cells or 1 × 10 5 – 5 × 10 5 suspension cells. Cells that have been transfected using the 10 µl Neon™ Tips are then ready to be washed and plated into a multi-well culture dish. This ready-to-use universal kit comes complete with enough buffer and tips to run up to 192 reactions (96 in duplicate) using optimized or user defined protocols. For Research Use Only. Not for any animal or human the...

Catalog: MPK1096
Size: 192 reactions

PLUS™ Reagent

Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents

PLUS™ Reagent is used in conjunction with transfection reagents, such as Lipofectamine™, to enhance transfection in adherent cell lines. Some customers find when using Plus™ Reagent that less optimization of DNA concentration is needed to achieve high transfection performance. The addition of Plus™ Reagent may also reduce the quantities of DNA and lipid required by approximately one-half compared to lipid alone. PLUS™ Reagent was specifically designed to expand the array of transfectable cell types (1). PLUS™ Reagent used with Lipofectamine™ Reagent has been demonstrated to improve the transfe...

Catalog: 11514-015
Size: 0.85 ml

Lipofectamine® LTX & Plus Reagent

Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents

Lipofectamine® LTX Reagent offers a new, advanced solution for gene expression studies in hard-to-transfect cells. No other plasmid DNA-specific transfection reagent can match the efficiency, convenience, and gentleness of Lipofectamine® LTX Reagent for transfection of primary, difficult-to-transfect, and sensitive cell lines. Lipofectamine® LTX Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in experiments or cell lines. A certificate of origin may be obtained by contacting Technical Support. ...

Catalog: A12621
Size: 0.1 ml