superscript® vilo™ cdna synthesis kit

SuperScript® Double-Stranded cDNA Synthesis Kit

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

The SuperScript® Double-Stranded cDNA Synthesis Kit contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality double-stranded cDNA from total RNA or poly A + selected RNA (mRNA). The advanced capabilities of SuperScript® II Reverse Transcriptase, with its reduced RNase H activity, maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis. Features of the SuperScript® Double-Stranded cDNA Synthesis Kit include: • Reliability —Each buffer, reagent, and enzyme in the kit is of the same high quality you expect from Life Technologie...
Catalog: 11917-010
Size: 10 reactions

NCode™ VILO™ miRNA cDNA Synthesis Kit

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

The NCode™ VILO™ miRNA cDNA Synthesis Kit is part of a novel quantitative assay that provides flexibility, sensitivity, and specificity when quantitating miRNA expression. The system combines a carefully optimized polyadenylation reaction with the market-leading reverse transcriptase, SuperScript® III RT, in a universal first-strand cDNA synthesis reaction. Tried and tested SuperScript® III Reverse Transcriptase is formulated in an enhanced buffer system to provide you with the most reliable firststrand synthesis and higher cDNA yields, with sensitivity and accurate quantitation from 10pg to 1...
Catalog: A11193-050
Size: 50 reactions

SuperScript® First-Strand Synthesis System for RT-PCR

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

SuperScript® First-Strand Synthesis System for RT-PCR is optimized for sensitive RT-PCR from low-input RNA, Taq or Platinum® Taq DNA Polymerase for higher specificity PCR or with AccuPrime™ Pfx DNA Polymerase for high-fidelity cloning applications. The SuperScript® First-Strand Synthesis System for RT-PCR: Is optimized for RT-PCR Uses SuperScript® II RT for greater first-strand cDNA yields Detects as little as 50 to 100 molecules of RNA template from only 1 pg of total RNA (Figure 1) Includes oligo(dT) and random hexamers to prime cDNA synthesis for flexible use Amplifies first-strand cDNA pro...
Catalog: 11904-018
Size: 50 reactions

SuperScript® III First-Strand Synthesis System

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

The SuperScript® III First-Strand Synthesis System for RT-PCR provides all the necessary components for synthesis of high-quality first-strand cDNA from total or poly(A)+ RNA. Used with PCR, this functionally tested system enables you to detect rare messages and generate enough material for cloning. The SuperScript® III First-Strand Synthesis System: Uses SuperScript® III RT for higher cDNA yields (Figure 1) Includes all components needed for first-strand cDNA synthesis Consists of optimized reagents for more robust performance Contains a streamlined protocol Detects template molecules from as...
Catalog: 18080-051
Size: 50 reactions

SuperScript® III First-Strand Synthesis SuperMix

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

The SuperScript® III First-Strand Synthesis SuperMix provides high yields of first-strand cDNA in a convenient high-throughput supermix format. The simple, time-saving reaction set-up uses just two tubes-a 2X Reaction Mix and a SuperScript® III Enzyme Mix (Figure 1). An optional annealing buffer is provided for improved yields and sensitivity. The Enzyme Mix includes SuperScript® III Reverse Transcriptase to ensure higher cDNA yields, superior sensitivity-down to 0.1 pg (Figure 2)-and specificity, and compatibility with a wide variety of RT-PCR applications.
Catalog: 18080-400
Size: 50 reactions