streptavidin-coupled dynabeads®
Streptavidin-coupled Dynabeads® is the gold standard for isolation and handling of biotinylated nucleic acids, antibodies or other biotinylated ligands and targets. The very high binding affinity of the streptavidin-biotin interaction (Kd=10 -15 ) is utilized in a vast number of applications. These uniform and superparamagnetic beads are 1 µm in diameter, with a monolayer - not a multilayer - of recombinant streptavidin covalently coupled to the surface and further blocked with BSA. The monolayer of streptavidin leaves the vast majority of the biotin binding sites sterically available for bind...
Catalog: 65602
Size: 10 ml
Size: 10 ml
Dynabeads® Sheep-anti Rat IgG binds monoclonal rat IgGs of your choice for specific cell isolation or depletion. All cells from all species except from rat can be isolated with this product, depending only on the specific antibody of choice. Especially well suited for cell isolation from mouse samples. Depletion: cells can be depleted from a sample Positive isolation: cells can be positively isolated from a sample for molecular applications Negative isolation: cells can be negatively isolated from a sample (i.e. spleen or lymph node cells) by adding an antibody cocktail of rat IgGs towards the...
Catalog: 11035
Size: 5 ml
Size: 5 ml
Our streptavidin, Marina Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ~365/460 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.
Catalog: S-11221
Size: 1 mg
Size: 1 mg
An excellent tool for highly predictable and consistent extraction and isolation of nucleic acids from biological samples, following a simple magnetic separation protocol. Please note that this is a stand-alone product containing only Dynabeads® in a storage buffer. Our products Dynabeads® SILANE genomic DNA (Cat.no. 370-12D) or Dynabeads® SILANE viral NA (Cat.no. 370-11D) include the appropriate buffers and protocols you need to extract genomic DNA or viral nucleic acids respectively. Magnetic particles from alternative suppliers often have a random size range distribution, surface area and b...
Catalog: 37002D
Size: 5 ml
Size: 5 ml
Reactivity: FITC-SA will react with biotin.
Catalog: 43-4311
Size: 1 mg
Size: 1 mg
Dynabeads® CD4 are magnetic beads that provide a robust and versatile tool to isolate or deplete human CD4 + T-cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. • Rapid isolation of CD4 + T-cells from any sample – no columns required • Positive isolation for molecular downstream assays or efficient depletion of CD4 + T cells Excellent Yield, Purity, and Viability of Recovered Cells Dynabeads® CD4 are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of human CD4 + cells directly from any samp...
Catalog: 11145D
Size: 5 ml
Size: 5 ml
The pcDNA™6 BioEase™ Gateway® Biotinylation System provides an easy, efficient method for expressing, purifying, and detecting biotinylated recombinant proteins. The pcDNA™6/BioEase™-DEST vector (Figure 1) incorporates a 72 amino acid sequence from K. pneumoniae that directs in vivo biotinylation of a specific lysine residue. This K. pneumoniae tag is recognized and efficiently biotinylated in vivo by a native mammalian system similar to the enzyme Biotin Protein Ligase (BPL) in E. coli (1). Proteins produced from the pcDNA™6/BioEase™-DEST vector are expressed as fusion proteins to this sequen...
Catalog: SA100-02
Size: 50 µl
Size: 50 µl
Streptavidin-Horseradish Peroxidase (HRP) conjugate
Catalog: SA100-01
Size: 50 µl
Size: 50 µl
This enzyme conjugate solution can be used to stain biotinylated antibodies.
Catalog: 50-209Z
Size: 15 ml
Size: 15 ml
Reactivity: CY5-SA reacts with biotin.
Catalog: 43-4316
Size: 1 mg
Size: 1 mg
Enrich epithelial tumor cells directly from whole blood, bone marrow, or PBMC suspensions. Enriched tumor cells are lysed for mRNA isolation and RT-PCR amplification. The sensitivity of combined enrichment and RT-PCR detection can be as high as 1 tumor cell in 1 ml blood. Depletion of epithelial tumor cells from whole blood/bone marrow or PBMC. Positive isolation of epithelial tumor cells for downstream molecular analysis. No columns required Starting samples: Whole blood, bone marrow, PBMC, buffy coat. Number of cells processed: 200 ml whole blood or ~4 × 10 9 PBMC.
Catalog: 16102
Size: 5 ml
Size: 5 ml
Reactivity: CY3-SA reacts with biotin.
Catalog: 43-4315
Size: 1 ml
Size: 1 ml
Alexa Fluor® 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western ...
Catalog: S-21374
Size: 1 mg
Size: 1 mg
Alexa Fluor® 488 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western ...
Catalog: S-32354
Size: 0.5 ml
Size: 0.5 ml
Use this kit to isolate a high purity and yield of human CD34+ progenitor stem cells. Positively isolated cells are bead and antibody-free, phenotypically unaltered and suitable for any downstream applications including flow cytometry, functional studies, and cell culture to produce dendritic cells (DC's). The Dynal® CD34 Progenitor Cell Isolation System can also be used to isolate CD34+ cells from rhesus monkey (Macaca mulatta). Positive isolation of human CD34+ progenitor stem cells with bead release Stem cells can be isolated directly from whole blood, cord blood or bone marrow Isolated CD3...
Catalog: 11301D
Size: 5 ml
Size: 5 ml
Qdot® 625 Streptavidin Conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot® nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including west...
Catalog: Q22063
Size: 50 µl
Size: 50 µl
Our Streptavidin, Pacific Green™ conjugate, is a Molecular Probes® fluorescent probe that absorbs at at 411 nm and is optimally excited by the violet laser. Streptavidin is a non-glycosylated biotin-binding protein isolated from bacteria with a near-neutral isoelectric point. It is widely used to detect biotinylated probes because it reportedly exhibits less non-specific binding than the glycosylated, biotin-binding protein avidin.
Catalog: S11200
Size: 500 µl
Size: 500 µl
Our Magnetic Particle Concentrators (MPC®) enable optimal binding of Dynabeads® magnetic beads with their targets (e.g., cells, proteins, and nucleic acids) in a variety of starting samples. The MPC®-S was designed specifically for microbiology applications; it has been validated with select Dynabeads® for enrichment of E. coli O157:H7, Salmonella, non-O157 STEC, Cryptosporidium, and Giardia. The MPC®-S can be used with both food enrichment samples types as well as water samples. It holds up to six microcentrifuge tubes and has an optimal working volume range of 20 µl–2 ml. Note that this magn...
Catalog: A13346
Size: 1 unit
Size: 1 unit
Exosome - Streptavidin for Isolation/Detection, when combined with your choice of biotinylated primary antibody, enables the purification of exosomes (also called extracellular vesicles and multi-vesicular bodies) from pre-enriched samples. These exosomes can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting. The exosomes must be pre-enriched before isolation. This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent. • Maximum flexibility with your choice of biotinylate...
Catalog: 106-08D
Size: 3 ml
Size: 3 ml
The Ion AmpliSeq™ Dynabeads® Cleanup Module is a magnetic bead-based purification kit used in the construction of RNA libraries for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ sequencer. This module is included in the Ion AmpliSeq™ RNA Library Kit, but may be purchased separately if additional modules are needed. For RNA library construction using the Ion AmpliSeq™ RNA Library Kit, the module is used for cleanup after the cDNA synthesis and PCR amplification steps. The module employs Dynabeads® magnetic beads for DNA adsorption and separation, rather than traditional f...
Catalog: 4482437
Size: 96 reactions
Size: 96 reactions
streptavidin-coupled dynabeads®
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