Sox Chelation-Enhanced Fluorophore
This concentrated Nitro-Block-II™ Enhancer can be used with CSPD® or CDP-Star® chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II™ membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF membranes. • Chemically optimized formulations provide enhanced signal intensity in blotting applications with dioxetane-based substrate solutions (CSPD® or CDP-Star® substrates) on nitrocellulose or PVDF membranes. • Enhancer increases signal intensity, allowing decreased signal acquisition time. Increa...
Catalog: T2184
Size: 20 ml
Size: 20 ml
Image-iT® FX Signal Enhancer ReadyProbes™ Reagent dramatically reduces background fluorescence resulting from non-specific staining during secondary detection using conjugates of streptavidin or secondary antibodies. This reagent is applied directly to slides or coverslips containing fixed and permeabilized cell or tissue samples prior to staining with fluorescent probes. Features of Image-iT® FX Signal Enhancer ReadyProbes™ Reagent include: • Easy protocol—apply ready-made solution using a dropper bottle • Compatible with the other steps in your immunostaining protocol • Effective with most f...
Catalog: R37107
Size: 1 kit
Size: 1 kit
The thiol-reactive Alexa Fluor 546 C 5 maleimide and its conjugates are perfect for applications that require orange-fluorescent probes. Spectrally similar to the Cy3 dye, the Alexa Fluor 546 dye is readily excited by the 546 nm line of the mercury-arc lamp and the 543 nm line of the He-Ne laser.
Catalog: A-10258
Size: 1 mg
Size: 1 mg
The amine-reactive Lissamine™ rhodamine B sulfonyl chloride can be used to create red-fluorescent bioconjugates with excitation/emission maxima ~568/583 nm.
Catalog: L-20
Size: 1 g
Size: 1 g
Our 4-acetamido-4'-maleimidylstilbene-2,2'- disulfonic acid is a thiol-reactive reagent that is water soluble, with high polarity and membrane impermeability. This polysulfonated dye is useful for determining whether thiol-containing proteins and polypeptide chains are exposed at the extracellular or cytoplasmic membrane surface.
Catalog: A-485
Size: 25 mg
Size: 25 mg
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: F-150
Size: 25 mg
Size: 25 mg
DTNB or Elman's reagent can be used to quantitate thiols in proteins, cells and plasma by absorption measurements. It readily forms a mixed disulfide with thiols, liberating the chromophore 5-merapto-2-nitrobenzoic acid (absorption maximum 410 nm). Only protein thiols that are accessible to this water-soluble reagent are modified.
Catalog: D-8451
Size: 10 g
Size: 10 g
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: C-6164
Size: 10 mg
Size: 10 mg
Tetrafluorophenyl (TFP) esters are an improvement over the succinimidyl ester (SE or NHS-ester) chemistry typically used to attach fluorophores or haptens to the primary amines of biomolecules. Both reactive chemistries produce the same strong amide bond between the dye or hapten and the compound of interest, but TFP esters are less susceptible to spontaneous hydrolysis during conjugation reactions. Alexa Fluor TFP esters are stable for several hours at the basic pH typically used for reactions – far outlasting succinimidyl esters.
Catalog: A-30005
Size: 1 mg
Size: 1 mg
Conjugates prepared with the thiol-reactive Alexa Fluor 647® C 2 maleimide are spectrally similar to the Cy5 dye, resulting in an optimal match to optical filters for the Cy5 dye. Total fluorescence of secondary antibody conjugates of Alexa Fluor® 647 dye, however, is significantly higher than that of Cy5 conjugates. Unlike the Cy5 dye, Alexa Fluor® 647 dye has very little change in absorption of fluorescence spectra when conjugated to most porteins, oligonucleotides, or nucleic acids, yielding greater total fluorescence at the same degree of substitution.
Catalog: A-20347
Size: 1 mg
Size: 1 mg
Conjugates prepared with the thiol-reactive Alexa Fluor 488 C 5 maleimide are significantly brighter and more photostable than fluorescein. The bright, green fluorescence of the Alexa Fluor 488 dye, which is spectrally similar to that of fluorescein, is pH independent from pH 4 to 10.
Catalog: A-10254
Size: 1 mg
Size: 1 mg
The amine-reactive PyMPO succinimidyl ester can be used to create bioconjugates with this environment-sensitive fluorophore.
Catalog: S-6110
Size: 5 mg
Size: 5 mg
The amine-reactive Alexa Fluor 430 carboxylic acid, succinimidyl ester and its conjugates exhibit the rare combination of absorption between 400 nm and 450 nm and fluorescence emission beyond 500 nm (excitation/emission maxima ~434/539 nm).
Catalog: A-10169
Size: 5 mg
Size: 5 mg
This single 5-isomer preparation of our proprietary, amine-reactive dye, Oregon Green 488 carboxylic acid, succinimidyl ester and its conjugates have green fluorescence similar to that of fluorescein but are more photostable. Additionally, Oregon Green 488 dye has a lower pK a than fluorescein (pK a = 4.7 versus 6.4 for fluorescein), making this bright dye less pH sensitive in the physiological range.
Catalog: O-6147
Size: 5 mg
Size: 5 mg
Pacific Green™ Succinimidyl Ester is a violet-excitable (411 nm) dye with an emission maximum at ~500 nm, allowing it to be used with other violet-excitable fluorophores (such as Pacific Blue™ or Pacific Orange™ dye) for multiparameter analysis using a single excitation source. As a class of compounds, succinimidyl esters offer an efficient and easy-to-use means for selectively linking a dye to accessible primary amine groups on proteins, modified nucleic acids, or other biomolecules. The covalent bonds formed are as stable as the peptide bonds used to link the amino acids within proteins. Pac...
Catalog: P11202
Size: 250 µg
Size: 250 µg
This is a purified rabbit anti-human p50 oligoclonal antibody that can be use in the validated applications of indirect ELISA, Western blot, and immunocytochemistry. Antibody characteristics include: Applications: This p50 Oligoclonal Antibody, ABfinity™ Recombinant has been validated for use in indirect ELISA, Western blot and immunocytochemistry. Host species and isotype: This is a recombinant rabbit oligoclonal IgG against human p50. Clone ID of monoclonal antibody (mAb): The p50 oligoclonal antibody clone is 24HCLC. Reactivity: Along with human p50, this oligoclonal antibody is predicted t...
Catalog: 710450
Size: 100 µg
Size: 100 µg
This is a purified rabbit anti-human smooth muscle actin oligoclonal antibody that can be use in the validated applications of indirect ELISA and Western blot. Antibody characteristics include: Applications: This Smooth Muscle Actin Oligoclonal Antibody, ABfinity™ Recombinant has been validated for use in indirect ELISA and Western blot Host species and isotype: This is a recombinant rabbit oligoclonal IgG against human smooth muscle actin. Clone ID of oligoclonal antibody (mAb): The smooth muscle actin oligoclonal antibody clone is 17HCLC. Reactivity: Along with human smooth muscle actin , th...
Catalog: 710460
Size: 100 µg
Size: 100 µg
Sox Chelation-Enhanced Fluorophore
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