Sequencing Kits & Reagents
The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337458
Size: 25000 reactions
Size: 25000 reactions
The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337457
Size: 5000 reactions
Size: 5000 reactions
The Ion AmpliSeq™ Comprehensive Cancer Panel provides highly multiplexed target selection of genes implicated in cancer research. Encompassing over 50% of the Wellcome Trust Sanger Institute Cancer Gene Census, this is the most comprehensive cancer gene panel available. With all-exon coverage of 409 genes, the Ion AmpliSeq™ Comprehensive Cancer Panel delivers fast, FFPE-compatible, target selection for a broad survey of key genes for semiconductor sequencing. Download the Ion AmpliSeq™ Comprehensive Cancer Panel list of target genes (.csv) . The Ion AmpliSeq™ technology allows ultra-high multi...
Catalog: 4477685
Size: 8 reactions
Size: 8 reactions
The Ion AmpliSeq™ RNA Apoptosis Panel is a screening tool designed against 267 genes involved in the cellular apoptosis pathway. Included are genes associated with death receptor-mediated apoptosis, c-Myc, and p53-mediated apoptosis. • Measure gene expression fold changes between samples • Amplicons are designed to be most inclusive for transcripts of the target gene • Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA • Prepare amplicon libraries with the Ion AmpliSeq™ RNA Library Kit and sequence on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system This...
Catalog: 4482571
Size: 24 reactions
Size: 24 reactions
The Ion AmpliSeq™ RNA Cancer Panel is a screening tool designed against 50 oncogenes/tumor suppressor genes found in the COSMIC database. This panel has been developed as an RNA complement to the DNA-focused Ion AmpliSeq™ Cancer Hotspot Panel v2. The panel is a single pool of primers representing 50 oncogenes and tumor suppressor genes with coverage of KRAS, BRAF, and EGFR genes. • Measure gene expression fold changes between samples • Amplicons are designed to be most inclusive for transcripts of the target gene • Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA •...
Catalog: 4482572
Size: 24 reactions
Size: 24 reactions
The BigDye® Terminator v3.1 sequencing standard kit contains four tubes, each of which contains enough DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v3.1 to perform one spectral calibration⁄performance check and⁄or one control sequencing run on the Applied Biosystems® 3500 Series Genetic Analyzer. The DNA contained in the preparation has been lyophilized to maximize stability. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Catalog: 4404312
Size: 4 tubes
Size: 4 tubes
The DS-30 Matrix Standard kit contains 4 oligonucleotides respectively labeled with 6-FAM™, HEX™, NED™, and ROX™, pre-pooled in a single tube. This Matrix Standard Set is used to generate the "multicomponent matrix" required when analyzing fluorescent dye-labeled DNA fragments on the Applied Biosystems® 3100, 3130, and 3730 Series Systems. The Data Collection Software for these instruments uses the multicomponent matrix to automatically analyze the four different colored fluorescent dye-labeled samples in a single capillary. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4345827
Size: 8 runs
Size: 8 runs
The Ion Sphere™ Quality Control Kit furnishes a complete set of reagents for performing templated bead quality control using the Qubit® 2.0 Fluorometer. This comprehensive kit contains calibration controls, buffers, and a probe mix for quality assessment of pre-enriched and post-enriched template-positive Ion Spheres. The powerful combination of the Ion Sphere™ Quality Control Kit and the Qubit® 2.0 Fluorometer gives you greater experimental control and significant time and cost savings. By providing the confidence to generate high quality sequencing data with every run, this quality control p...
Catalog: 4468656
Size: 1 kit
Size: 1 kit
The Ion Library Quantitation Kit is an easy-to-use, flexible solution for the accurate quantitation of library input for the Ion Torrent semiconductor sequencing workflow. This comprehensive kit provides a TaqMan® qPCR mix and library standard to detect and quantify femtomolar amounts of Ion Fragment Libraries. With universal cycling conditions that allow both fast and standard cycling, the Ion Library Quantitation kit supports most real-time platforms. This universal kit provides greater accuracy and specificity compared to non-probe-based methods, delivering simplicity and speed to the PGM™ ...
Catalog: 4468802
Size: 250 reactions
Size: 250 reactions
The Matrix Standard Kit is used to generate the "multicomponent matrix" required when analyzing 6-FAM™, VIC®, NED™, PET®, and LIZ®- labeled DNA fragments on the Applied Biosystems® 31xx, 3500 and 3730 Series Systems. The Data Collection Software for these instruments uses the multicomponent matrix to automatically analyze the five different colored fluorescent dye-labeled samples in a single capillary. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4345833
Size: 8 runs
Size: 8 runs
This kit contains 4 oligonucleotides respectively labeled with 6-FAM™, VIC®, NED™, and ROX™, pre-pooled in a single tube. Suitable for Dye Set D spectral calibration, on ABI PRISM® Analyzers: 3100-Avant™, 3100, and 3730. Supports 4-Dye fragment analysis applications (Linkage Mapping Set v2.5, Mouse Mapping Primers). The DS-31 Matrix Standard Set is used to generate the "multicomponent matrix" required when analyzing labeled DNA fragments on the Applied Biosystems® 3130 and 3100 Series Systems, 3700 DNA Analyzer, and 3730 Series Systems. The Data Collection Software for these instruments uses t...
Catalog: 4345829
Size: 8 runs
Size: 8 runs
The Ion PGM™ Controls Kit v2 contains control genomic DNA and sequencing controls for 3 reactions and control libraries for 100, 200, or 400 bp run configurations using the Ion Personal Genome Machine® (PGM™) semiconductor-based sequencing platform. The kit provides for greater experimental control through the use of quality control reagents for each step of the semiconductor-based sequencing workflow. The kit's quality control reagents allow you to assess sample quality, save time and reagents, and maximize productivity. The Ion PGM™ Controls Kit v2 provides you with: • Quality control tools ...
Catalog: 4482010
Size: 1 kit
Size: 1 kit
This kit includes all of the consumables and accessories needed to perform an instrument qualification—install, operational, or/and performance. This kit is specific for the 3500 Dx CS2 instrument and comes with a 50-cm 8-capillary array, POP-6™ polymer, and Big Dye® Terminator v1.1 sequencing standard.
Catalog: 4462042
Size: 1 kit
Size: 1 kit
This BigDye® Terminator v1.1 sequencing standard consists of four tubes, each of which contains DNA of a known 1,200 base pair sequence prepared with BigDye® Terminator v1.1. Sufficient DNA is included to perform one spectral calibration⁄performance check and⁄or one control sequencing run on an Applied Biosystems® 3500 Dx Series genetic analyzer. The DNA preparation has been lyophilized to maximize stability.
Catalog: 4462113
Size: 4 tubes
Size: 4 tubes
This kit includes all of the consumables and accessories needed to perform an instrument qualification—install, operational, or/and performance. This kit is specific for the 3500xL Dx CS2 instrument and comes with a 50-cm 24-capillary array, POP-6™ polymer, and Big Dye® Terminator v1.1 sequencing standard.
Catalog: 4462043
Size: 1 kit
Size: 1 kit
The Ion AmpliSeq™ Dynabeads® Cleanup Module is a magnetic bead-based purification kit used in the construction of RNA libraries for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ sequencer. This module is included in the Ion AmpliSeq™ RNA Library Kit, but may be purchased separately if additional modules are needed. For RNA library construction using the Ion AmpliSeq™ RNA Library Kit, the module is used for cleanup after the cDNA synthesis and PCR amplification steps. The module employs Dynabeads® magnetic beads for DNA adsorption and separation, rather than traditional f...
Catalog: 4482437
Size: 96 reactions
Size: 96 reactions
The DS-33 GeneScan™ Installation Standard consists of pooled PCR products labeled with 6-FAM™, VIC®, NED™ and PET® dyes. To generate the pooled products, CEPH individual 1347-02 genomic DNA has been amplified with 8 fluorescent PCR primer pairs which amplify selected microsatellite loci (as listed in the package product insert). The kit also contains a tube of GeneScan™ 600 LIZ™ Size Standard 2.0. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Catalog: 4376911
Size: 1 kit
Size: 1 kit
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