Sequencing Buffers & Reagents Chemicals
This sequencing buffer is optimized for use with BigDye® Terminator v1.1 and v3.1 Cycle Sequencing Kits. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4336701
Size: 233 ml
Size: 233 ml
This Casein-based blocking reagent can be used with Western-Light™, Western-Light Plus™, Western-Star™, ELISA-Light™, Southern-Light™, and Southern-Star™ Detection Systems. For Research Use Only. Not for use in diagnostics procedures.
Catalog: T2015
Size: 30 g
Size: 30 g
NuPAGE® Novex 3-8% Tris-Acetate Midi-Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. NuPAGE® Novex Tris-Acetate Midi-Gels (8 x 13 cm) can run up to 26 samples. The NuPAGE Midi-Gels run in the XCell4 Surelock™ Midi-Cell and, with a Midi Gel Adapter, can run in a Bio-Rad Criterion Cell. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity,...
Catalog: LA0050
Size: 1 kit
Size: 1 kit
NuPAGE® Transfer Buffer (20X) (125 ml) is a buffer used to transfer proteins from NuPAGE® gels to membranes for western blotting. NuPAGE® Transfer Buffer can be used with the Novex® Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. NuPAGE® Antioxidant may be used with NuPAGE® Transfer Buffer to enhance transfer of reduced proteins to membranes. For Research Use Only. Not intended for animal or human therapeutic or diagnostic use. Related Link • Learn more about other Invitrogen products for your protein transfer needs .
Catalog: NP0006
Size: 125 ml
Size: 125 ml
The NativePAGE™ Running Buffer Kit contains the NativePAGE™ Running Buffer (20X), which is used to make the NativePAGE™ Cathode and Anode Running Buffers for use with an XCell SureLock® Mini Cell when running NativePAGE™ Gels. The kit also contains the NativePAGE™ Cathode Buffer Additive (20X) that is used with the NativePAGE™ Running Buffer to make the Cathode Running Buffer for the system.
Catalog: BN2007
Size: 1 kit
Size: 1 kit
NuPAGE® Transfer Buffer (20X) (1 L) is a buffer used to transfer proteins from NuPAGE® gels to membranes for western blotting. NuPAGE® Transfer Buffer can be used with the Novex® Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. NuPAGE® Antioxidant may be used with NuPAGE® Transfer Buffer to enhance transfer of reduced proteins to membranes. For Research Use Only. Not intended for animal or human therapeutic or diagnostic use. Related Link • Learn more about other Invitrogen products for your protein transfer needs .
Catalog: NP0006-1
Size: 1 L
Size: 1 L
Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recomme...
Catalog: LC2676
Size: 20 ml
Size: 20 ml
The NuPAGE® MOPS SDS Running Buffer (20X) is formulated for running proteins on NuPAGE® Novex® Bis-Tris gels. NuPAGE® MOPS SDS Running Buffer is recommended for separating medium- to large-sized proteins. Use the Right Buffer to Optimize Protein Separations NuPAGE® MES SDS Running Buffer and NuPAGE® MOPS SDS Running Buffer can both be used with the NuPAGE® Bis-Tris gels. Using MOPS buffer allows proteins to run slower vs. MES buffer. Get Consistent, Convenient High-Quality Results Pre-mixed NuPAGE® buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buf...
Catalog: NP0001-02
Size: 5 L
Size: 5 L
Novex® Tris-Glycine Transfer Buffer (25X) is specially optimized for western blot transfer applications using Tris-Glycine gels.
Catalog: LC3675
Size: 500 ml
Size: 500 ml
NuPAGE® MES SDS Running Buffer (20X) is formulated for running proteins on NuPAGE® Novex® Bis-Tris gels. NuPAGE® MES SDS Running Buffer is recommended for separating small- to medium-sized proteins. Use the Right Buffer to Optimize Protein Separations NuPAGE® MES SDS Running Buffer and NuPAGE® MOPS SDS Running Buffer can both be used with the NuPAGE® Bis-Tris gels. Using MES buffer allows proteins to run faster vs. MOPS buffer. Get Consistent, Convenient High-Quality Results Pre-mixed NuPAGE® buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buffers a...
Catalog: NP0002
Size: 500 ml
Size: 500 ml
NuPAGE® Novex Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended Buffers: Run NuPAGE® Novex Tris-Acetate gels...
Catalog: LA0041
Size: 500 ml
Size: 500 ml
Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recomme...
Catalog: LC2675-5
Size: 5 L
Size: 5 L
NuPAGE® LDS Sample Buffer (4X) (10 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent. For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X) . Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sample...
Catalog: NP0007
Size: 10 ml
Size: 10 ml
NuPAGE® LDS Sample Buffer (4X) (250 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent. For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X) . Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sampl...
Catalog: NP0008
Size: 250 ml
Size: 250 ml
Bolt® Transfer Buffer is optimized for the transfer of proteins from Bolt® Bis-Tris Plus gels to membranes for western blotting. When combined with 10% methanol, Bolt® Transfer Buffer can be used with the Bolt® Mini Blot Module for wet transfer. Bolt® Transfer Buffer can also be used with Bolt® Antioxidant to enhance transfer of reduced proteins to membranes. About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout....
Catalog: BT0006
Size: 125 ml
Size: 125 ml
Bolt® Transfer Buffer is optimized for the transfer of proteins from Bolt® Bis-Tris Plus gels to membranes for western blotting. When combined with 10% methanol, Bolt® Transfer Buffer can be used with the Bolt® Mini Blot Module for wet transfer. Bolt® Transfer Buffer can also be used with Bolt® Antioxidant to enhance transfer of reduced proteins to membranes. About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout....
Catalog: BT00061
Size: 1 L
Size: 1 L
A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue. Supplied in one 1.4 mL tube. All Ambion® Gel Loading Solutions are rigorously tested for non-specific endonuclease activity, exonuclease activity, RNase activity, and for functionality. These are the same solutions we use in-house and in our kits.
Catalog: AM8546G
Size: 1 ml
Size: 1 ml
These 2 buffers are to be used only as a complement to the SuperScript® III Platinum® CellsDirect Kits when large number of samples are processed. See manual of SuperScript® III Platinum® CellsDirect Kits for protocol recommendations.
Catalog: 11739-010
Size: 1 kit
Size: 1 kit
Denaturing polyacrylamide TBE-Urea Gels resolve single-stranded DNA oligos or RNA into sharp, distinct bands. Novex® TBE-Urea Gels are optimized for the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies, and Northern blot analysis. Novex® TBE-Urea Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE-Urea Gels are made with high-purity reagents, including tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEME...
Catalog: LC6876
Size: 10 ml
Size: 10 ml
UltraPure™ DNA Typing Grade® 50X TAE Buffer is a sterile-filtered solution of 2 M Tris-acetate and 50 mM EDTA. TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. To prepare a 1X solution, mix one volume of UltraPure™ 50X TAE Buffer with 49 volumes of distilled water. A 1X TAE Buffer solution contains 40 mM Tris-acetate and 1 mM EDTA with a pH of 8.3 at 23“C. Performance and Quality Testing: No DNase activity detected.
Catalog: 24710-030
Size: 1 L
Size: 1 L
Sequencing Buffers & Reagents Chemicals
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