Sequencing Buffers & Reagents

BigDye® Terminator v1.1 & v3.1 5X Sequencing Buffer

Category: Products & Services > Buffers & Chemicals > Sequencing Buffers & Reagents

This sequencing buffer is optimized for use with BigDye® Terminator v1.1 and v3.1 Cycle Sequencing Kits. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4336701
Size: 233 ml

BigDye® Terminator v3.1 Cycle Sequencing Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337458
Size: 25000 reactions

BigDye® Terminator v3.1 Cycle Sequencing Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337457
Size: 5000 reactions

BigDye® Terminator v3.1 Cycle Sequencing Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337456
Size: 1000 reactions

Ion PI™ Sequencing 200 Kit v2

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The Ion PI™ Sequencing 200 Kit v2 contains reagents and consumables for running the single-day sequencing workflow on the Ion Proton™ Sequencer. As an integral component of the Ion Proton™ sequencing platform, the Ion PI™ Sequencing 200 Kit v2 is designed to sequence templated Ion PI™ Ion Sphere™ particles deposited in Ion PI™ chips for fast human exome resequencing or transcriptional profiling. The Ion PI™ Sequencing 200 Kit v2 provides you with: • Support for PI™ chips, enabling up to 80 million reads per run • Sequencing throughputs of 10 Gb or greater with 200 base read lengths • Low cost ...
Catalog: 4485149
Size: 8 reactions

I-Block™ Protein-Based Blocking Reagent

Category: Products & Services > Buffers & Chemicals > Immunoassay Buffers & Reagents > ELISA Buffers & Reagents

This Casein-based blocking reagent can be used with Western-Light™, Western-Light Plus™, Western-Star™, ELISA-Light™, Southern-Light™, and Southern-Star™ Detection Systems. For Research Use Only. Not for use in diagnostics procedures.
Catalog: T2015
Size: 30 g

Ion PGM™ Sequencing 400 Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The new Ion PGM™ Sequencing 400 Kit is an integral component of the Personal Genome Machine™ sequencing platform and single-day sequencing workflow, designed to sequence templated Ion Sphere™ particles deposited in Ion 314™ Chip v2, Ion 316™ Chip v2, and Ion 318™ Chip v2. This sequencing kit should be used in conjunction with Ion OneTouch™ 2 template preparation. The Ion PGM™ Sequencing 400 Kit provides you with: • Simple and natural sequencing chemistry • Low-cost standard reagents for lower cost per sample • Sequencing read lengths of 400 base pairs with short run times (up to 8.5 hours) • S...
Catalog: 4482002
Size: 4 sequencing runs

Ion PI™ Sequencing 200 Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The new Ion PI™ Sequencing Kit is an integral component of the Ion Proton™ sequencing platform and single-day sequencing workflow, designed to sequence templated Ion PI™ Ion Sphere™ particles deposited in Ion PI™ chips for human exome resequencing or transcriptional profiling. The Ion PI™ Sequencing 200 Kit provides you with: • Simple and natural sequencing chemistry • Low-cost standard reagents for lower cost per sample • Support for Ion PI™ chips • Support for multiplexed samples • Lower cost per run for a variety of applications, including exome and whole transcriptome sequencing The Bencht...
Catalog: 4482283
Size: 8 reactions

5500 W Paired-End Reagents Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The 5500 W Paired-End Reagents Kit is used for paired-end sequencing runs for ligation sequencing of the F5 Tag. This kit contains sufficient enzyme, dNTPs, and buffers for 6 lanes. In addition to 5500 W Run Cycle Buffers and 5500 W Buffer D (Reset), this kit requires the use of 5500 W Buffer H.
Catalog: 4475670
Size: 1 kit

Ion PI™ Chip Preparation Kit

Category: Products & Services > Buffers & Chemicals > Sequencing Buffers & Reagents

Ion PI™ Chip Preparation Kit contains solutions and materials for preparing the Ion PI™ Chip prior to sequencing on the Ion Proton™ System. The Ion PI™ Chip Preparation Kit is designed to prime the Ion PI™ Chip surface for optimal loading and performance for human exome resequencing or transcriptional profiling. The Ion PI™ Chip Preparation Kit provides: • Support for all Ion PI™ chips • Reproducibility for high quality sequencing • Maximum throughput for a variety of applications, including human exome resequencing and transcriptome analysis The Ion PI™ Chip Preparation Kit contains solutions...
Catalog: 4485415
Size: 8 reactions

Ion AmpliSeq™ Comprehensive Cancer Panel

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion AmpliSeq™ Comprehensive Cancer Panel provides highly multiplexed target selection of genes implicated in cancer research. Encompassing over 50% of the Wellcome Trust Sanger Institute Cancer Gene Census, this is the most comprehensive cancer gene panel available. With all-exon coverage of 409 genes, the Ion AmpliSeq™ Comprehensive Cancer Panel delivers fast, FFPE-compatible, target selection for a broad survey of key genes for semiconductor sequencing. Download the Ion AmpliSeq™ Comprehensive Cancer Panel list of target genes (.csv) . The Ion AmpliSeq™ technology allows ultra-high multi...
Catalog: 4477685
Size: 8 reactions

Ion PGM™ Enrichment Beads

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Templated Bead Preparation Kits & Reagents

Ion PGM™ Enrichment Beads are for use with Ion PGM™ template preparation kits for enrichment of template-positive Ion Sphere™Particles. The Ion PGM™ Enrichment Beads provide you with: • Simple enrichment for template-positive Ion Sphere™ Particles • Dynabeads® technology • Support for Ion PGM™ System workflow • Support for Ion OneTouch™ 200 Template Kit v2, Ion OneTouch™ Template Kit, Ion PGM™ 200 Xpress™ Template Kit, and Ion Xpress™ Template Kit The Ion PGM™ Enrichment Beads are streptavidin-coupled Dynabeads®, the gold standard for isolation due to their high binding affinity and uniformity...
Catalog: 4478525
Size: 10 reactions

Ion AmpliSeq™ RNA Apoptosis Panel

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion AmpliSeq™ RNA Apoptosis Panel is a screening tool designed against 267 genes involved in the cellular apoptosis pathway. Included are genes associated with death receptor-mediated apoptosis, c-Myc, and p53-mediated apoptosis. • Measure gene expression fold changes between samples • Amplicons are designed to be most inclusive for transcripts of the target gene • Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA • Prepare amplicon libraries with the Ion AmpliSeq™ RNA Library Kit and sequence on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system This...
Catalog: 4482571
Size: 24 reactions

Ion AmpliSeq™ RNA Library Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482335
Size: 8 reactions

Ion AmpliSeq™ RNA Library Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482752
Size: 384 reactions

Ion Xpress™ Barcode Adapters 1-16 Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion Xpress™ Barcode Adapters 1-16 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Ba...
Catalog: 4471250
Size: 1 kit

Ion Xpress™ Plus Fragment Library Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion Xpress™ Plus Fragment Library Kit provides a rapid and flexible enzymatic-based library construction methodology for the Personal Genome Machine™ sequencing system workflow. Employing enhanced proprietary Ion Shear™ DNA fragmentation chemistry, this revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries, removing the need for a physical shearing device to save time and cost. With significantly higher yields and lower bias than other library construction techniques, superior coverage uniformity is attained with as little as 100...
Catalog: 4471269
Size: 10 reactions

Ion OneTouch™ 200 Template Kit v2

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Templated Bead Preparation Kits & Reagents

The Ion OneTouch™ 200 Template Kit v2 contains template preparation reagents for automated and reproducible template preparation for 200 base-read libraries using the Ion OneTouch™ System . As an integral component of the semiconductor sequencing workflow, the Ion OneTouch™ 200 Template Kit v2 is designed to make high-quality templated Ion Sphere™ particles using the Ion OneTouch™ System to streamline the overall Ion sequencing workflow, yield significant time savings, increase productivity, and accelerate scientific discovery. The Ion OneTouch™ 200 Template Kit v2 provides you with: • Simple ...
Catalog: 4478316
Size: 10 reactions

Ion AmpliSeq™ RNA Cancer Panel

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion AmpliSeq™ RNA Cancer Panel is a screening tool designed against 50 oncogenes/tumor suppressor genes found in the COSMIC database. This panel has been developed as an RNA complement to the DNA-focused Ion AmpliSeq™ Cancer Hotspot Panel v2. The panel is a single pool of primers representing 50 oncogenes and tumor suppressor genes with coverage of KRAS, BRAF, and EGFR genes. • Measure gene expression fold changes between samples • Amplicons are designed to be most inclusive for transcripts of the target gene • Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA •...
Catalog: 4482572
Size: 24 reactions

Ion AmpliSeq™ RNA Library Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482340
Size: 96 reactions