Seq Nucleic Acid Analysis Kits

BRAF Mutation Analysis Reagents

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Genetic & Chromosome Abnormality Detection Kits

The BRAF Mutation Analysis Reagents employ a simple fragment analysis workflow to detect 1% mutant to wild type genomic DNA across 3 common and rare mutations in the BRAF gene region (see Table 1). The BRAF set provides sufficient reagent for analysis of 30 samples. Advantages of the BRAF Mutation Analysis Reagents include: • Simple Analysis—unambiguous, easy-to-interpret results • Thorough Coverage—detects 3 mutations in the BRAF gene • Sensitive—detects mutations at frequencies as low as 1 to 5% against a wild type genomic DNA background • Efficient—interrogate multiple loci from the same sa...
Catalog: 4452136
Size: 1 kit

FISH Tag™ DNA Multicolor Kit, Alexa Fluor® dye combination

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Fluorescent Nucleic Acid Detection (FISH) Kits

The FISH Tag™ DNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ DNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. We also offer FISH Tag™ Kits in single colors. FISH Tag™ Kit Specificati...
Catalog: F32951
Size: 1 kit

FISH Tag™ DNA Green Kit, with Alexa Fluor® 488 dye

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Fluorescent Nucleic Acid Detection (FISH) Kits

FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit , which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. FISH Tag™ Kit Specificatio...
Catalog: F32947
Size: 1 kit

MEGAshortscript™ T7 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of large mass amounts of RNA produced from small templates ( • Efficiently transcribes small templates ( • Designed for use with plasmid, synthetic oligonucleotide, or PCR product templates Short transcripts require many more transcription initiation events to synthesize a given mass of product than do longer transcripts. Since the initiation step is the rate-limiting step in transcription reactions, the yield of product from short templates is often low. The MEGAshortscript™ High Yield Tra...
Catalog: AM1354M
Size: 25 rxns w⁄ Manual

MEGAscript® T7 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions. Each Ambion® kit includes sufficient reagents for 25 reactions. • Exclusive, ultrahigh yield technology is fast: 2 hour reaction time • Amplifies aRNA for gene array analysis and other applications • Efficiently incorporates many modified nucleotides A typical 20 µl MEGAscript® reaction with 1 µg of the pTRI-Xef-1alpha control template will yield over 100 µg of transcript. The versatility of the MEGAscript® k...
Catalog: AM1333M
Size: 25 rxns w⁄ Manual

TaqMan® GMO Maize Quantification Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > GMO Detection Kits

The Taqman® GMO Maize Quantification Kit is a food safety testing kit used to determine the number of copies of P35S promoter present in genetically modified organisms compared to the number of copies of maize present in any food or feed sample. The benefits of the Taqman® GMO Maize Quantification Kit over other commercially available products include: • Efficiency—detection of small DNA fragments through use of Taqman® MGB probes • Sensitivity—quantification limit of 20 DNA copies; detection limit of 3 DNA copies (maize and P355 systems) • Flexibility—adapted to processed DNAs The Taqman® GMO...
Catalog: 4481972
Size: 48 reactions

TaqMan® Roundup Ready Soya Quantification Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > GMO Detection Kits

The TaqMan® Roundup Ready Soya Quantification Kit is a food safety testing kit used to determine the percentage of Roundup Ready soya against the total amount of soya present in a DNA sample. This real-time PCR kit contains all reagents (except for the template DNA) required for the quantification of Roundup Ready soya. The benefits of the TaqMan® Roundup Ready Soya Quantification Kit over other commercially available products include: • Efficiency—detection of small DNA fragments through use of Taqman® MGB probes • Sensitivity—quantification limit of 20 DNA copies and detection limit of 3 DNA...
Catalog: 4466335
Size: 2 x 48 reactions

mMESSAGE mMACHINE® T7 Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA; up to 39 µg of RNA per reaction • Extra convenience; cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped...
Catalog: AM1344
Size: 25 reactions

mMESSAGE mMACHINE® T3 Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA—up to 39 µg of RNA per reaction • Extra convenience—cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped R...
Catalog: AM1348
Size: 25 reactions

mMESSAGE mMACHINE® T7 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA; up to 39 µg of RNA per reaction • Extra convenience; cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped...
Catalog: AM1344M
Size: 25 rxns w⁄ Manual

5X mMESSAGE mMACHINE® T7 ULTRA Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

The Ambion® mMESSAGE mMACHINE™ T7 Ultra Kit combines a new cap analog—Anti-Reverse Cap Analog (ARCA)—with Ambion's patented high-yield transcription technology, to generate RNA transcripts that produce higher protein yields compared to other transcripts upon translation. The kit includes sufficient reagents for 50 reactions. • Synthesize more protein from in vitro –transcribed RNA • Express RNA transcripts more efficiently both in vitro and in vivo • Stabilize transcripts in vivo with included reagents for poly(A) tailing ARCA-capped RNA is Translationally More Active: A base modification in A...
Catalog: AMB1345-5
Size: 50 reactions

mMESSAGE mMACHINE® T3 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA—up to 39 µg of RNA per reaction • Extra convenience—cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped R...
Catalog: AM1348M
Size: 25 rxns w⁄ Manual

MEGAscript® T7 Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions. Each Ambion® kit includes sufficient reagents for 25 reactions. • Exclusive, ultrahigh yield technology is fast: 2 hour reaction time • Amplifies aRNA for gene array analysis and other applications • Efficiently incorporates many modified nucleotides A typical 20 µl MEGAscript® reaction with 1 µg of the pTRI-Xef-1alpha control template will yield over 100 µg of transcript. The versatility of the MEGAscript® k...
Catalog: AM1333
Size: 25 reactions

mMESSAGE mMACHINE® SP6 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA • Extra convenience; cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped RNA Transcription will yield lar...
Catalog: AM1340M
Size: 25 rxns w⁄ Manual

TaqMan® GMO Screening Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > GMO Detection Kits

The TaqMan® GMO Screening Kit analyzes DNA extracted from food and feed samples for the presence of the main transgenic regulatory regions. This real-time PCR kit contains all reagents (except for the template DNA) required for the detection of genetically modified organisms (GMOs ). In addition, the kit contains an Internal Positive Control (IPC) to identify possible PCR inhibition. The benefits of the Taqman® GMO Screening Kit over other commercially available products include: • Flexibility—GMOs detected in DNA obtained from ANY food or feed source • Accuracy—an internal positive control (I...
Catalog: 4466334
Size: 4 x 48 reactions

NorthernMax®-Gly 10X Gel Prep/Running Buffer

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Northern Blotting Kits & Reagents

For the preparation and running of agarose gels. For use with RNA denatured by glyoxal treatment; one bottle containing 1 L is provided. Ambion® NorthernMax® reagents are the same components included in the NorthernMax® Ultrasensitive Northern Blotting Kits but in larger sizes. These reagents are supplied ready to use and are certified RNase-free. Comprehensive instructions are included.
Catalog: AM8678
Size: 1 L

NorthernMax® 10X Denaturing Gel Buffer

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Northern Blotting Kits & Reagents

An improved formula for preparing denaturing formaldehyde/MOPS agarose gels. Formaldehyde is included. Gels made with this buffer are considerably less brittle than conventional formaldehyde gels, making handling of the gels much easier. One bottle containing 250 mL is provided. NorthernMax® reagents are the same components included in the NorthernMax® Ultrasensitive Northern Blotting Kits but in larger sizes. These reagents are supplied ready to use and are certified RNase-free. Comprehensive instructions are included.
Catalog: AM8676
Size: 250 ml