sensors & indicators
MitoSOX™ Red reagent is a novel fluorogenic dye specifically targeted to mitochondria in live cells. Oxidation of MitoSOX™ Red reagent by superoxide produces red fluorescence. • Readily oxidized by superoxide but not by other ROS- or RNS-generating systems • Absorption/emission maxima: ~510/580 nm • Use for live cell imaging • Rapidly and selectively targeted to the mitochondria Quick, Easy Detection of Mitochondrial Superoxide in Live Cells The production of superoxide by mitochondria can be visualized in fluorescence microscopy using the MitoSOX™ Red reagent. MitoSOX™ Red reagent permeates l...
Catalog: M36008
Size: 10 × 50 µg
Size: 10 × 50 µg
CM-H 2 DCFDA is a chloromethyl derivative of H 2 DCFDA, useful as an indicator for reactive oxygen species (ROS) in cells. This indicator exhibits much better retention in live cells than H 2 DCFDA. CM-H 2 DCFDA passively diffuses into cells, where its acetate groups are cleaved by intracellular esterases and its thiol-reactive chloromethyl group reacts with intracellular glutathione and other thiols. Subsequent oxidation yields a fluorescent adduct that is trapped inside the cell, thus facilitating long-term studies. ROS Indicator Specifications: • Ex/Em: ~492–495/517–527 nm • Product is air ...
Catalog: C6827
Size: 20 × 50 µg
Size: 20 × 50 µg
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+ . Fluo-3 has been used to image the spatial dynamics of Ca 2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt ...
Catalog: F-14200
Size: 500 µg
Size: 500 µg
The Calcium Calibration Buffer Kits are designed to help determine the dissociation constant (K d ) of fluorescent Ca 2+ indicators using a fluorometer or quantitative imaging system. The Kd of an indicator can be calculated from a plot generated by scanning the excitation or emission of the indicator in the presence of eleven different Ca 2+ concentrations. Calcium Calibration Kit #1 contains 50 mL of 10 mM K 2 EGTA and 50 mL of 10 mM CaEGTA. Both solutions contain 100 mM KCl and 30 mM MOPS, pH 7.2 and are prepared in deionized water. These stock solutions can be blended to make buffers havin...
Catalog: C-3008MP
Size: 1 kit
Size: 1 kit
Intracellular calibration of the fluorescence response of cytosolic pH indicators is typically performed using the K + /H + ionophore nigericin , which causes equilibration of intracellular and extracellular pH in the presence of a depolarizing concentration of extracellular K + .
Catalog: N-1495
Size: 10 mg
Size: 10 mg
Voltage Sensor Probes (VSPs) are a Fluorescence Resonance Energy Transfer (FRET)-based voltage-sensing assay technology used to measure changes in cellular membrane electrical potential. VSPs are ideal for screening compounds that modulate ion channels because they provide: High-throughput capabilities-screen 32,000 samples in one day Highly sensitive detection-accurately and precisely report a 1% change in the Emission Ratio per mV Rapid detection response-sub-second time response Ratiometric readout (the Emission Ratio)-reduces experimental errors arising from well-to-well variations in cell...
Catalog: K1022
Size: 5 mg
Size: 5 mg
Our highly purified lucigenin is a fluorescent Cl- indicator that has excitation/emission maxima of 368/505 nm and whose fluorescence is quenched in the presence of Cl-. The dye is useful for determining Cl- concentrations in liposomes, reconstituted membranes or in extracellular medium but is not useful for intracellular measurements.
Catalog: L6868
Size: 10 mg
Size: 10 mg
Fura-FF pentapotassium salt is a fura-2 derivative that has Ca 2+ sensitivity similar to mag-fura-2, but is relatively insensitive to Mg 2+ . This water-soluble salt form is useful for intracellular loading by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Catalog: F14180
Size: 500 µg
Size: 500 µg
Carboxy-DCFDA can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pK a of 4.8, this dye can be used as an acidic pH sensor.
Catalog: C-369
Size: 100 mg
Size: 100 mg
To permit simultaneous long-term tracing of mixed-cell populations using different fluorescent colors, we have developed a probe whose applications should be similar to those of CFDA SE (commonly called CFSE). Cells labeled with our SNARF-1 carboxylic acid, acetate, succinimidyl ester have orange-red fluorescence that can be easily distinguished from that of cells loaded with green-fluorescent tracers such as CFDA SE.
Catalog: S22801
Size: 10 × 50 µg
Size: 10 × 50 µg
OxyBURST® Green H 2 DCFDA succinimidyl ester (2',7'-dichlorodihydrofluorescein diacetate, SE) is an amine-reactive assay reagent that can be used to prepare oxidation-sensitive conjugates of a wide variety of biomolecules and particles, including antibodies, antigens, peptides, proteins, dextrans, bacteria, yeast and polystyrene microspheres. Following conjugation to amines, the two acetates of OxyBURST® Green H 2 DCFDA can be removed by treatment with hydroxylamine at neutral pH to yield the dihydrofluorescein conjugate. OxyBURST® Green H 2 DCFDA conjugates are nonfluorescent until they are o...
Catalog: D-2935
Size: 5 mg
Size: 5 mg
SNAP spontaneously releases nitric oxide (NO) under physiological conditions.
Catalog: N-7892
Size: 25 mg
Size: 25 mg
DAF-FM is a reagent that is used to detect and quantify low concentrations of nitric oxide (NO). It is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fluorometers. DAF-FM Specifications: • Ex/Em of DAF-FM: ~495/515 nm • Lyophilized product should be dissolved using DMSO and then added to an aqueous buffer to create a working solution • DAF-FM diacetate is cell permeant and passively diffuses across...
Catalog: D-23844
Size: 10 × 50 µg
Size: 10 × 50 µg
MTT is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble MTT to an insoluble purple formazan. The formazan is then solubilized and its concentration determined by optical density.
Catalog: M-6494
Size: 1 g
Size: 1 g
sensors & indicators
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