Secondary Detection Kits & Reagents

Protein A-HRP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Detection Kits & Reagents > Protein A

Protein A from Staphylococcus aureus, Cowan I Strain conjugated with highly purified horseradish peroxidase (RZ > 3.0). The product is liquid containing 10 mM phosphate, 0.15 M NaCl buffer, pH 7.4, 1% ovalbumin, 40% glycerol, and 0.1% proclin as preservative.
Catalog: 10-1023
Size: 3 mg

Recombinant Protein G-HRP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Detection Kits & Reagents > Protein G

Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This protein G is conjugated with highly purified horseradish peroxidase (RZ > 3.0) and is in enyme conjugate stabilizer containing 0.1% proclin as preservative. Recombinant protein G binds to the Fc region of many types of immunoglobulin G including: all four subclasses of human IgG mouse IgG rabbit IgG guinea pig IgG rat IgG goat IgG sheep IgG cow IgG horse IgG Recombinant protein G does not bind to IgG from chicken or cat, or to human IgA, IgM, or serum albumin. It binds wea...
Catalog: 10-1223
Size: 1 ml

Zenon® Pacific Green™ Mouse IgG1 Labeling Kit

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Antibody Labeling Kits (Zenon® Secondary Detection-Based)

Zenon® labeling technology provides a fast, versatile, and reliable method for adding a fluorescent label to an antibody. You need only a small amount of starting material,and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Antibody conjugates formed using Zenon® technology may be used in protocols where a directly labeled primary antibody is suitable, including flow cytometry, imaging, and high-throughput applications. This exclusive Molecular Probes® Zenon® labeling technology greatly simplifies the use of multiple mouse-derived...
Catalog: Z11203
Size: 1 kit

BRAF Mutation Analysis Reagents

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Genetic & Chromosome Abnormality Detection Kits

The BRAF Mutation Analysis Reagents employ a simple fragment analysis workflow to detect 1% mutant to wild type genomic DNA across 3 common and rare mutations in the BRAF gene region (see Table 1). The BRAF set provides sufficient reagent for analysis of 30 samples. Advantages of the BRAF Mutation Analysis Reagents include: • Simple Analysis—unambiguous, easy-to-interpret results • Thorough Coverage—detects 3 mutations in the BRAF gene • Sensitive—detects mutations at frequencies as low as 1 to 5% against a wild type genomic DNA background • Efficient—interrogate multiple loci from the same sa...
Catalog: 4452136
Size: 1 kit

DTNB; Ellman's Reagent, 5,5'-Dithiobis-(2-Nitrobenzoic Acid)

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Thiol-Reactive Fluorophores, Biotins & Other Labels

DTNB or Elman's reagent can be used to quantitate thiols in proteins, cells and plasma by absorption measurements. It readily forms a mixed disulfide with thiols, liberating the chromophore 5-merapto-2-nitrobenzoic acid (absorption maximum 410 nm). Only protein thiols that are accessible to this water-soluble reagent are modified.
Catalog: D-8451
Size: 10 g

MitoProbe™ DiIC 1 (5) Assay Kit - For Flow Cytometry - 100 Assays

Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents

The MitoProbe™ DiIC 1 (5) Assay Kit provides solutions of the membrane-potential-sensitive cyanine dye DiIC 1 (5), and a mitochondrial membrane-potential disrupter, CCCP, for the study of mitochondrial membrane potential. DiIC 1 (5) penetrates the cytosol of eukaryotic cells and accumulates primarily in mitochondria with active membrane potentials, producing bright, far-red fluorescence. DiIC 1 (5) staining intensity decreases with decreased mitochondrial membrane potential.
Catalog: M34151
Size: 1 kit

Goat Anti-Mouse IgG (H+L) - AP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Immunogen: Mouse IgG (H+L) Specificity: Mouse IgG (H+L) Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB ZyMAX™ antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX™ conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background. Enhanced sensitivity,...
Catalog: 62-6522
Size: 1 ml

Mouse Anti-Human IgG

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Clone/PAD: HP6045 Immunogen: Human IgG Specificity: This monoclonal antibody reacts with human IgG heavy chains (Fd region) of all subclasses. Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, Western blotting
Catalog: 05-4500
Size: 0.5 mg

Goat Anti Human IgG HRP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Goat whole molecule anti-human immunoglobulin Format/Formulation: HRP Conjugate
Catalog: AHI0304
Size: 0.7 mg⁄ml

Goat Anti-Human IgG, Biotin

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Goat whole molecule anti-human immunoglobulin Format/Formulation: Biotin Conjugate
Catalog: AHI0309
Size: 0.7 mg⁄ml

Alexa Fluor® 488 Rabbit Anti-Rat IgG (H+L)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Alexa Fluor 488 rabbit anti—rat IgG is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rat. To minimize crossreactivity, it has been adsorbed against human IgG prior to conjugation.
Catalog: A-21210
Size: 0.5 ml

F(ab')2 Fragment of Goat Anti-Rat IgG (H+L), Horseradish Peroxidase Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Affinity purified F(ab')2 gragment of goat anti-rat IgG conjugated to horseradish peroxidase. To minimize cross-reactivity, this antibody has been absorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum.
Catalog: A10548
Size: 0.5 mg

Alexa Fluor® 488 Goat Anti-Mouse IgG 1 (γ1)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

The Alexa Fluor® 488 goat anti-mouse IgG 1 is labeled with our exceptionally bright, photostable Alexa Fluor® 488 dye (which is spectrally similar to fluorescein) and is prepared from affinity-purified antibodies that react with the Fc portion of the heavy chain of mouse IgG 1 . To minimize crossreactivity, the antibody has been adsorbed against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins and mouse isotypes IgG 2a , IgG 2b and IgG 3 prior to labeling.
Catalog: A-21121
Size: 250 µl

ZyMAX™ Rabbit Anti-Sheep IgG (H+L) - FITC (ZyMAX™ Grade)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Immunogen: Sheep IgG (H+L) Specificity: Sheep IgG (H+L) Applications: Flow cytometry, Immunofluorescence ZyMAX™ antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX™ conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background. Enhanced sensitivity, higher titer, lower background -- try them t...
Catalog: 81-8611
Size: 1.5 mg

Goat Anti-Human IgM, HRP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Goat whole molecule anti-human immunoglobulin Format/Formulation: HRP Conjugate
Catalog: AHI0604
Size: 0.7 mg⁄ml

Alexa Fluor® 633 Donkey Anti-Goat IgG (H+L)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

The Alexa Fluor 633 donkey anti—goat IgG is labeled with our bright, far-red—fluorescent Alexa Fluor 633 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from goat. To minimize crossreactivity, it has been adsorbed against rabbit, rat, mouse and human IgG. Fluorescence of this long-wavelength Alexa Fluor dye is not visible to the human eye but is readily detected by most imaging systems.
Catalog: A-21082
Size: 0.5 ml

Alexa Fluor® 594 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

This highly cross-adsorbed Alexa Fluor® 594 Goat Anti-Rabbit IgG (H+L) is labeled with our superior red-fluorescent Alexa Fluor 594 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize cross-reactivity, it has been adsorbed against bovine IgG, goat IgG, mouse IgG, rat IgG and human IgG.
Catalog: A-11037
Size: 0.5 ml

Texas Red®-X Goat Anti-Rabbit IgG (H+L)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

The Texas Red-X goat anti—rabbit IgG is labeled with our bright, photostable, red-fluorescent dye (which contains an additional seven-atom aminohexanoyl spacer ("X") between the fluorophore and the antibody). The antibody is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize crossreactivity, it has been adsorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum.
Catalog: T-6391
Size: 0.5 ml

NorthernMax®-Gly 10X Gel Prep/Running Buffer

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Northern Blotting Kits & Reagents

For the preparation and running of agarose gels. For use with RNA denatured by glyoxal treatment; one bottle containing 1 L is provided. Ambion® NorthernMax® reagents are the same components included in the NorthernMax® Ultrasensitive Northern Blotting Kits but in larger sizes. These reagents are supplied ready to use and are certified RNase-free. Comprehensive instructions are included.
Catalog: AM8678
Size: 1 L

Rabbit Anti-Goat IgG (H+L), Horseradish Peroxidase Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

The rabbit anti—goat IgG, horseradish peroxidase (HRP) conjugate has been adsorbed against human and rat serum proteins prior to labeling to minimize crossreactivity.
Catalog: R-21459
Size: 1 mg