Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents
Cell extraction buffer suitable for use in ELISA and Western blotting.
Catalog: FNN0011
Size: 100 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers
NuPAGE® LDS Sample Buffer (4X) (10 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent. For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X) . Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sample...
Catalog: NP0007
Size: 10 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers
NuPAGE® LDS Sample Buffer (4X) (250 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent. For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X) . Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sampl...
Catalog: NP0008
Size: 250 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers
Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recomme...
Catalog: LC2676
Size: 20 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers
NativePAGE™ 5% G-250 Sample Additive is added to samples prepared with the NativePAGE™ Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE™ gels. This Additive is also included in the NativePAGE™ Sample Prep Kit. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.
Catalog: BN2004
Size: 0.5 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents
The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step. PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous ...
Catalog: 4318930
Size: 1 kit
Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents
The Protein Quant Sample Lysis Kit contains the reagents required for the preparation of target and control cell lysates for use with TaqMan® Protein Assay experiments. It contains a one-step lysis solution for preparing crude cell lysates from cultured cells, and the lysates are compatible with cell counting as well as total protein quant measurements such as a microBCA assay. The Protein Quant Sample Lysis Kit contains a buffered nonionic detergent solution for the gentle lysis of adherent and non-adherent cultured cells under non-denaturing conditions
Catalog: 4448536
Size: 25 ml
Category: Products & Services > Kits & Assays > Impurity & Contamination Detection Kits > Host Cell DNA & Protein Impurity Quantitation Kits
The resDNASEQ® Pichia pastoris Residual DNA Quantitation System is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Pichia pastoris cells, an expression system commonly used for the production of recombinant proteins. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 15 pg DNA/mL test sample) and specific quantitation of Pichia pastoris cell DNA, typically in less than four hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—w...
Catalog: 4464338
Size: 100 reactions
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Other Instrument Accessories
The ParaDNA® Sample Collector is a part of the ParaDNA® Screening System. With minimal upfront training required, the ParaDNA® Screening System enables law enforcement and forensic practitioners to screen a small portion of their crime scene samples in order to obtain DNA-level information. The system provides the user with objective sample information estimating the quality and quantity of the DNA, as well as the gender of the sample’s contributor. The information generated can allow for better case prioritization, support decisions relating to an agency’s sample submission policy and enable ...
Catalog: 4484203
Size: 8 pack
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers
Denaturing polyacrylamide TBE-Urea Gels resolve single-stranded DNA oligos or RNA into sharp, distinct bands. Novex® TBE-Urea Gels are optimized for the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies, and Northern blot analysis. Novex® TBE-Urea Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE-Urea Gels are made with high-purity reagents, including tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEME...
Catalog: LC6876
Size: 10 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers
Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex® DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex® TBE and DNA Retardation Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide,...
Catalog: LC6678
Size: 10 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Genomic DNA Purification Kits
MagMAX™ DNA Multi-Sample 96-Well Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells. These purification kits use MagMAX™ magnetic bead-based nucleic acid isolation technology to produce high yields of purified DNA, free from inhibitors that may affect downstream reactions such as genotyping assays, CE and next-generation sequencing, and high resolution melting analysis. The 96-prep and 5x 96-prep kits are designed for higher throughput and automat...
Catalog: 4413022
Size: 5 x 96 preps
Category: Products & Services > Buffers & Chemicals > Reducing Agents
NuPAGE® Reducing Agent (10X) (10 ml) is used to reduce protein samples for protein gel electrophoresis. NuPAGE® Reducing Agent contains 500 mM dithiothreitol (DTT) at a ready-to-use 10X concentration in a stabilized liquid form. Use NuPAGE® Sample Reducing Agent when running proteins on NuPAGE® Bis-Tris gels to maintain protein samples in a reduced state during electrophoresis. For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
Catalog: NP0009
Size: 10 ml
Category: Products & Services > Kits & Assays > Impurity & Contamination Detection Kits > Host Cell DNA & Protein Impurity Quantitation Kits
The resDNASEQ® MDCK Residual DNA Quantitation System is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of MDCK cell DNA, typically in less than 4 hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high...
Catalog: 4464337
Size: 100 reactions
Category: Products & Services > Kits & Assays > Impurity & Contamination Detection Kits > Host Cell DNA & Protein Impurity Quantitation Kits
The resDNASEQ® NS0 Residual DNA Quantitation System is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from NS0 (murine myeloma) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of NS0 cell DNA, typically in less than 4 hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high m...
Catalog: 4460364
Size: 100 reactions
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers
Our Magnetic Particle Concentrators (MPC®) enable optimal binding of Dynabeads® magnetic beads with their targets (e.g., cells, proteins, and nucleic acids) in a variety of starting samples. The MPC®-S was designed specifically for microbiology applications; it has been validated with select Dynabeads® for enrichment of E. coli O157:H7, Salmonella, non-O157 STEC, Cryptosporidium, and Giardia. The MPC®-S can be used with both food enrichment samples types as well as water samples. It holds up to six microcentrifuge tubes and has an optimal working volume range of 20 µl–2 ml. Note that this magn...
Catalog: A13346
Size: 1 unit
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers
Magnetic Stand is part of the I-SAGE kit
Catalog: R670-01
Size: each
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers
The Magnetic 96-Well Separator is designed for fast and simple removal of supernatant from samples bound to magnetic particles and may be used manually or as an integrated part of an automated workstation. The Magnetic 96-Well Separator is an ideal tool for use in singleplex and multiplex biological assays based on the Luminex® xMAP® Technology. These assays make use of Luminex® MagPlex™, or magnetic microspheres, and the MAGPIX®, Luminex® 200™ or FLEXMAP 3D® instruments. Note: It is recommended that the Magnetic 96-Well Separator be used with a standard 96-well flat-bottom plate such as Grein...
Catalog: A14179
Size: 1 each
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing
The Pippin Prep™ System is a small tabletop instrument that automates the size selection and collection of DNA for PGM™ sequencer libraries. Simply enter the desired base-pair target range via the software interface and after a 1.5 hr run the system will yield the programmed DNA fractions with virtually no low molecular weight contamination. The tight fragment size distributions obtained provide a more uniform template for amplification. And the processing of samples is simplified through the use of disposable pre-cast agarose gel cassettes. Benefits of the Pippin Prep™ System • Time savings c...
Catalog: 4471271
Size: 1 each
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers
The DynaMag™-5 Magnet is intended for use with Dynabeads® for the immunomagnetic separation of cells. The DynaMag™-5 magnet accommodates tubes from 11–16 mm in diameter (e.g., flow cytometry tubes and most blood collection tubes), with an optimal working volume of 1–5 mL. The magnet holds 8 tubes and is compatible with all Dynabeads® (1–4.5 µM in diameter). When performing cell separation using Dynabeads®, a DynaMag™ magnet is required. Different magnets are optimized for different working volumes and tube sizes. See our complete overview of the DynaMag™ magnets . The DynaMag™-5 magnet contain...
Catalog: 12303D
Size: 8 samples