Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Running Buffers
The NativePAGE™ Running Buffer Kit contains the NativePAGE™ Running Buffer (20X), which is used to make the NativePAGE™ Cathode and Anode Running Buffers for use with an XCell SureLock® Mini Cell when running NativePAGE™ Gels. The kit also contains the NativePAGE™ Cathode Buffer Additive (20X) that is used with the NativePAGE™ Running Buffer to make the Cathode Running Buffer for the system.
Catalog: BN2007
Size: 1 kit
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Running Buffers
The NuPAGE® MOPS SDS Running Buffer (20X) is formulated for running proteins on NuPAGE® Novex® Bis-Tris gels. NuPAGE® MOPS SDS Running Buffer is recommended for separating medium- to large-sized proteins. Use the Right Buffer to Optimize Protein Separations NuPAGE® MES SDS Running Buffer and NuPAGE® MOPS SDS Running Buffer can both be used with the NuPAGE® Bis-Tris gels. Using MOPS buffer allows proteins to run slower vs. MES buffer. Get Consistent, Convenient High-Quality Results Pre-mixed NuPAGE® buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buf...
Catalog: NP0001-02
Size: 5 L
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Running Buffers
NuPAGE® MES SDS Running Buffer (20X) is formulated for running proteins on NuPAGE® Novex® Bis-Tris gels. NuPAGE® MES SDS Running Buffer is recommended for separating small- to medium-sized proteins. Use the Right Buffer to Optimize Protein Separations NuPAGE® MES SDS Running Buffer and NuPAGE® MOPS SDS Running Buffer can both be used with the NuPAGE® Bis-Tris gels. Using MES buffer allows proteins to run faster vs. MOPS buffer. Get Consistent, Convenient High-Quality Results Pre-mixed NuPAGE® buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buffers a...
Catalog: NP0002
Size: 500 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Running Buffers
NuPAGE® Novex Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended Buffers: Run NuPAGE® Novex Tris-Acetate gels...
Catalog: LA0041
Size: 500 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Running Buffers
Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recomme...
Catalog: LC2675-5
Size: 5 L
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Running Buffers
UltraPure™ DNA Typing Grade® 50X TAE Buffer is a sterile-filtered solution of 2 M Tris-acetate and 50 mM EDTA. TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. To prepare a 1X solution, mix one volume of UltraPure™ 50X TAE Buffer with 49 volumes of distilled water. A 1X TAE Buffer solution contains 40 mM Tris-acetate and 1 mM EDTA with a pH of 8.3 at 23“C. Performance and Quality Testing: No DNase activity detected.
Catalog: 24710-030
Size: 1 L
Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Northern Blotting Kits & Reagents
For the preparation and running of agarose gels. For use with RNA denatured by glyoxal treatment; one bottle containing 1 L is provided. Ambion® NorthernMax® reagents are the same components included in the NorthernMax® Ultrasensitive Northern Blotting Kits but in larger sizes. These reagents are supplied ready to use and are certified RNase-free. Comprehensive instructions are included.
Catalog: AM8678
Size: 1 L
Category: Products & Services > Buffers & Chemicals > Molecular Biology Enzyme Buffers > PCR Enzyme Buffers
Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 ml of 50 mM MgCl 2 . It is included with Platinum® Taq, Taq, and the SuperScript® First-Strand Synthesis System for RT-PCR.
Catalog: 18067-017
Size: 2 × 1 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Agarose
DNA Grade Agarose has a high sieve capacity, providing optimal resolution of DNA during gel electrophoresis. The low sulfur content allows gels to be run faster without becoming brittle. Supplied as a powder that is reconstituted in 0.5X TBE for high-quality agarose gels.
Catalog: 75000-500
Size: 500 g
Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > Nuclease Control Reagents
Ambion® DNA Zap ™ (patent pending) consists of two solutions that are innocuous when used alone, but become a potent nucleic acid degrading solution when mixed. This mixture is able to instantaneously degrade high levels of contaminating DNA and RNA from surfaces. 250 mL of each solution are provided. • Completely degrades contaminating DNA and RNA at the level of PCR sensitivity • Works on contact • Degrades nucleic acid, unlike other products on the market that only act as detergents • Ideal for cleaning PCR tubes, PCR machine surfaces, pipettors, lab benches, lab equipment, and microfuge tu...
Catalog: AM9890
Size: 250 ml
Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > Nuclease Control Reagents
Ambion® DNA Zap ™ (patent pending) consists of two solutions that are innocuous when used alone, but become a potent nucleic acid degrading solution when mixed. This mixture is able to instantaneously degrade high levels of contaminating DNA and RNA from surfaces. 250 mL of each solution are provided. • Completely degrades contaminating DNA and RNA at the level of PCR sensitivity • Works on contact • Degrades nucleic acid, unlike other products on the market that only act as detergents • Ideal for cleaning PCR tubes, PCR machine surfaces, pipettors, lab benches, lab equipment, and microfuge tu...
Catalog: AM9890M
Size: 250 ml (Manual)
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Running Buffers
Molecular biology grade, Ambion® 10X TBE solution is supplied in four bottles containing 1 L each. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating...
Catalog: AM9865
Size: 4 bottle
Category: Products & Services > Buffers & Chemicals > General Chemicals > Oligo Synthesis Reagents
Contains 45 mg 6-FAM™ Dye Phosphoramidite. 6-FAM™ Dye Phosphoramidite is used for the 5’ attachment of FAM™ dye during oligonucleotide synthesis. 6-FAM™ Dye Phosphoramidite is an optically pure isomer (not a mixture). The label is treated as if it were simply another base: It is inserted at the appropriate place in the sequence and incorporated automatically. • Specialty phosphoramidites allow online addition of the label during synthesis, saving time in production of labeled probes and primers • Designed and optimized for use with ABI™ synthesizers, guaranteeing reliable nucleic acid synthesi...
Catalog: 403169
Size: 45 mg
Category: Products & Services > Buffers & Chemicals > General Chemicals > Oligo Synthesis Reagents
Contains 50 mg TET™ Dye Phosphoramidite. TET™ Dye Phosphoramidite is used for the 5’ attachment of TET™ dye during oligonucleotide synthesis. The label is treated as if it were simply another base: It is inserted at the appropriate place in the sequence and incorporated automatically. • Specialty phosphoramidites allow online addition of the label during synthesis, saving time in production of labeled probes and primers • Designed and optimized for use with ABI™ synthesizers, guaranteeing reliable nucleic acid synthesis • Life Technologies offers a range of specialty phosphoramites and linkers...
Catalog: 403171
Size: 50 mg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits
Applied Biosystems® AmpliTaq® DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus ( Taq ) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot. AmpliTaq® DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy Its thermal activity profile makes it good ...
Catalog: N8080160
Size: 250 units
Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > DNA & RNA Storage Reagents
RNAlater®-ICE is a novel reagent for transitioning frozen tissue to a state that is easily processed for extraction of high quality RNA. Frozen tissues are simply submerged in RNAlater®-ICE and allowed to thaw overnight at -20°C. Once thawed, the tissues can be processed like fresh tissues using standard RNA isolation procedures. No more laborious grinding of frozen tissue to safeguard the RNA. • Process previously frozen tissues like freshly harvested samples • Thawing tissues in RNAlater®-ICE protects RNA from degradation • No more tissue pulverizing with mortar and pestle and awkward transf...
Catalog: 4427575
Size: 10 bottles
Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > DNA & RNA Storage Reagents
THE Ambion® RNA Storage Solution is a buffer that provides greater RNA stability than standard 0.1 mM EDTA or TE Buffer. This 1 mM sodium citrate, pH 6.4 +/- 0.2 buffer is provided in one bottle containing 50 mL. The last step in every RNA isolation protocol is to resuspend the purified RNA pellet. After painstakingly isolating the RNA, it is crucial that the pellet be suspended and stored in a safe, RNase-free solution. THE RNA Storage Solution has two features that minimize base hydrolysis of RNA: a low pH, and sodium citrate, which is an efficient chelating agent. THE RNA Storage Solution i...
Catalog: AM7001
Size: 50 ml