Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits
The SuperScript® III First-Strand Synthesis System for RT-PCR provides all the necessary components for synthesis of high-quality first-strand cDNA from total or poly(A)+ RNA. Used with PCR, this functionally tested system enables you to detect rare messages and generate enough material for cloning. The SuperScript® III First-Strand Synthesis System: Uses SuperScript® III RT for higher cDNA yields (Figure 1) Includes all components needed for first-strand cDNA synthesis Consists of optimized reagents for more robust performance Contains a streamlined protocol Detects template molecules from as...
Catalog: 18080-051
Size: 50 reactions
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Reverse Transcriptase
SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A) + RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity...
Catalog: 18080-044
Size: 10,000 units
Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits
The SuperScript® III First-Strand Synthesis SuperMix provides high yields of first-strand cDNA in a convenient high-throughput supermix format. The simple, time-saving reaction set-up uses just two tubes-a 2X Reaction Mix and a SuperScript® III Enzyme Mix (Figure 1). An optional annealing buffer is provided for improved yields and sensitivity. The Enzyme Mix includes SuperScript® III Reverse Transcriptase to ensure higher cDNA yields, superior sensitivity-down to 0.1 pg (Figure 2)-and specificity, and compatibility with a wide variety of RT-PCR applications.
Catalog: 18080-400
Size: 50 reactions
Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > DNA Ladders
Ambion® Lambda DNA is digested to completion with Hind III. This generates fragments of the sizes 23130, 9416, 6557, 4361, 2322, 2027, 564, and 125 bp. These DNA molecular mass markers are supplied with a protocol describing easy labeling of the markers with radioisotopes. They can also be stained with ethidium bromide. One tube containing 200 μg is provided.
Catalog: AM7720
Size: 200 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
This is a purified rabbit anti-human Carbonic Anhydrase III Polyclonal Antibody, that can be used in the validated applications of Western blot, ELISA, and immunohistochemistry. This Carbonic Anhydrase III Polyclonal Antibody was produced in rabbits immunized with purified, human cell-derived, recombinant human Carbonic Anhydrase III. Carbonic Anhydrase III specific IgG was purified by human Carbonic Anhydrase III affinity chromatograpy. Antibody characteristics include: Applications: This Carbonic Anhydrase III Polyclonal Antibody, has been validated for use in Western blot, ELISA, and immuno...
Catalog: 10503-RP02-AB
Size: 100 µg
Category: Products & Services > Columns, Resins & Plates > Chromatographic Columns & Media > Affinity Media & Standards
This is one of a group of CaptureSelect™ protein purification affinity resins developed for the simple, single-step purification of a variety human plasma and serum proteins. This unique selection of affinity purification resins can purify proteins or other non-antibody biomolecules from either recombinant or native sources with a high degree of affinity and specificity, and are immobilized on a high capacity agarose support.
Catalog: 190317005
Size: 5 ml
Category: Products & Services > Columns, Resins & Plates > Chromatographic Columns & Media > Affinity Media & Standards
This is one of a group of CaptureSelect™ protein purification affinity resins developed for the simple, single-step purification of a variety human plasma and serum proteins. This unique selection of affinity purification resins can purify proteins or other non-antibody biomolecules from either recombinant or native sources with a high degree of affinity and specificity, and are immobilized on a high capacity agarose support.
Catalog: 190317010
Size: 10 ml
Category: Products & Services > Columns, Resins & Plates > Chromatographic Columns & Media > Affinity Media & Standards
This is one of a group of CaptureSelect™ protein purification affinity resins developed for the simple, single-step purification of a variety human plasma and serum proteins. This unique selection of affinity purification resins can purify proteins or other non-antibody biomolecules from either recombinant or native sources with a high degree of affinity and specificity, and are immobilized on a high capacity agarose support.
Catalog: 190317050
Size: 50 ml