Human TaqMan® Copy Number Reference Assays are run with human TaqMan® Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome. Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT. TaqMan® Copy Number Reference Assays are designed to unique genomic sequences in the reference genome assembly (NCBI Build 37/hg19) and are required for relative quantitation of copy number targets. TaqMan® Copy Number Assays quantitate the gene of interest and normalize t...
Size: 750 reactions
The RNase P gene is a single-copy gene that encodes the RNA moiety for the RNase P enzyme. Applied Biosystems® TaqMan® RNase P Control Reagents were designed with limiting primer concentrations to be used as the endogenous reference in multiplex reactions. Multiplex PCR is the use of more than one probe and primer pair in the same tube. This kit is designed to perform a 5' nuclease assay with TaqMan® Universal PCR Master Mix using the provided human genomic DNA as template. • Pre-designed primers and TaqMan® probe eliminate assay design • Easy-to-use primer and TaqMan® probe mix minimizes assa...
Size: 1000 reactions
Ambion® RNaseZap® is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap® onto the surface to be decontaminated and rinse it off with RNase-free water. Supplied in a single 4 liter cubitainer. • Completely removes RNase contamination from glass and plastic surfaces • Excels at removing high levels of RNase contamination whereas similar products fail • Proven effective at removing high concentrations of dried-on RNase A • Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free Working with RNA requires that special measures be...
Size: 4 L
RNase AWAY® Reagent is a ready-to-use solution for eliminating RNase and DNA contamination from labware. Apply it evenly over the surface of glassware or plasticware to be treated and then rinse it away with distilled water. Unwanted RNase and DNA contamination are eliminated. RNase AWAY® Reagent is nonabrasive, noncarcinogenic, and nonbiologically corrosive. Performance and Quality Testing: RNase AWAY® Reagent is functionally tested for the elimination of RNase activity and DNA. No detectable RNase activity or DNA is observed.
Size: 250 ml
The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of input total RNA in a single 20 µL reaction to single stranded cDNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Includes sufficient reagents for 1000 x 20 µL reactions. • Linear target amplification for real-time PCR • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost • 10-fold greater dynamic range than other kits cDNA from Total RNA The High Capacity cDNA Reverse Transcription Kit (for...
Size: 1000 reactions
Optimized for researchers performing RNA structure, RNA sequencing, protein footprinting, and boundary experiments, Ambion® RNase V1, RNA-Grade cleaves base-paired nucleotides and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites. Supplied in one tube of 200 µl (0.1 U/ µL); optimization reagents also included. In addition to applications for RNA structural analysis, RNA-Grade ribonucleases can be used to map protein binding sites on RNAs by comparing cleavage patterns in the presence and absence of an RNA binding p...
Size: 200 µl
The RNase-free TE buffer concentrate, 20X TE buffer , which is essential to the success of the RiboGreen® RNA quantitation assays (R11491, R37302) is available separately from the kits to extend the number of low-concentration assays.
Size: 100 ml
The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7300 and 7500 Real-Time PCR Systems. The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is a 96-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. T...
Size: 1 kit
Ambion® RNase Cocktail™ is a mixture of two highly purified ribonucleases, RNase A (500 U/mL) and RNase T1 (20,000 U/mL), and is free of DNase and nicking activities. Use RNase Cocktail™ for all situations where it is desirable to degrade RNA, i.e. plasmid minipreps and ribonuclease protection assays. Supplied in five tubes containing 1 mL each. Digestion of RNA with RNase A alone leaves fragments of RNA that are large enough to be visible on agarose gels and precipitate in ethanol. RNase A cuts after C and U residues, and RNase T1 cuts after G residues. Consequently, the mixture of both enzym...
Size: 5 tubes
Ambion® RNase-free Microfuge Tubes are guaranteed RNase- and DNase-free and have a positive seal design that withstands –90° to 120°C. They are marked with convenient graduations. 1.5 mL tubes are provided in a box of 500. Each lot of tubes is certified RNAse- and DNase-free.
Size: 500 tubes
Application/s In the isolation of DNA for RNA-free DNA. Concentration 20 mg RNase A/ml in 50 mM Tris-HCl (pH 8.0), 10 mM EDTA Specification/s An essentially protease free, freeze dried powder with an activity of not less than 70 U/mg material Source of product Bovine pancreas EC No. 188.8.131.52
Size: 25 ml
Ambion® Non-Stick RNase-free Microfuge Tubes have a low binding surface and are guaranteed RNase- and DNase-free. They have a positive seal design that withstands –90° to 120°C and are marked with convenient graduations. 1.5 mL tubes are provided in a box of 250. Each lot of tubes is certified RNAse- and DNase-free.
Size: 250 tubes
FxCycle™ PI/RNase Staining Solution is used for flow cyotmetric analysis of DNA content in fixed cells. Propidium iodide (PI), a popular red-fluorescent stain, binds to DNA by intercalating between the bases with little or no sequence preference and with a stoichiometry of one dye per 4–5 base pairs of DNA. PI also binds to RNA, necessitating treatment with RNase to distinguish between RNA and DNA staining. This propidium iodide solution includes DNase-free RNase A and a permeablization reagent. It comes ready to use: simply add the staining solution to fixed cells, incubate, and acquire on a ...
Size: 100 ml
UltraPure™ DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. It is 0.1-µm membrane-filtered and tested for DNase, RNase, and protease activity.
Size: 500 ml