Rna Synthesis
The High Capacity RNA-to-cDNA™ Kit is a streamlined reverse transcription kit designed for optimum performance with the TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix, and other PCR enzymes. Features of the High Capacity RNA-to-cDNA™ Kit: • Fast —Short reaction time (typically 30–60 min) • Convenient —Easy workflow with few pipetting steps (2 tubes) • Reliable —Reliable reverse transcription of both abundant and limited targets • Flexible —Optimized 2-step protocol enabling multiple PCR reactions from a single reverse transcription reaction Streamline Your cDNA Synthesis ...
Catalog: 4387406
Size: 50 reactions
Size: 50 reactions
SuperScript® First-Strand Synthesis System for RT-PCR is optimized for sensitive RT-PCR from low-input RNA, Taq or Platinum® Taq DNA Polymerase for higher specificity PCR or with AccuPrime™ Pfx DNA Polymerase for high-fidelity cloning applications. The SuperScript® First-Strand Synthesis System for RT-PCR: Is optimized for RT-PCR Uses SuperScript® II RT for greater first-strand cDNA yields Detects as little as 50 to 100 molecules of RNA template from only 1 pg of total RNA (Figure 1) Includes oligo(dT) and random hexamers to prime cDNA synthesis for flexible use Amplifies first-strand cDNA pro...
Catalog: 11904-018
Size: 50 reactions
Size: 50 reactions
This Applied Biosystems® product is designed for the purification of RNA from whole blood for real-time RT-PCR, microarrays, or other high-quality RNA experiments. 12 samples can be processed in about 45 minutes. For use with the ABI PRISM® 6100 Nucleic Acid PrepStation, sufficient reagents are included for 24 purifications. • Extract pure, intact RNA • Isolate more than 6 µg of RNA from 3 mL blood • Simple workflow—less time and fewer steps than other kits (see Figure) • Highly stable RNA, free of contaminating gDNA, and inhibitors; no DNase treatment required • Purified RNA used for the gene...
Catalog: 4378672
Size: 24 preps
Size: 24 preps
The ARCTURUS® Paradise® PLUS FFPE RNA Isolation Kit permits unprecedented gene expression analysis of millions of previously inaccessible samples by unlocking RNA from formalin-fixed, paraffin-embedded (FFPE) tissues. Amplify as little as 5 ng of the isolated fixed RNA using reagents and methods that have been specially optimized to overcome the challenges of cross-linked FFPE templates (Figure 1). Secure Downstream Success with the Paradise® QC Kit Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and th...
Catalog: KIT0312-I
Size: 12 samples
Size: 12 samples
The SuperScript® Double-Stranded cDNA Synthesis Kit contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality double-stranded cDNA from total RNA or poly A + selected RNA (mRNA). The advanced capabilities of SuperScript® II Reverse Transcriptase, with its reduced RNase H activity, maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis. Features of the SuperScript® Double-Stranded cDNA Synthesis Kit include: • Reliability —Each buffer, reagent, and enzyme in the kit is of the same high quality you expect from Life Technologie...
Catalog: 11917-010
Size: 10 reactions
Size: 10 reactions
The Ambion® TotalPrep™ RNA Amplification Kit (patent pending) is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix® arrays. The kit includes sufficient reagents for 96 reactions. • Perform amplification from just 50 ng of input RNA • Developed for expression profiling using the Sentrix® BeadChips • Bio-16-UTP now included in optimized NTP mix, reducing setup time and maximizing labeling The TotalPrep™ RNA Amplification Kit incorporates extensive improvements to the in vitro transcription reaction (IVT) over the previous TotalPrep™ Kit. The IVT...
Catalog: 4393543
Size: 96 reactions
Size: 96 reactions
The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. • Rapid, Non-Hazardous RNA purification capabilities • Ready-to-use RNA for many downstream applications Rapid and Convenient Column Purification Procedures The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and t...
Catalog: 12183018A
Size: 50 preps
Size: 50 preps
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from PAXgene® Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis. • Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit • Simple magnetic bead workflow—no spin columns • Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors Maximize Total ...
Catalog: 4451894
Size: 96 preps
Size: 96 preps
Use the iPrep™ Trizol® Plus RNA Kit for the highest purity, even from difficult samples such as fatty tissue. This kit provides reproducible RNA sample preparation from up to 50 mg of tissue, 10e7 cultured cells, 10 ml blood, and plant samples. The kit contains pre-filled, fully qualified cartridges to avoid any mix-ups or cross-contamination. It also provides low CVs and reduced purification variability, increasing the reliability of your gene expression assays.
Catalog: IS-10007
Size: 1 kit
Size: 1 kit
T7 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage T7 promoter sequences. The enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available. Application: Synthesis of labeled and unlabeled RNA transcripts (1). Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid. Performance and Quality Testing: 3´ and 5´ exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction. Unit De...
Catalog: 18033-100
Size: 2 × 2,500 units
Size: 2 × 2,500 units
The SuperScript® III First-Strand Synthesis System for RT-PCR provides all the necessary components for synthesis of high-quality first-strand cDNA from total or poly(A)+ RNA. Used with PCR, this functionally tested system enables you to detect rare messages and generate enough material for cloning. The SuperScript® III First-Strand Synthesis System: Uses SuperScript® III RT for higher cDNA yields (Figure 1) Includes all components needed for first-strand cDNA synthesis Consists of optimized reagents for more robust performance Contains a streamlined protocol Detects template molecules from as...
Catalog: 18080-051
Size: 50 reactions
Size: 50 reactions
0.5-10 Kb RNA Ladder is suitable for sizing single-stranded RNA ranging from 0.5 kb to 10 kb in glyoxal or formaldehyde agarose gels. The ladder consists of nine bands in even increments (0.5, 1, 1.5, 2, 3, 4, 6, 8, and 10 kb) to simplify size estimation. The 1.5 kb band is more intense than the other bands, allowing easy orientation and identification of RNA bands on the gel. All RNA molecules in the ladder are polyA-tailed (40 nt) for convenient use as control templates for cDNA synthesis.
Catalog: 15623-200
Size: 75 µg
Size: 75 µg
The highest-quality RNA available from Ambion®, it is DNase-treated and subjected to unsurpassed quality control standards. One tube containing 100 µg is provided at a concentration of 1 mg/mL. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects used to judge the quality of FirstChoice® RNAs. The samples are processed using Ambion® RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment is performed to ensure that the RNA is ready for use in any ...
Catalog: AM7846
Size: 100 µg
Size: 100 µg
THE Ambion® RNA Storage Solution is a buffer that provides greater RNA stability than standard 0.1 mM EDTA or TE Buffer. This 1 mM sodium citrate, pH 6.4 +/- 0.2 buffer is provided in one bottle containing 50 mL. The last step in every RNA isolation protocol is to resuspend the purified RNA pellet. After painstakingly isolating the RNA, it is crucial that the pellet be suspended and stored in a safe, RNase-free solution. THE RNA Storage Solution has two features that minimize base hydrolysis of RNA: a low pH, and sodium citrate, which is an efficient chelating agent. THE RNA Storage Solution i...
Catalog: AM7001
Size: 50 ml
Size: 50 ml
This Ambion® kit is for the rapid, high-throughput purification of RNA from mammalian cells and tissue in 96-well plates. The kit combines an organic extraction with magnetic bead–based purification yielding extremely high-quality RNA suitable for the most stringent applications, such as microarray analysis. The kit includes sufficient reagents for 96 reactions. • Improve gene expression studies with consistent results from experiment to experiment • Combines TRI Reagent® with Ambion's superior magnetic bead-based technology • Streamlined RNA purification, without sacrificing RNA quality or qu...
Catalog: AM1839
Size: 96 purifications
Size: 96 purifications
The SuperScript® III First-Strand Synthesis SuperMix provides high yields of first-strand cDNA in a convenient high-throughput supermix format. The simple, time-saving reaction set-up uses just two tubes-a 2X Reaction Mix and a SuperScript® III Enzyme Mix (Figure 1). An optional annealing buffer is provided for improved yields and sensitivity. The Enzyme Mix includes SuperScript® III Reverse Transcriptase to ensure higher cDNA yields, superior sensitivity-down to 0.1 pg (Figure 2)-and specificity, and compatibility with a wide variety of RT-PCR applications.
Catalog: 18080-400
Size: 50 reactions
Size: 50 reactions
The highest-quality RNA available from Ambion®, it is DNase-treated, subjected to unsurpassed quality control standards, and certified to contain small RNAs (miRNA, siRNA, and snRNA). One tube containing 100 µg is provided at a concentration of 1 mg/mL. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects used to judge the quality of FirstChoice® RNAs. The samples are processed using Ambion® RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment ...
Catalog: AM7960
Size: 100 µg
Size: 100 µg
The PureLink® Viral RNA/DNA Mini Kit is a nucleic acid purification system designed for fast and easy isolation of viral RNA or DNA from cell-free samples, such as serum, plasma, and cerebrospinal fluid. Starting sample volumes can be as high as 500 µl and elution volumes as low as 10 µl, allowing for a 50-fold concentration of nucleic acids. The higher target concentration obtained using the PureLink® Viral RNA/ DNA Mini Kit provides greater sensitivity and accuracy in downstream detection procedures such as real-time PCR or endpoint analysis. Specially formulated buffers enable the purificat...
Catalog: 12280-050
Size: 50 preps
Size: 50 preps
The NCode™ VILO™ miRNA cDNA Synthesis Kit is part of a novel quantitative assay that provides flexibility, sensitivity, and specificity when quantitating miRNA expression. The system combines a carefully optimized polyadenylation reaction with the market-leading reverse transcriptase, SuperScript® III RT, in a universal first-strand cDNA synthesis reaction. Tried and tested SuperScript® III Reverse Transcriptase is formulated in an enhanced buffer system to provide you with the most reliable firststrand synthesis and higher cDNA yields, with sensitivity and accurate quantitation from 10pg to 1...
Catalog: A11193-050
Size: 50 reactions
Size: 50 reactions
RNAlater®-ICE is a novel reagent for transitioning frozen tissue to a state that is easily processed for extraction of high quality RNA. Frozen tissues are simply submerged in RNAlater®-ICE and allowed to thaw overnight at -20°C. Once thawed, the tissues can be processed like fresh tissues using standard RNA isolation procedures. No more laborious grinding of frozen tissue to safeguard the RNA. • Process previously frozen tissues like freshly harvested samples • Thawing tissues in RNAlater®-ICE protects RNA from degradation • No more tissue pulverizing with mortar and pestle and awkward transf...
Catalog: 4427575
Size: 10 bottles
Size: 10 bottles
Rna Synthesis
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