rna amplification

Illumina® TotalPrep™ RNA Amplification Kit

Category: Products & Services > Kits & Assays > RNA Amplification Kits

The Ambion® Illumina® TotalPrep™ RNA Amplification Kit (patent pending) is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix® arrays. The new TotalPrep™ RNA Amplification Kit incorporates extensive improvements to the in vitro transcription reaction (IVT) over the previous Illumina® RNA Amplification Kit. Experience these Improvements: • Improved first-strand cDNA synthesis using ArrayScript™ reverse transcriptase • Biotinylated UTP allows ease of use and optimal labeling • Maximization of cRNA yield using MEGAscript® IVT technology • Signific...
Catalog: AMIL1791
Size: 24 reactions

Illumina® TotalPrep™-96 RNA Amplification Kit

Category: Products & Services > Kits & Assays > RNA Amplification Kits

The Ambion® TotalPrep™ RNA Amplification Kit (patent pending) is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix® arrays. The kit includes sufficient reagents for 96 reactions. • Perform amplification from just 50 ng of input RNA • Developed for expression profiling using the Sentrix® BeadChips • Bio-16-UTP now included in optimized NTP mix, reducing setup time and maximizing labeling The TotalPrep™ RNA Amplification Kit incorporates extensive improvements to the in vitro transcription reaction (IVT) over the previous TotalPrep™ Kit. The IVT...
Catalog: 4393543
Size: 96 reactions

DNase I, Amplification Grade

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > DNA⁄RNA Modifying Enzymes

DNase I, Amplification Grade digests single- and double-stranded DNA to oligodexyribonuleotides. The specific activity is >10,000 units/mg. DNase I, Amplification Grade is purified and tested for non-detectable levels of RNase contamination. Absence of RNase is tested by performing a Ribonuclease assay with RNA ladder. Application: Removing DNA from RNA (1) and protein preparations. Source: Purified from bovine pancreas. Performance and Quality Testing: Ribonuclease assay with RNA ladder; ability to digest single-stranded and double-stranded DNA to oligonucleotides determined. Unit Definition:...
Catalog: 18068-015
Size: 100 units

Ambion® WT Expression Kit, Manual Use

Category: Products & Services > Kits & Assays > RNA Amplification Kits

The WT Expression Kit is an RNA amplification innovation from Ambion® that is designed to generate amplified sense-strand cDNA ready for fragmentation and labeling using the Affymetrix® GeneChip® WT Terminal Labeling Kit. Features of the Ambion® WT Expression Kit: • Optimized for use with Affymetrix® GeneChip® Human, Mouse, and Rat 1.0 ST Arrays • Allows RNA samples as small as 50 ng to be analyzed on Affymetrix® GeneChip® Arrays • Supports a streamlined workflow that does not require a separate rRNA depletion step • Is ideal for high-sensitivity expression profiling Eliminates Separate rRNA D...
Catalog: 4411974
Size: 30 reactions