Reversed-Phase Columns
Applied Biosystems® MultiScribe™ Reverse Transcriptase is a recombinant Moloney murine leukemia virus (rMoMuLV) reverse transcriptase. MultiScribe™ Reverse Transcriptase is similar to MuLV reverse transcriptase but differs in its recommended usage. MultiScribe™ Reverse Transcriptase is a recombinant RNA-dependent DNA polymerase that uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand. 5000U supplied in 100 µl (50U/ µl). • Compatible with one-step and two-step RT-PCR reactions, which provides flexibility in assay set-up • Individual...
Catalog: 4311235
Size: 100 µl
Size: 100 µl
The microcentrifuge spin columns included with the FastTrack® 2.0 and Micro-FastTrack™ 2.0 Kits are available separately. The columns are made from polypropylene and are designed to enhance the speed and efficiency of polyA + RNA isolation. The columns are sterilized and RNase-free.
Catalog: R500-20
Size: 200 columns
Size: 200 columns
SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A) + RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity...
Catalog: 18080-044
Size: 10,000 units
Size: 10,000 units
SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics. • Yield – Reduced RNase H activity results in greater first-strand cDNA yields • Size of ...
Catalog: 18064-071
Size: 4 × 10,000 units
Size: 4 × 10,000 units
Applied Biosystems® MuLV Reverse Transcriptase is a recombinant RNA-dependent DNA polymerase that uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand. It can be used for the preparation of cDNA libraries, or for first strand synthesis in RT-PCR, or in dideoxy sequencing reactions in place of Klenow enzyme for G+C-rich regions. MuLV Reverse Transcriptase lacks endonuclease activity and has extremely low RNase H activity compared to AMV reverse transcriptase.
Catalog: N8080018
Size: 5000 units
Size: 5000 units
Cloned Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT) is highly purified from insect cells infected with baculovirus containing the pol gene of AMV (1). The resulting enzyme has a higher specific activity than does native AMV RT, providing enhanced cDNA yields and improved sensitivity. The enzyme, accompanying buffers, and protocols are optimized to synthesize first-strand cDNA for amplification by PCR, and for cloning double-stranded cDNA of up to 12 kb (Figure 2). AMV RT has endoribonuclease H activity. • Flexibility —Works with oligo(dT), random primers, or a gene-specific prim...
Catalog: 12328-019
Size: 750 units
Size: 750 units
Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS® CaptureSelect® FSH HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of CaptureSelect® ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH). Feature...
Catalog: 4481828
Size: 1 each
Size: 1 each
Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS® CaptureSelect® FSH HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of CaptureSelect® ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH). Feature...
Catalog: 4481822
Size: 1 each
Size: 1 each
Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS® CaptureSelect® FSH HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of CaptureSelect® ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH). Feature...
Catalog: 4481826
Size: 1 each
Size: 1 each
Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS® CaptureSelect® FSH HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of CaptureSelect® ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH). Feature...
Catalog: 4481824
Size: 1 each
Size: 1 each
GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS® ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features: • Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform • Direct connection to standard chromatography systems • Packed with POROS® high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates The resin in this GoPure™ D 50 µm Column has a dimethylaminopropyl functional group for stro...
Catalog: 4481319
Size: 1 column
Size: 1 column
GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS® ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features: • Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform • Direct connection to standard chromatography systems • Packed with POROS® high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates The resin in this GoPure™ HQ 50 µm Column has a quaternized polyethyleneimine functional gro...
Catalog: 4481315
Size: 1 column
Size: 1 column
GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS® ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features: • Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform • Direct connection to standard chromatography systems • Packed with POROS® high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates The resin in this GoPure™ PI 50 µm Column has a polyethyleneimine functional group for stron...
Catalog: 4481318
Size: 1 column
Size: 1 column
GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS® ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features: • Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform • Direct connection to standard chromatography systems • Packed with POROS® high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates The resin in this GoPure™ HS 50 µm Column has a sulphopropyl functional group for strong cat...
Catalog: 4481316
Size: 1 column
Size: 1 column
GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS® ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features: • Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform • Direct connection to standard chromatography systems • Packed with POROS® high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates The resin in this GoPure™ XS Column has a sulphopropyl functional group for strong cation ex...
Catalog: 4481317
Size: 1 column
Size: 1 column
SKP2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. In tumor tissues, SKP2 is expressed widely, such as colon mucinous carcinoma, gastric adenocarcinoma, breast cancer, melanoma, prostate adenocarcinoma, with particularly higher expression on lung adenocarcinoma, lung squamous carcinoma, squamous carcinoma of cervix, endometrial carcinoma, ovary serous carcinoma and leiomyosarcoma. A recent study suggests that SKP2 plays a role in oncogenesis and is involved in the pathogenesis of lymphomas. Positive tissue: Breast ca...
Catalog: 18-7334
Size: 0.5 ml
Size: 0.5 ml
It is a sequencing primer to sequence mammalian expression vectors having the BGH polyadenylation signal. Primer is supplied as 2 µg which equals 358 pMoles. The melting temperature is 54.8°C. The sequence of the primer is: 5'd[TAGAAGGCACAGTCGAGG]3'
Catalog: N575-02
Size: 2 µg
Size: 2 µg
The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of input total RNA in a single 20 µL reaction to single stranded cDNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Includes sufficient reagents for 1000 x 20 µL reactions. • Linear target amplification for real-time PCR • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost • 10-fold greater dynamic range than other kits cDNA from Total RNA The High Capacity cDNA Reverse Transcription Kit (for...
Catalog: 4374967
Size: 1000 reactions
Size: 1000 reactions
This Applied Biosystems® kit provides the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, and oligo (dT) 16 , reverse primers are included for cDNA synthesis flexibility. Supplied as 2000 reactions in 10 tubes (200 reactions/tube). • Contains MultiScribe® Reverse Transcriptase, a recombinant Moloney Murine Leukemia Virus Reverse Transcriptase • Random hexamers and oligo (dT) 16 included for flexibility • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions TaqMan® Reverse Transcription Reagents provide all...
Catalog: 4304134
Size: 2000 reactions
Size: 2000 reactions
The Applied Biosystems® TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for 200 reactions of optimal performance in TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA. A highly specific kit that quantitates only mature miRNAs, not precursors. A sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is fast, simple and scalable 2-step quantitative RT-PCR assay that provides high-quality results in less than three hours.
Catalog: 4366596
Size: 200 reactions
Size: 200 reactions
Reversed-Phase Columns
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