Reverse Transcriptase Enzyme Buffer

MultiScribe™ Reverse Transcriptase

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Reverse Transcriptase

Applied Biosystems® MultiScribe™ Reverse Transcriptase is a recombinant Moloney murine leukemia virus (rMoMuLV) reverse transcriptase. MultiScribe™ Reverse Transcriptase is similar to MuLV reverse transcriptase but differs in its recommended usage. MultiScribe™ Reverse Transcriptase is a recombinant RNA-dependent DNA polymerase that uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand. 5000U supplied in 100 µl (50U/ µl). • Compatible with one-step and two-step RT-PCR reactions, which provides flexibility in assay set-up • Individual...

Catalog: 4311235
Size: 100 µl

SuperScript® III Reverse Transcriptase

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Reverse Transcriptase

SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A) + RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity...

Catalog: 18080-044
Size: 10,000 units

SuperScript® II Reverse Transcriptase

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Reverse Transcriptase

SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics. • Yield – Reduced RNase H activity results in greater first-strand cDNA yields • Size of ...

Catalog: 18064-071
Size: 4 × 10,000 units

MuLV Reverse Transcriptase

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Reverse Transcriptase

Applied Biosystems® MuLV Reverse Transcriptase is a recombinant RNA-dependent DNA polymerase that uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand. It can be used for the preparation of cDNA libraries, or for first strand synthesis in RT-PCR, or in dideoxy sequencing reactions in place of Klenow enzyme for G+C-rich regions. MuLV Reverse Transcriptase lacks endonuclease activity and has extremely low RNase H activity compared to AMV reverse transcriptase.

Catalog: N8080018
Size: 5000 units

AMV Reverse Transcriptase, Cloned

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Reverse Transcriptase

Cloned Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT) is highly purified from insect cells infected with baculovirus containing the pol gene of AMV (1). The resulting enzyme has a higher specific activity than does native AMV RT, providing enhanced cDNA yields and improved sensitivity. The enzyme, accompanying buffers, and protocols are optimized to synthesize first-strand cDNA for amplification by PCR, and for cloning double-stranded cDNA of up to 12 kb (Figure 2). AMV RT has endoribonuclease H activity. • Flexibility —Works with oligo(dT), random primers, or a gene-specific prim...

Catalog: 12328-019
Size: 750 units