Restriction Enzyme Buffers

GeneAmp® 10X PCR Gold Buffer & MgCl2, 6 Sets (6 x 1.5 mL)

Category: Products & Services > Buffers & Chemicals > Molecular Biology Enzyme Buffers > PCR Enzyme Buffers

Applied Biosystems® GeneAmp®10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold® DNA Polymerase, resulting in highly specific, robust PCR amplification. When diluted 1:10, provides the optimal pH and ionic strength for PCR activation and amplification with AmpliTaq Gold® DNA Polymerase, ensuring maximum performance Available in several convenient sizes GeneAmp® 10X PCR Buffer II is both magnesium ion free and gelatin free. A separate MgCl 2 solution is provided. When used together, GeneAmp® 10X PCR Buffer II and MgCl 2 solution offer more versatility in...
Catalog: 4306898
Size: 6 × 1.5 ml

Taq DNA Polymerase PCR Buffer, includes 1ml of 50 mM MgCl 2

Category: Products & Services > Buffers & Chemicals > Molecular Biology Enzyme Buffers > PCR Enzyme Buffers

Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 ml of 50 mM MgCl 2 . It is included with Platinum® Taq, Taq, and the SuperScript® First-Strand Synthesis System for RT-PCR.
Catalog: 18067-017
Size: 2 × 1 ml

Cell Dissociation Buffer, enzyme-free, PBS

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Cell Dissociation Buffers

Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS). Gibco® Cell Dissociation Buffer is suitable for the gentle dissociation of mammalian cells for studies such as ligand binding, flow cytometry, and immunohistochemistry, that require intact cell surface proteins. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines, such as HeLa and NIH 3T3, but is not recommended for the routine passaging of strongly adherent...
Catalog: 13151-014
Size: 100 ml

AmpliTaq® DNA Polymerase with Buffer I

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits

AmpliTaq® DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus ( Taq ) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot. Features of this enzyme: AmpliTaq® DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy Its thermal activity profile makes it ...
Catalog: N8080160
Size: 250 units

Sma I

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Restriction Enzymes

5´-CCC↓GGG-3´ 3´-GGG↑CCC-5´ Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns. Concentration 4-12 U/ µL Contents Sma I 10X Buffer T (1mL) 10X Loading Buffer (1mL) Reaction Temperature 30°C Reaction Mixture Sma I 1 µL 10X Buffer T 2 µL 0.10% BSA 2 µL Substrate DNA Heat inactivation Enzyme inactivated by heating at 65°C for 20 minutes....
Catalog: 15228-018
Size: 1,000 units

Xba I

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Restriction Enzymes

5´-T↓CTAGA-3´ 3´-AGATC↑T-5´ Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns. Concentration 8-20 U/ µL Contents Xba I 10X Buffer M (1mL) 10X Loading Buffer (1mL) Reaction Temperature 37°C Reaction Mixture Xba I 1 µL 10X Buffer M 2 µL 0.10% BSA 2 µL Substrate DNA Heat inactivation Enzyme inactivated by heating at 65°C for 20 minutes....
Catalog: 15226-012
Size: 2,000 units