Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 8.6 cm x 13.5 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to ...
Invitrogen makes blotting easier by providing a variety of pre-cut, pre-assembled membrane/filter-paper sandwiches. A protein's properties ( i.e. , charge, hydrophobicity, etc.) affect its ability to bind to membrane surfaces. Finding the right membrane may require experimenting with your specific protein on different membranes. Store all membranes at room temperature.