Protein Production

PKN1 (PRK1) Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases

PKN1 (PRK1) belongs to the protein kinase C superfamily. This kinase is activated by the Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can also be activated by phospholipids.
Catalog: PV3790
Size: 10 µg

Maltose Binding Protein Mouse Monoclonal Antibody (clone R29)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

Mouse anti-Maltose Binding Protein; Unconjugated Monoclonal antibody specific to Multiple Maltose Binding Protein. This antibody is validated for use in Western Blot, Immunoassay (ELISA). Anti-Maltose Binding Protein recognizes the expressed product of the MBP gene.
Catalog: 33-5100
Size: 100 µg

SKP2 (S-Phase Kinase-Associated Protein 2) Mouse Monoclonal Antibody (clone 2C8D9), Concentrate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

SKP2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. In tumor tissues, SKP2 is expressed widely, such as colon mucinous carcinoma, gastric adenocarcinoma, breast cancer, melanoma, prostate adenocarcinoma, with particularly higher expression on lung adenocarcinoma, lung squamous carcinoma, squamous carcinoma of cervix, endometrial carcinoma, ovary serous carcinoma and leiomyosarcoma. A recent study suggests that SKP2 plays a role in oncogenesis and is involved in the pathogenesis of lymphomas. Positive tissue: Breast ca...
Catalog: 18-7334
Size: 0.5 ml

MAP3K14 (NIK) Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases

MAP3K14 (NIK) is structurally related to MAP3Ks. MAP3K14 phosphorylates and activates CHUK (IKKalpha) and IKBKB (IKK beta), which in turn phosphorylate IkB-alpha, releasing NF-KappaB leading to up-regulation of inflammatory response genes.
Catalog: PV4902
Size: 10 µg

I-Block™ Protein-Based Blocking Reagent

Category: Products & Services > Buffers & Chemicals > Immunoassay Buffers & Reagents > ELISA Buffers & Reagents

This Casein-based blocking reagent can be used with Western-Light™, Western-Light Plus™, Western-Star™, ELISA-Light™, Southern-Light™, and Southern-Star™ Detection Systems. For Research Use Only. Not for use in diagnostics procedures.
Catalog: T2015
Size: 30 g

PFHM-II Protein-Free Hybridoma Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Hybridoma Media

Gibco® Protein Free Hybridoma Medium II (PFHM II) is a serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® PFHM II features: • Ability to support suspension and stationary hybridomas and myelomas • Complete, ready-to-use formulation • Serum-free, protein-free formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-depende...
Catalog: 23600042
Size: 1 x 10L

CaptAvidin™ Biotin-Binding Protein

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Avidin

CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a K a of ~10 9 M -1 . This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex.
Catalog: C-21385
Size: 1 mg

Recombinant Protein G - Sepharose® 4B

Category: Products & Services > Columns, Resins & Plates > Chromatographic Columns & Media > Affinity Media & Standards

Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This product is prepared by covalently coupling highly purified rec-Protein G to Sepharose® 4B. It is supplied as a 50% suspension containing 25 ml rec-Protein G-Sepharose® 4B + 25 ml PBS (10 mM phosphate-buffered saline, pH 7.4, containing 0.1% Proclin preservative). Applications: Immunoglobulin affinity purification and Immunoprecipitation. Recombinant protein G binds to the Fc region of many types of immunoglobulin G including: all four subclasses of human IgG mouse IgG rabb...
Catalog: 10-1243
Size: 25 ml

Avidin, NeutrAvidin® Biotin-binding Protein

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Neutravidin

Our NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding.
Catalog: A-2666
Size: 5 mg

Protein Quant Sample Lysis Kit, 25 mL

Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents

The Protein Quant Sample Lysis Kit contains the reagents required for the preparation of target and control cell lysates for use with TaqMan® Protein Assay experiments. It contains a one-step lysis solution for preparing crude cell lysates from cultured cells, and the lysates are compatible with cell counting as well as total protein quant measurements such as a microBCA assay. The Protein Quant Sample Lysis Kit contains a buffered nonionic detergent solution for the gentle lysis of adherent and non-adherent cultured cells under non-denaturing conditions
Catalog: 4448536
Size: 25 ml

Protein Thermal Shift™ Software v1.0--Additional License

Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software

The Protein Thermal Shift™ Software v1.0 was developed to analyze protein melt fluorescent readings directly from Applied Biosystems® real-time PCR instrument files. Different proteins will have different Protein Thermal Shift™ profiles, each with a unique protein melt curve shape, slope, signal-to-noise ratio, and temperature melt range (see figure at right for an example). The Protein Thermal Shift™ Software generates one or multiple melting temperature values (Tm) from these curves by two methods: the Boltzmann-derived Tm and the Derivative Curve-determined Tm, to serve as points of compari...
Catalog: 4466038
Size: 1 license

BMPR1A (ALK3) Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases

BMPR1A is a Type I member of the TGF beta receptor superfamily of transmembrane ser⁄thr kinases which phosphorylates intracellular SMADs in response to bone morphogenic proteins.
Catalog: PV6038
Size: 10 µg

Protein A-HRP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Detection Kits & Reagents > Protein A

Protein A from Staphylococcus aureus, Cowan I Strain conjugated with highly purified horseradish peroxidase (RZ > 3.0). The product is liquid containing 10 mM phosphate, 0.15 M NaCl buffer, pH 7.4, 1% ovalbumin, 40% glycerol, and 0.1% proclin as preservative.
Catalog: 10-1023
Size: 3 mg

PichiaPink™ Secreted Protein Kit

Category: Products & Services > Kits & Assays > Protein Expression Kits > Yeast Expression Kits

The PichiaPink™ Secreted Protein Expression Kit is a new recombinant protein expression system based on the yeast Pichia pastoris . The PichiaPink™ Secreted Protein Expression Kit gives you convenient and cost-efficient bioproduction from shake flask to 1000-liter production of your recombinant protein. The PichiaPink™ Secreted Protein Expression Kit comes with our new Pichia strains, the pPINK-HC and pPINK-LC vectors, and secretion signal sequences for production of your protein. • Get started with secreted protein production in Pichia pastoris . • Screen your Pichia transformants rapidly by ...
Catalog: A11151
Size: 1 kit

MAPK9 (JNK2) Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases

MAPK9 (JNK2) is a serine⁄threonine kinase which plays an important role in the MAPK pathway.
Catalog: PV4018
Size: 1 mg

FCGR3A (CD16a) Recombinant Human Protein, His Tag, Active

Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers

Fc gamma receptor III-a (FCGR3A/CD16a), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1- Gln 208) of human FCGR3A (P08637-1) fused to a polyhistidine tag at the C-terminus. Activity: Using the Octet RED System, the affinity constant (Kd) of CD16a bound to Human IgG1 was 180nM. Formulat...
Catalog: 10389-H08H-250
Size: 5 × 50 µg

CD27 Recombinant Human Protein, hIgG1-Fc.His Tag, Active

Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers

Cluster of Differentiation 27 (CD27), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ile 192) of human CD27 (NP_001233.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the carboxy-terminus. Activity: Measured by its ability to bind to recombinant human CD70 . Immobilized recombinant human CD27 at 2 µg/ml (100 µl/well) c...
Catalog: 10039-H03H-25
Size: 25 µg

CD27 Recombinant Human Protein, hIgG1-Fc.His Tag, Active

Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers

Cluster of Differentiation 27 (CD27), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ile 192) of human CD27 (NP_001233.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the carboxy-terminus. Activity: Measured by its ability to bind to recombinant human CD70 . Immobilized recombinant human CD27 at 2 µg/ml (100 µl/well) c...
Catalog: 10039-H03H-5
Size: 5 µg

CD36 Recombinant Human Protein, His Tag, Active

Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers

Cluster of Differentiation 36 (CD36), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Gly 30-Asn 439) of human CD36 (NP_001001547.1) fused both to a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus. Activity: Measured by its binding ability in a functional ELISA . I...
Catalog: 10752-H08H-25
Size: 25 µg

FCGR1A (CD64) Recombinant Human Protein, His Tag, Active

Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers

Fc gamma receptor I-a (FCGR1A/CD64), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Pro 288) of human FCGR1A (NP_000557.1) fused to a polyhistidine tag at the C-terminus. Activity: Measured by its binding ability in a functional ELISA 1. Immobilized CD64 at 10 µg/ml can bind human IgG...
Catalog: 10256-H08H-25
Size: 25 µg