Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Protein Labeling Kits
The FluoReporter® FITC Protein Labeling Kit provides a convenient and flexible means for attaching fluorescein (FITC, Ex/Em ~494/518 nm) to your monoclonal or polyclonal antibody (or any protein >30 kDa). The kit supplies the reagents needed for 5 to 10 labeling and separation reactions. Important Features of the FluoReporter® FITC Protein Labeling Kit: • Labeled proteins typically ready to use in under 3 hours with very little hands-on time • Optimized for 0.2–2.0 mg of protein with molecular weight >30 kDa • Labeled proteins are purified using convenient spin filters • Requires the antibody ...
Catalog: F-6434
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Protein Labeling Kits
Molecular Probes® Protein Labeling Kits provide a convenient means for attaching a fluorescent label (or biotin) to an antibody (or a protein larger than 40 kDa). Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, FISH, and more. Kits are available in 12 Alexa Fluor® dye colors, biotin, the hapten Oregon Green® 488, fluorescein EX, and Texas Red® dye. Each kit provides the components needed to perform three protein conjugations and purifications. Important Features of Protein La...
Catalog: A-10235
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Antibody Labeling Kits (Conjugation-Based)
Create a perfectly labeled antibody with the SiteClick™ Qdot® 565 Antibody Labeling Kit. This kit replaces the conventional Qdot® 565 Antibody Conjugation Kit (Q22031MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10450
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Antibody Labeling Kits (Conjugation-Based)
Create a perfectly labeled antibody with the SiteClick™ Qdot® 605 Antibody Labeling Kit. This kit replaces the conventional Qdot® 605 Antibody Conjugation Kit (Q22001MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10469
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Antibody Labeling Kits (Conjugation-Based)
Create a perfectly labeled antibody with the SiteClick™ Qdot® 625 Antibody Labeling Kit. This kit replaces the conventional Qdot® 625 Antibody Conjugation Kit (A10197). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody, ensuring that the antigen-binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction st...
Catalog: S10452
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Antibody Labeling Kits (Conjugation-Based)
Create a perfectly labeled antibody with the SiteClick™ Qdot® 705 Antibody Labeling Kit. This kit replaces the conventional Qdot® 705 Antibody Conjugation Kit (Q22061MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody, ensuring that the antigen-binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction ...
Catalog: S10454
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Antibody Labeling Kits (Zenon® Secondary Detection-Based)
Zenon® labeling technology provides a fast, versatile, and reliable method for adding a fluorescent label to an antibody. You need only a small amount of starting material,and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Antibody conjugates formed using Zenon® technology may be used in protocols where a directly labeled primary antibody is suitable, including flow cytometry, imaging, and high-throughput applications. This exclusive Molecular Probes® Zenon® labeling technology greatly simplifies the use of multiple mouse-derived...
Catalog: Z11203
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Antibody Labeling Kits (Conjugation-Based)
Create a perfectly labeled antibody with the SiteClick™ Qdot® 525 Antibody Labeling Kit. This kit replaces the conventional Qdot® 525 Antibody Conjugation Kit (Q22041MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10449
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Antibody Labeling Kits (Conjugation-Based)
Create a perfectly labeled antibody with the SiteClick™ Qdot® 585 Antibody Labeling Kit. This kit replaces the conventional Qdot® 585 Antibody Conjugation Kit (Q22011MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10451
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Antibody Labeling Kits (Conjugation-Based)
Create a perfectly labeled antibody with the SiteClick™ R-PE Antibody Labeling Kit. Unlike conventional antibody labeling kits, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. Depending upon the label, the resulting SiteClick™ labeled antibody can be used in flow cytometry or any other application. Importan...
Catalog: S10467
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Antibody Labeling Kits (Conjugation-Based)
Create a perfectly labeled antibody with the SiteClick™ Qdot® 655 Antibody Labeling Kit. This kit replaces the conventional Qdot® 655 Antibody Conjugation Kit (Q22021MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody, ensuring that the antigen-binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction ...
Catalog: S10453
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Antibody Labeling Kits (Conjugation-Based)
Create a perfectly labeled antibody with the SiteClick™ Qdot® 800 Antibody Labeling Kit. This kit replaces the conventional Qdot® 800 Antibody Conjugation Kit (Q22071MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody, ensuring that the antigen-binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction ...
Catalog: S10455
Size: 1 kit
Category: Products & Services > Kits & Assays > Nucleic Acid Labeling Kits
The Alexa Fluor 488 Oligonucleotide Amine Labeling Kit provides a simple method for labeling amine-modified oligonucleotides with our bright, photostable, green-fluorescent Alexa Fluor 488 dye (which is spectrally similar to fluorescein). Oligonucleotides labeled with this kit can be used as primers and as probes in hybridization experiments but should not be used for automated DNA sequencing since the Alexa Fluor dye in the kit is supplied as mixed isomers. The kits have been optimized for labeling reactions with 50 µg of a 5'-amine—modified oligonucleotide, 18 to 24 bases in length.
Catalog: A-20191
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Protein Labeling Kits
The Click-iT® HPG Alexa Fluor® 488 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 488 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 488 azide leads to a chemoselective ligation or "click" reaction between the...
Catalog: C10428
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Protein Labeling Kits
The Click-iT® HPG Alexa Fluor® 594 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 594 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 594 azide leads to a chemoselective ligation or “click" reaction between the...
Catalog: C10429
Size: 1 kit
Category: Products & Services > Kits & Assays > Protein Expression Kits > Bacterial Expression Kits
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K101-01
Size: 20 reactions
Category: Products & Services > Kits & Assays > Protein Expression Kits > Bacterial Expression Kits
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K100-01
Size: 20 reactions
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid & Protein Isolation Kits
The Ambion® PARIS™ system is for the isolation of both RNA and native protein from the same experimental sample. The kit also permits separation of nuclear and cytoplasmic fractions prior to RNA and/or protein isolation. The kit contains sufficient reagents for 50 purifications from1 to 75 mg of tissue or 100 to 10 7 cells each. • Fast, simple procedure • Isolate nuclear and cytoplasmic RNA and protein from cultured cells • Isolate total RNA and protein from cultured cells or tissues • No phenol extraction or alcohol precipitation • Reduces time, cost, and variability between independent exper...
Catalog: AM1921
Size: 50 purifications
Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Protein Analysis Kits
Applied Biosystems® Protein Thermal Shift™ kits allow users to employ a dye that binds to exposed hydrophobic residues to monitor the thermal stability of proteins using a real-time PCR instrument in order to identify buffer conditions that stabilize the protein of interest or screen libraries of ligands for compounds that bind the protein of interest. Save Time and Money with Your Sample Screening This solution significantly reduces the time and cost associated with screening samples relative to currently available methods. Protein Thermal Shift™ products that are run on our real-time PCR sys...
Catalog: 4462263
Size: 2000 reactions
Category: Products & Services > Kits & Assays > Protein Analysis Kits > Protein Quantitation Assay Kits
The CaptureSelect™ AVB Sepharose HP Leakage ELISA Kit detects AVB Sepharose HP affinity ligand contamination of adeno-associated viruses (AAV) purified using AVB Sepharose HP affinity resin (GE Healthcare). Even when covalently attached, small amounts of the AVB Sepharose HP affinity ligand can leach off of the chromatography support and co-elute with the virus particles. This ELISA provides sensitivity to detect AVB Sepharose HP affinity ligand contamination of less than 0.5 ng/mL. This assay is designed to minimize interference and to provide accurate quantitation in the presence of adeno-as...
Catalog: 810280810
Size: 10 assays