Protein Gel Electrophoresis Reagents & Buffers
UltraPure™ Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Agarose is ideal for resolving DNA and RNA fragments from 500 bp to >20 kb. Features of UltraPure™ Agarose: • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handling and less breakage • Can be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay) Improved packaging The new environ...
Catalog: 16500100
Size: 100 g
Size: 100 g
UltraPure™ Low Melting Point Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Low Melting Point Agarose is ideal for resolving DNA and RNA fragments larger than 1,000 bp and for the recovery of nucleic acid fragments after electrophoresis, as it melts at 65.5°C (below the melting point of most nucleic acids). The solution remains fluid at 37°C and will set rapidly at temperatures below 25°C. • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handlin...
Catalog: 16520100
Size: 100 g
Size: 100 g
DNA Grade Agarose has a high sieve capacity, providing optimal resolution of DNA during gel electrophoresis. The low sulfur content allows gels to be run faster without becoming brittle. Supplied as a powder that is reconstituted in 0.5X TBE for high-quality agarose gels.
Catalog: 75000-500
Size: 500 g
Size: 500 g
UltraPure™ Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Agarose is ideal for resolving DNA and RNA fragments from 500 bp to >20 kb. Features of UltraPure™ Agarose: • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handling and less breakage • Can be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay) Improved packaging The new environ...
Catalog: 16500500
Size: 500 g
Size: 500 g
A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue. Supplied in one 1.4 mL tube. All Ambion® Gel Loading Solutions are rigorously tested for non-specific endonuclease activity, exonuclease activity, RNase activity, and for functionality. These are the same solutions we use in-house and in our kits.
Catalog: AM8546G
Size: 1 ml
Size: 1 ml
BlueJuice™ Gel Loading Buffer is designed for easy loading and tracking of DNA samples in agarose or native polyacrylamide gels.
Catalog: 10816-015
Size: 3 × 1 ml
Size: 3 × 1 ml
NuPAGE® Novex 3-8% Tris-Acetate Midi-Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. NuPAGE® Novex Tris-Acetate Midi-Gels (8 x 13 cm) can run up to 26 samples. The NuPAGE Midi-Gels run in the XCell4 Surelock™ Midi-Cell and, with a Midi Gel Adapter, can run in a Bio-Rad Criterion Cell. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity,...
Catalog: LA0050
Size: 1 kit
Size: 1 kit
Denaturing polyacrylamide TBE-Urea Gels resolve single-stranded DNA oligos or RNA into sharp, distinct bands. Novex® TBE-Urea Gels are optimized for the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies, and Northern blot analysis. Novex® TBE-Urea Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE-Urea Gels are made with high-purity reagents, including tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEME...
Catalog: LC6876
Size: 10 ml
Size: 10 ml
NuPAGE® Transfer Buffer (20X) is a buffer used to transfer proteins from NuPAGE® Novex® gels to membranes for Western blotting. NuPAGE® Transfer Buffer can be used with the Novex® Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. NuPAGE® Antioxidant may be used with NuPAGE® Transfer Buffer to enhance transfer of reduced proteins to membranes. Related link Learn more about other Invitrogen™ products for your protein transfer needs .
Catalog: NP0006
Size: 125 ml
Size: 125 ml
The NativePAGE™ Running Buffer Kit contains the NativePAGE™ Running Buffer (20X), which is used to make the NativePAGE™ Cathode and Anode Running Buffers for use with an XCell SureLock® Mini Cell when running NativePAGE™ Gels. The kit also contains the NativePAGE™ Cathode Buffer Additive (20X) that is used with the NativePAGE™ Running Buffer to make the Cathode Running Buffer for the system.
Catalog: BN2007
Size: 1 kit
Size: 1 kit
UltraPure™ DNA Typing Grade® 50X TAE Buffer is a sterile-filtered solution of 2 M Tris-acetate and 50 mM EDTA. TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. To prepare a 1X solution, mix one volume of UltraPure™ 50X TAE Buffer with 49 volumes of distilled water. A 1X TAE Buffer solution contains 40 mM Tris-acetate and 1 mM EDTA with a pH of 8.3 at 23“C. Performance and Quality Testing: No DNase activity detected.
Catalog: 24710-030
Size: 1 L
Size: 1 L
NuPAGE® Transfer Buffer (20X) is a buffer used to transfer proteins from NuPAGE® Novex® gels to membranes for Western blotting. NuPAGE® Transfer Buffer can be used with the Novex® Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. NuPAGE® Antioxidant may be used with NuPAGE® Transfer Buffer to enhance transfer of reduced proteins to membranes. Related link Learn more about other Invitrogen™ products for your protein transfer needs .
Catalog: NP0006-1
Size: 1 L
Size: 1 L
Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex® DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex® TBE and DNA Retardation Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide,...
Catalog: LC6678
Size: 10 ml
Size: 10 ml
The TrackIt™ Loading Buffers are a 6X solution used for easy loading and tracking of DNA samples in agarose gels. These loading buffers contain two tracking dyes, one that runs behind the sample and one that runs ahead of the sample. The tracking dyes serve as visual markers for following migration during electrophoresis and indicate when maximum resolution of the DNA fragments has been achieved. Additionally, visualization of DNA bands will not be obscured by the tracking dyes because they run outside the limits of most DNA samples. TrackIt™ Cyan/Orange Loading Buffer is recommended for DNA f...
Catalog: 10482-028
Size: 3 × 0.5 ml
Size: 3 × 0.5 ml
Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recomme...
Catalog: LC2676
Size: 20 ml
Size: 20 ml
NuPAGE® MOPS SDS Running Buffer (20X) is formulated for running proteins on NuPAGE® Novex® Bis-Tris gels only. NuPAGE® MOPS SDS Running Buffer is recommended for separating small- to medium-sized proteins. Use the right buffer to optimize protein separations NuPAGE® MES SDS Running Buffer and NuPAGE® MOPS SDS Running Buffer can both be used with NuPAGE® Novex® Bis-Tris gels. Use of MOPS buffer allows proteins to run slower than when using MES buffer. Get consistent, convenient, high-quality results Pre-mixed NuPAGE® buffers are a convenient way to ensure high-quality, consistent electrophoresi...
Catalog: NP0001-02
Size: 5 L
Size: 5 L
Novex® Tris-Glycine Transfer Buffer (25X) is specially optimized for western blot transfer applications using Tris-Glycine gels.
Catalog: LC3675
Size: 500 ml
Size: 500 ml
NuPAGE® MES SDS Running Buffer (20X) is formulated for running proteins on NuPAGE® Novex® Bis-Tris gels only. NuPAGE® MES SDS Running Buffer is recommended for separating small- to medium-sized proteins. Use the right buffer to optimize protein separations NuPAGE® MES SDS Running Buffer and NuPAGE® MOPS SDS Running Buffer can both be used with NuPAGE® Novex® Bis-Tris gels. Use of MES buffer allows proteins to run faster than when using MOPS buffer. Get consistent, convenient, high-quality results Pre-mixed NuPAGE® buffers are a convenient way to ensure high-quality, consistent electrophoresis ...
Catalog: NP0002
Size: 500 ml
Size: 500 ml
NuPAGE® Tris-Acetate SDS Running Buffer (20X) is formulated for separation of proteins, in their denatured state, on NuPAGE® Novex® Tris-Acetate gels. NuPAGE® Novex® Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex® Tris-Acetate gels can also be run with Novex® Tris-Glycine Native Running Buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Formulation NuPAGE® Novex® Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris ba...
Catalog: LA0041
Size: 500 ml
Size: 500 ml
Novex® Tris-Glycine SDS Running Buffer (10X) is formulated for separation of proteins, in their denatured state, on Novex® Tris-Glycine gels. Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used. Separate native or denatured proteins Novex®Tris-Glycine gels do not contain SDS and can be used to accurately separate both native and denatured proteins, depending upon the sample and running buffers used. To separate denatured proteins on Novex® Tris-Glycine gels, use Novex® Tris-Glycine Native Sample Buffer a...
Catalog: LC2675-5
Size: 5 L
Size: 5 L
Protein Gel Electrophoresis Reagents & Buffers
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