Protein a Sepharose

Recombinant Protein G - Sepharose® 4B

Category: Products & Services > Columns, Resins & Plates > Chromatographic Columns & Media > Affinity Media & Standards

Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This product is prepared by covalently coupling highly purified rec-Protein G to Sepharose® 4B. It is supplied as a 50% suspension containing 25 ml rec-Protein G-Sepharose® 4B + 25 ml PBS (10 mM phosphate-buffered saline, pH 7.4, containing 0.1% Proclin preservative). Applications: Immunoglobulin affinity purification and Immunoprecipitation. Recombinant protein G binds to the Fc region of many types of immunoglobulin G including: all four subclasses of human IgG mouse IgG rabb...
Catalog: 10-1243
Size: 25 ml

PKN1 (PRK1) Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases

PKN1 (PRK1) belongs to the protein kinase C superfamily. This kinase is activated by the Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can also be activated by phospholipids.
Catalog: PV3790
Size: 10 µg

Maltose Binding Protein Mouse Monoclonal Antibody (clone R29)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

Mouse anti-Maltose Binding Protein; Unconjugated Monoclonal antibody specific to Multiple Maltose Binding Protein. This antibody is validated for use in Western Blot, Immunoassay (ELISA). Anti-Maltose Binding Protein recognizes the expressed product of the MBP gene.
Catalog: 33-5100
Size: 100 µg

SKP2 (S-Phase Kinase-Associated Protein 2) Mouse Monoclonal Antibody (clone 2C8D9), Concentrate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

SKP2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. In tumor tissues, SKP2 is expressed widely, such as colon mucinous carcinoma, gastric adenocarcinoma, breast cancer, melanoma, prostate adenocarcinoma, with particularly higher expression on lung adenocarcinoma, lung squamous carcinoma, squamous carcinoma of cervix, endometrial carcinoma, ovary serous carcinoma and leiomyosarcoma. A recent study suggests that SKP2 plays a role in oncogenesis and is involved in the pathogenesis of lymphomas. Positive tissue: Breast ca...
Catalog: 18-7334
Size: 0.5 ml

MAP3K14 (NIK) Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases

MAP3K14 (NIK) is structurally related to MAP3Ks. MAP3K14 phosphorylates and activates CHUK (IKKalpha) and IKBKB (IKK beta), which in turn phosphorylate IkB-alpha, releasing NF-KappaB leading to up-regulation of inflammatory response genes.
Catalog: PV4902
Size: 10 µg

Protein Thermal Shift™ Starter Kit

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Protein Analysis Kits

Applied Biosystems® Protein Thermal Shift™ kits allow users to employ a dye that binds to exposed hydrophobic residues to monitor the thermal stability of proteins using a real-time PCR instrument in order to identify buffer conditions that stabilize the protein of interest or screen libraries of ligands for compounds that bind the protein of interest. Save Time and Money with Your Sample Screening This solution significantly reduces the time and cost associated with screening samples relative to currently available methods. Protein Thermal Shift™ products that are run on our real-time PCR sys...
Catalog: 4462263
Size: 2000 reactions

HiMark™ Unstained Protein Standard

Category: Products & Services > Controls, Standards & Ladders > Protein Standards & Ladders > Unstained Protein Standards

The HiMark™ Unstained High Molecular Weight (HMW) Protein Standard is designed for analysis of high molecular weight proteins on Novex® NuPAGE® Tris-Acetate Gels . • Comprehensive – Contains markers with a wide range of high molecular weights, providing 9 protein bands in the range of 30-460 kDa • Convenient – A ready-to-use formulation eliminates the need to heat, reduce, or add sample buffer prior to use • Compatible – Intended for use with NuPAGE® Novex 3-8% and 7% Tris-Acetate Gels under denaturing conditions* *Note: HiMark™ Unstained High Molecular Weight Protein Standard can also be used...
Catalog: LC5688
Size: 250 µl

FluoReporter® FITC Protein Labeling Kit

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Protein Labeling Kits

The FluoReporter® FITC Protein Labeling Kit provides a convenient and flexible means for attaching fluorescein (FITC, Ex/Em ~494/518 nm) to your monoclonal or polyclonal antibody (or any protein >30 kDa). The kit supplies the reagents needed for 5 to 10 labeling and separation reactions. Important Features of the FluoReporter® FITC Protein Labeling Kit: • Labeled proteins typically ready to use in under 3 hours with very little hands-on time • Optimized for 0.2–2.0 mg of protein with molecular weight >30 kDa • Labeled proteins are purified using convenient spin filters • Requires the antibody ...
Catalog: F-6434
Size: 1 kit

PI4KB (PI4K beta) Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Lipid Kinases

PI4KB and PI4KA are the two human type III PI4 kinases, which share sequence similarly with PI3 kinases and phosphorylate the 4 position of phosphotidyl inositol.
Catalog: PV5277
Size: 20 µg

BMPR1A (ALK3) Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases

BMPR1A is a Type I member of the TGF beta receptor superfamily of transmembrane ser⁄thr kinases which phosphorylates intracellular SMADs in response to bone morphogenic proteins.
Catalog: PV6038
Size: 10 µg

FGF-Basic Recombinant Mouse Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines

Recombinant Mouse Fibroblast Growth Factor Basic (also known as FGF Basic, bFGF, FGF 2, FGF-β or Heparin Binding Growth Factor) is a bioactive protein intended for use in cell culture applications. FGF-basic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. • High purity —no interference from other proteins or contaminants • High biological activity —more results with less...
Catalog: PMG0031
Size: 100 µg

Protein A-HRP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Detection Kits & Reagents > Protein A

Protein A from Staphylococcus aureus, Cowan I Strain conjugated with highly purified horseradish peroxidase (RZ > 3.0). The product is liquid containing 10 mM phosphate, 0.15 M NaCl buffer, pH 7.4, 1% ovalbumin, 40% glycerol, and 0.1% proclin as preservative.
Catalog: 10-1023
Size: 3 mg

KDR (VEGFR2) Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Receptor Tyrosine Kinases

KDR (VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors.
Catalog: PV3660
Size: 10 µg

Alexa Fluor® 488 Protein Labeling Kit

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Protein Labeling Kits

Molecular Probes® Protein Labeling Kits provide a convenient means for attaching a fluorescent label (or biotin) to an antibody (or a protein larger than 40 kDa). Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, FISH, and more. Kits are available in 12 Alexa Fluor® dye colors, biotin, the hapten Oregon Green® 488, fluorescein EX, and Texas Red® dye. Each kit provides the components needed to perform three protein conjugations and purifications. Important Features of Protein La...
Catalog: A-10235
Size: 1 kit

PichiaPink™ Secreted Protein Kit

Category: Products & Services > Kits & Assays > Protein Expression Kits > Yeast Expression Kits

The PichiaPink™ Secreted Protein Expression Kit is a new recombinant protein expression system based on the yeast Pichia pastoris . The PichiaPink™ Secreted Protein Expression Kit gives you convenient and cost-efficient bioproduction from shake flask to 1000-liter production of your recombinant protein. The PichiaPink™ Secreted Protein Expression Kit comes with our new Pichia strains, the pPINK-HC and pPINK-LC vectors, and secretion signal sequences for production of your protein. • Get started with secreted protein production in Pichia pastoris . • Screen your Pichia transformants rapidly by ...
Catalog: A11151
Size: 1 kit

EIF2AK3 (PERK) Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases

EIF2AK3 (PERK) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK3, signaling is initiated by misfolded proteins in the ER.
Catalog: PV5106
Size: 10 µg

RORG Human Protein, Ligand Binding Domain, GST-Tagged

Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > Nuclear Receptors

Retinoid-related orphan receptor gamma, ligand binding domain (RORG-LBD) is a 58.60 kDa recombinant human protein (amino acids 250-518) expressed as a GST-tagged fusion protein in insect cells. RORG-LBD was expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator displacement studies.
Catalog: PV5888
Size: 50 μg

CDK8⁄Cyclin C Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases

CDK8 phosphorylates the carboxy-terminal domain of the largest subunit of RNA polymerase II. This protein is coexpressed and copurified with CyclinC.
Catalog: PV4402
Size: 10 µg

MagicMark™ XP Western Protein Standard (20-220 kDa)

Category: Products & Services > Controls, Standards & Ladders > Protein Standards & Ladders > Western Blot Protein Standards

The MagicMark™ XP Western Protein Standard is specifically designed for easy and convenient protein molecular weight estimation directly on western blots. The standard consists of 9 recombinant proteins each of which contains an IgG binding site. The IgG binding site binds the primary or secondary antibody used for detection of the target protein allowing direct visualization of the standard on the western blot. The MagicMark™ XP Standard is compatible with all western kits and substrates (chemiluminescent, chromogenic and fluorescent) and offers: • Direct visualization of molecular weight sta...
Catalog: LC5602
Size: 250 µl

Recombinant Protein G-HRP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Detection Kits & Reagents > Protein G

Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This protein G is conjugated with highly purified horseradish peroxidase (RZ > 3.0) and is in enyme conjugate stabilizer containing 0.1% proclin as preservative. Recombinant protein G binds to the Fc region of many types of immunoglobulin G including: all four subclasses of human IgG mouse IgG rabbit IgG guinea pig IgG rat IgG goat IgG sheep IgG cow IgG horse IgG Recombinant protein G does not bind to IgG from chicken or cat, or to human IgA, IgM, or serum albumin. It binds wea...
Catalog: 10-1223
Size: 1 ml