Trypan blue stain is a well-accepted method for staining dead cells. It can enter into cells with compromised membranes and is the method used with the Countess® automated cell counting platform.
Size: 2 × 1 ml
DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlue® Fixed Cell ReadyProbes™ Reagent, we have formulated a high-purity form of this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Features of NucBlue® Fixed Cell ReadyProbes™ Reagent: Ready-to-use liquid DAPI formulation No need to dilute, weigh, or pipette Convenient dropper bottle—just use two drops per ml Stable at room temperature—keep handy at your scope or...
Size: 1 kit
DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells. DiI is also available as a solid (D-282), as a paste (N-22880) or as crystals (D-3911).
Size: 1 ml
The Vybrant® CM-DiI cell-labeling solution is a dye delivery solution that can be added directly to normal culture media to uniformly label suspended or attached culture cells for use in cell-cell fusion, cellular adhesion and migration applications.
Size: 1 ml
The Vybrant® DiD cell-labeling solution is a dye delivery solution that can be added directly to normal culture media to uniformly label suspended or attached culture cells for use in cell-cell fusion, cellular adhesion and migration applications.
Size: 1 ml
ER-Tracker Blue-White DPX dye is a photostable probe that is selective for the endoplasmic reticulum (ER) in live cells. The cells can be aldehyde-fixed after staining with the dye but significant fluorescence signal will be lost. With excitation at ~374 nm, the environmentally-sensitive probe exhibits emission between 430 and 640 nm. A long-pass DAPI filter works best.
Size: 20 × 50 µl
The ER-Tracker™ Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY® TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K + channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell typ...
Size: 100 µg
Countess® Cell Counting Chamber Slides are designed for use with the Countess® Automated Cell Counter. Countess® slides are disposable. Each slide contains two cell counting chambers. This package comes with 1 box containing 50 slides and 2 x 1 mL vials of trypan blue (0.4%). Ready to Use For Your Convenience Each Countess® Cell Counting Chamber Slide comes presterilized and ready to use in an individual package. The Countess® slides are plastic and disposable. Cell counting occurs in a central location of the counting chamber. The volume counted is 0.4 µL, the same as counting four 1-mm 2 squ...
Size: 1 box
StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell su...
Size: 100 ml
StemPro® CD34+ cells are pooled Human Hematopoietic Progenitor cells (HPCs) derived from the umbilical cord blood of mixed donors. Along with 0.5×10 6 pooled donor cord blood-derived CD34+ cells, this kit includes StemPro®-34 SFM basal liquid medium and frozen StemPro®-34 Nutrient Supplement to facilitate the immediate culturing of the StemPro® CD34+ cells upon arrival or when recovering from cryopreservation storage. With the StemPro® CD34+ Cell Kit You Get: • ≥90% purity of the desired CD34+ cell population • Each vial of cells contains 0.5×10 6 CD34+ cells with full traceability • StemPro®-...
Size: 0.5 x 10
Mouse Embryonic stem cells (ESCs) were isolated from mouse (Strain C57BL⁄6) embryos at Day E3.5. Before cryopreservation, the cells were tested for viability (>70% post-thaw viability), their ability to remain undifferentiated when cultured on mouse feeder cells as assayed by marker expression (Oct4, SSEA-1, Nanog) and their ability to differentiate into embryoid bodies and express markers indicative of three germ layers (Ectoderm: (+) for β3-tubulin and nestin, Mesoderm: (+) for smooth muscle actin, Endoderm: (+) for AFP). Cells were cryopreserved at passage 20 (P20). For optimal growth perfo...
Size: 1 ml
The Violet Ratiometric Membrane Asymmetry Probe⁄Dead Cell Apoptosis Kit provides a simple and fast method for the detection of apoptosis with dead cell discrimination by flow cytometry. The Violet Ratiometric Membrane Asymmetry Probe, 4'-N,N-diethylamino-6-(N,N,N-dodecyl-methylamino-sulfopropyl)-methyl-3-hydroxyflavone (F2N12S), is a novel violet excitable dye for the detection of membrane asymmetry changes during apoptosis. The dye exhibits an excited-state intramolecular proton transfer (ESIPT) reaction resulting in a dual fluorescence with two emission bands corresponding to 530 nm and 585 ...
Size: 1 kit
NucGreen™ Dead 488 ReadyProbes™ Reagent is a cell-impermeant stain that emits exceptionally bright, green fluorescence when bound to DNA. Cells that have lost plasma membrane integrity are stained within minutes, making this an extremely useful stain to estimate live/dead cell ratios and to measure cytotoxicity in kinetic live-cell assays. It is also suitable for staining nuclei in fixed cell preparations and tissue sections. Like other ReadyProbes™ reagents, NucGreen™ Dead 488 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and dr...
Size: 1 kit
Alkaline Phosphatase Live Stain is a stem cell imaging product that allows users to differentially stain pluripotent stem cells (PSCs). The dye is a cell-permeable fluorescent substrate for alkaline phosphatase (AP) that is non-toxic to cells, diffusing out over the course of two hours. The Alkaline Phosphatase Live Stain consists of 50 µL of a 500X fluorescein-based dye in DMSO, sufficient for staining two 24-well plates, four 6 cm dishes or two 10 cm dishes. This Alkaline Phosphatase Live Stain allows you to: • Identify pluripotent stem cells with a strong signal above background • Stain PSC...
Size: 50 µl
The combination of the antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective action against gram-positive and gram-negative bacteria, respectively. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus . Streptomycin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibit...
Size: 100 ml
Gibco® Zeocin™ selective reagent is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus . Gibco® Zeocin™ selective reagent acts by binding to and cleaving DNA, leading to cell death. Gibco® Zeocin™ selective reagent is used for selection of yeast, bacterial and mammalian cell cultures. This product is supplied at 100 mg/ml in sterile water and is used at a working concentration ranging between 50 - 400 µg/ml depending on the cell type. Life Technologies offers a variety of antibiotics and antimycotics for cell culture applications. Product Use For Res...
Size: 50 ml
Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hydroscopicus . Hygromycin B acts by binding to the 80S subunit of the bacterial ribosome and inhibiting protein synthesis. Gibco® Hygromycin B is used as a bacterial selective antibiotic in the concentration range of 100 - 1000 µg / ml. This product is supplied as a 50 mg / ml solution. Resistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene ( hyg or hph ). Hygromycin B uses a different mode of action than Geneticin®, Blasticidin S, or Zeocin™, so it is excellent for use in dual-selec...
Size: 20 ml
Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg / ml for bacteria, or 200 – 500 µg / ml for most mammalian cells. This product is supplied as a 50 mg / ml solution in...
Size: 100 ml
Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg / ml for bacteria, or 200 – 500 µg / ml for most mammalian cells. This product is supplied as a powder and should be m...
Size: 5 g
Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger . Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the "pac" gene. Puromycin is widely used in cell biology as a selective agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2 – 5.0 µg/ml, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/ml. Gibco® Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer. Life Technologies offer...
Size: 20 ml