Primary Fibroblast Transfection
Lipofectamine® RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine® RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types. With Lipofectamine® RNAiMAX Transfection Reagent you will get: Superior transfection efficiency requiring lower RNAi concentrations leading to more effective gene knockdown with minimal non-specific effects Easy optimization due to minimal cytotoxicity across a 10-f...
Catalog: 13778-100
Size: 0.1 ml
Size: 0.1 ml
Lipofectin® Transfection Reagent is the reagent of choice for transfectin of endothelial cells; some of our customer also use it for transfection of DNA, RNA or oligonucleotides into their cell line of choice. Lipofectin® has also been shown to work well in combination with PLUS® reagent for the transfection of HeLa cells (1).
Catalog: 18292-011
Size: 1 ml
Size: 1 ml
A polyamine-based Ambion® siRNA transfection agent that can be used to transfect cells during subculturing-—saving a full day of valuable time—without increasing toxicity. It is supplied in one tube containing 0.4 mL. A successful siRNA experiment requires the appropriate transfection agent for your cells, and one that is optimized for siRNA delivery. The efficiency with which different transfection reagents deliver nucleic acids can vary dramatically with the type of nucleic acid being transfected (RNA vs. DNA, single-stranded vs. double-stranded) and the cells being used. Accessory Products:...
Catalog: AM4502
Size: 400 µl
Size: 400 µl
The Neon™ Transfection System 100 µl Kit is designed specifically for use with the Neon™ Transfection System device. Use this kit for transfection volumes of 100 µl, containing 5 × 10 5 – 2 × 10 6 adherent cells or 1 × 10 6 – 5 × 10 6 suspension cells. Cells that have been transfected using the 100 µl Neon™ Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol. This ready-to-use universal kit comes complete with enough buffer and tips to run up to 50 reactions (25 in duplicate) using optimized or user defined protocols. For Research...
Catalog: MPK10025
Size: 50 reactions
Size: 50 reactions
The Neon™ Transfection System 100 µl Kit is designed specifically for use with the Neon™ Transfection System device. Use this kit for transfection volumes of 10 µl, containing 5 × 10 4 – 2 × 10 5 adherent cells or 1 × 10 5 – 5 × 10 5 suspension cells. Cells that have been transfected using the 10 µl Neon™ Tips are then ready to be washed and plated into a multi-well culture dish. This ready-to-use universal kit comes complete with enough buffer and tips to run up to 192 reactions (96 in duplicate) using optimized or user defined protocols. For Research Use Only. Not for any animal or human the...
Catalog: MPK1096
Size: 192 reactions
Size: 192 reactions
PLUS™ Reagent is used in conjunction with transfection reagents, such as Lipofectamine™, to enhance transfection in adherent cell lines. Some customers find when using Plus™ Reagent that less optimization of DNA concentration is needed to achieve high transfection performance. The addition of Plus™ Reagent may also reduce the quantities of DNA and lipid required by approximately one-half compared to lipid alone. PLUS™ Reagent was specifically designed to expand the array of transfectable cell types (1). PLUS™ Reagent used with Lipofectamine™ Reagent has been demonstrated to improve the transfe...
Catalog: 11514-015
Size: 0.85 ml
Size: 0.85 ml
Lipofectamine® LTX Reagent offers a new, advanced solution for gene expression studies in hard-to-transfect cells. No other plasmid DNA-specific transfection reagent can match the efficiency, convenience, and gentleness of Lipofectamine® LTX Reagent for transfection of primary, difficult-to-transfect, and sensitive cell lines. Lipofectamine® LTX Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in experiments or cell lines. A certificate of origin may be obtained by contacting Technical Support. ...
Catalog: A12621
Size: 0.1 ml
Size: 0.1 ml
Collagen I, Rat Tail is the cell culture workhorse of basic coating and gel preparations for growing attachment cells. Gibco® Collagen I, Rat Tail is now available in pre-coated tissue culture plates to help save time at the bench. Designed to meet a number of different applications, our high quality, consistent Collagen I Coated Plates come in 3 formats – 96 well, 24 well and 6 well plates . • Ready to use, room temperature storage • Quality tested to help ensure consistency • Available in 3 different plate formats and in custom formats for a broad range of applications Easy to use, easy to s...
Catalog: A11428-02
Size: 5 plates
Size: 5 plates
GIBCO® Astrocyte Medium has been specially formulated to support the growth of primary human and rat astrocytes while retaining their phenotypes. The medium has been internally validated and optimized to provide an optimal balance between cell growth and the maintenance of cell viability, normal phenotype, and function. If more rapid proliferation is desired, addition of an appropriate amount of epidermal growth factor (EGF) is recommended, although cells may exhibit morphological and phenotypic changes. Astrocytes are a type of glial cells of the nervous system with a typical star shape. It h...
Catalog: A12613-01
Size: 500 ml
Size: 500 ml
A sterile set of solutions intended for use with EpiLife® medium or Medium 154 to culture human epidermal keratinocytes. The HKGS Kit provides, in separate vials, all the components of complete HKGS (S-001-5). A vial of gentamycin/amphotericin solution (GA) is also included. Use of GA is optional.
Catalog: S-001-K
Size: 1 kit
Size: 1 kit
A defined, sterile, concentrated (100X) solution intended for use with EpiLife® medium to culture human epidermal keratinocytes. EDGS is not intended for use with Medium 154. EDGS contains all of the growth factors and hormones necessary for the growth of human epidermal keratinocytes. Each bottle contains the correct amount of supplement for a 500 ml bottle of EpiLife® medium. EDGS contains: • purified bovine serum albumin • purified bovine transferrin • hydrocortisone • recombinant human insulin-like growth factor type-1 (rhIGF-1) • prostaglandin E2 (PGE2) • recombinant human epidermal growt...
Catalog: S-012-5
Size: 5 ml
Size: 5 ml
Primary Fibroblast Transfection
Previous: Primary Cells Fibroblasts | Next: Stable Transfection of Primary Cells
View products by:
Title |
Brand |
Price |
Popularity |
Best Sellers
View topsellers by:
Title |
Brand |
Price |
Popularity |
Favourites
Popular items by:
Popularity |
Our Choices |
All-Round Favourites |
Title |
Popularity |
Our Choices |
All-Round Favourites |
Title
Previous: Primary Cells Fibroblasts | Next: Stable Transfection of Primary Cells
