A polyamine-based Ambion® siRNA transfection agent that can be used to transfect cells during subculturing-—saving a full day of valuable time—without increasing toxicity. It is supplied in one tube containing 0.4 mL. A successful siRNA experiment requires the appropriate transfection agent for your cells, and one that is optimized for siRNA delivery. The efficiency with which different transfection reagents deliver nucleic acids can vary dramatically with the type of nucleic acid being transfected (RNA vs. DNA, single-stranded vs. double-stranded) and the cells being used. Accessory Products:...
Size: 400 µl
The Alexa Fluor 488 Oligonucleotide Amine Labeling Kit provides a simple method for labeling amine-modified oligonucleotides with our bright, photostable, green-fluorescent Alexa Fluor 488 dye (which is spectrally similar to fluorescein). Oligonucleotides labeled with this kit can be used as primers and as probes in hybridization experiments but should not be used for automated DNA sequencing since the Alexa Fluor dye in the kit is supplied as mixed isomers. The kits have been optimized for labeling reactions with 50 µg of a 5'-amine—modified oligonucleotide, 18 to 24 bases in length.
Size: 1 kit
Properly controlled IHC stains require that a non-immune immunoglobulin equivalent of the primary antibody be run on the sample tissue in the same way that the primary antibody is run. Any positive staining would indicate that the primary antibody stain may not be specific. These reagents can be used as negative isotype controls for our predilute primary antibodies.
Size: 18 ml
The amine-reactive Lissamine™ rhodamine B sulfonyl chloride can be used to create red-fluorescent bioconjugates with excitation/emission maxima ~568/583 nm.
Size: 1 g
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Size: 10 mg
Tetrafluorophenyl (TFP) esters are an improvement over the succinimidyl ester (SE or NHS-ester) chemistry typically used to attach fluorophores or haptens to the primary amines of biomolecules. Both reactive chemistries produce the same strong amide bond between the dye or hapten and the compound of interest, but TFP esters are less susceptible to spontaneous hydrolysis during conjugation reactions. Alexa Fluor TFP esters are stable for several hours at the basic pH typically used for reactions – far outlasting succinimidyl esters.
Size: 1 mg
The amine-reactive PyMPO succinimidyl ester can be used to create bioconjugates with this environment-sensitive fluorophore.
Size: 5 mg
The amine-reactive Alexa Fluor 430 carboxylic acid, succinimidyl ester and its conjugates exhibit the rare combination of absorption between 400 nm and 450 nm and fluorescence emission beyond 500 nm (excitation/emission maxima ~434/539 nm).
Size: 5 mg
This single 5-isomer preparation of our proprietary, amine-reactive dye, Oregon Green 488 carboxylic acid, succinimidyl ester and its conjugates have green fluorescence similar to that of fluorescein but are more photostable. Additionally, Oregon Green 488 dye has a lower pK a than fluorescein (pK a = 4.7 versus 6.4 for fluorescein), making this bright dye less pH sensitive in the physiological range.
Size: 5 mg
Pacific Green™ Succinimidyl Ester is a violet-excitable (411 nm) dye with an emission maximum at ~500 nm, allowing it to be used with other violet-excitable fluorophores (such as Pacific Blue™ or Pacific Orange™ dye) for multiparameter analysis using a single excitation source. As a class of compounds, succinimidyl esters offer an efficient and easy-to-use means for selectively linking a dye to accessible primary amine groups on proteins, modified nucleic acids, or other biomolecules. The covalent bonds formed are as stable as the peptide bonds used to link the amino acids within proteins. Pac...
Size: 250 µg
This purified Mouse Histidine (C-term) Tag Monoclonal Antibody recognizes a polyhistidine amino acid sequence at the carboxy-terminus of proteins. The free carboxy-terminus of the terminal histidine residue is a key element of the epitope recognition site of the anti-His (C-term) Antibody. The Anti-His(C-term) Monoclonal Antibody recognizes the sequence: -His-His-His-His-His-His-COOH . Validated applications for Histidine (C-term) Tag monoclonal antibody are western blotting and ELISA. Antibody Specifications: • Applications: Validated applications for Histidine (C-term) Tag Monoclonal Antibod...
Size: 50 µl
This purified Mouse anti-ErbB-2 Monoclonal Antibody is specific to human, rat, and mouse ErbB2 (HER2). Over-expression of c-erbB-2 is observed in 15–40% of primary human breast tumors and correlates with poor prognosis in node positive breast cancer. Over-expression of c-erbB-2 is also observed in ovarian, gastric, salivary, and non-small cell lung carcinomas. Validated applications for mouse anti-ErbB-2 monoclonal antibody are immunoprecipitation, immunohistochemistry, flow cytometry, and Western blotting. Antibody Attributes: • Applications: Validated applications for Mouse anti-ErbB-2 monoc...
Size: 100 µg
CD32, Mouse Anti-Human, (FITC); FITC conjugated Monoclonal antibody specific to Human CD32. This antibody is validated for use in Flow Cytometry. Anti-CD32 recognizes the expressed product of the FCGR2A gene.
Size: 0.5 ml
TCR ALPHA BETA, Hamster Anti-Mouse, (Alexa Fluor 488); Alexa Fluor 488 conjugated Monoclonal antibody specific to Mouse TCR α/β. This antibody is validated for use in Flow Cytometry. Anti-TCR α/β recognizes the expressed product of the TCRA/B gene.
Size: 1 ml
IL-12 (C15.6) PURE RT X MS; Unconjugated Monoclonal antibody specific to Mouse IL-12. This antibody is validated for use in Western Blot, Flow Cytometry, Immunoassay (ELISA), Immunocytochemistry/ Immunofluorescence. Anti-IL-12 recognizes the expressed product of the Il12a gene.
Size: 500 µg
This purified E-Cadherin Monoclonal Antibody is specific to human E-Cadherin. The expression of E-cadherin correlates inversely with the motile and invasive behavior of tumor cells. Mouse anti- E-Cadherin monoclonal antibody can be used in applications such as western blotting, immunohistochemistry, immunocytochemistry/immunofluorescence, and immunoprecipitation. This anti- E-Cadherin monoclonal antibody recognizes the product of the CDH1 gene. Antibody Specifications: • Applications: Validated applications for this E-Cadherin antibody are western blotting, immunohistochemistry, and immunoprec...
Size: 100 µg
CK2BETA [pS209] PAb; Unconjugated Polyclonal antibody specific to Human, (Mouse, Rat, Chicken) CK2β [pS209]. This antibody is validated for use in Western Blot. Anti-CK2β [pS209] recognizes the expressed product of the CSNK2B gene. Catalog number 441090G is same antibody as former catalog number 441090. 441090G contains 50% glycerol, which improves the stability and longevity of this antibody.
Size: 10 blots
GPX1 rabbit monoclonal antibody recognizes human glutathione peroxidase 1 (GPX1) in western blot and immunoprecipitation applications. Antibody Specifications Applications: Western blot, immunoprecipitation Species Reactivity: Human Clone ID: C8C4 Host Species and Isotype: Rabbit IgG
Size: 100 µl
This Antibody Pair Kit comes with matched antibody pairs to detect and quantify protein levels of rat IL-6. Also available is Buffer Kit for Antibody Pairs that saves time with premade, easy-to-use buffers and solutions optimized for use with Invitrogen Antibody Pairs. The Assay Buffer can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Buffer reagents needed to complete the reaction are sold separately under Catalog No. CNB0011.
Size: 10 plates
Ms anti-Nix; Unconjugated Monoclonal antibody specific to Human, Mouse, (Rat) Nix. This antibody is validated for use in Western Blot, Immunoassay (ELISA). Anti-Nix recognizes the expressed product of the BNIP3L gene, also known as Bnip3l, Bnip3l.
Size: 100 µg