Porost DNA⁄Rna Modifying Enzymes
E. coli DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of β-NAD between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate cohesive termini. Single-stranded nucleic acids are not substrates for this enzyme. Applications: Second-strand cDNA synthesis (1). T4 DNA Ligase alternative when blunt-end ligation is not required (2). Source: Purified from E. coli 594 (Su - ) bearing λ lysogen gt4lop-11 lig + S7 (3). Performance and Quality Testing: Endodeoxyribonuclease and 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested. Unit Definition: One unit is the...
Catalog: 18052-019
Size: 100 units
Size: 100 units
The TURBO DNA- free ™ Kit contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. Features of the Ambion® TURBO DNA- free ™ Kit include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can interfere with RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The best method for genomic DNA removal prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNase I that is much more efficient t...
Catalog: AM1907
Size: 50 reactions
Size: 50 reactions
DNA End Repair Mix offers an easy, one step process for creating blunt ends in sheared, sonicated, or restriction-cut DNA and PCR fragments for efficient ligation into blunt end cloning vectors. DNA End Repair Mix allows you to: Generate blunt ends in DNA and PCR fragments (A-tail) Repair DNA with 5′ or 3′ overhangs in 30 minutes at 37°C Heat inactivate DNA End Repair Mix in 10 minutes at 75°C A Simple Way to Make Blunt Ends With DNA End Repair Mix, DNA with 5′ or 3′ overhangs is converted to 5′ phosphorylated blunt end DNA in 30 minutes at 37°C. Once DNA has been repaired, the enzymes in DNA ...
Catalog: A14321
Size: 20 reactions
Size: 20 reactions
The TURBO DNA- free ™ Kit contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. Features of the Ambion® TURBO DNA- free ™ Kit include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can interfere with RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The best method for genomic DNA removal prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNase I that is much more efficient t...
Catalog: AM1907M
Size: 50 rxns w⁄ Manual
Size: 50 rxns w⁄ Manual
Optimized for researchers performing RNA structure, RNA sequencing, protein footprinting, and boundary experiments, Ambion® RNase V1, RNA-Grade cleaves base-paired nucleotides and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites. Supplied in one tube of 200 µl (0.1 U/ µL); optimization reagents also included. In addition to applications for RNA structural analysis, RNA-Grade ribonucleases can be used to map protein binding sites on RNAs by comparing cleavage patterns in the presence and absence of an RNA binding p...
Catalog: AM2275
Size: 200 µl
Size: 200 µl
S1 Nuclease is a single-strand-specific endonuclease that hydrolyzes single-stranded RNA or DNA into 5´ mononucleotides. The enzyme will hydrolyze single-stranded regions in duplex DNA such as loops and gaps. S1 Nuclease is stable at 65°C. Applications: Nuclease mapping techniques (1,2). Removal of single-stranded regions from double-stranded DNA (3). Exo III-ordered sequencing (4). Source: Isolated from Aspergillus oryza e. Performance and Quality Testing: Double-strand-specific deoxyribonuclease and phosphatase assays. Unit Definition: One unit hydrolyzes 1 µg of denatured DNA to acid-solubl...
Catalog: 18001-016
Size: 20,000 units
Size: 20,000 units
AmpErase® Uracil N-Glycosylase (UNG), part of the GeneAmp® PCR Carry-over Prevention Kit, is a 26 kDa ultrapure, recombinant enzyme encoded by the E. coli uracil N-glycosylase gene, which has been inserted into an E. coli host to direct the expression of the wild type form of the enzyme. The enzyme removes any uracil incorporated into single- or double-stranded DNA. GeneAmp® PCR Carry-over Prevention Kit The GeneAmp® PCR Carry-over Prevention Kit (available separately) provides reagents for a simple yet powerful method of ensuring that PCR products cannot be reamplified in subsequent PCR ampli...
Catalog: N8080096
Size: 100 units
Size: 100 units
Ambion® RNase Cocktail™ is a mixture of two highly purified ribonucleases, RNase A (500 U/mL) and RNase T1 (20,000 U/mL), and is free of DNase and nicking activities. Use RNase Cocktail™ for all situations where it is desirable to degrade RNA, i.e. plasmid minipreps and ribonuclease protection assays. Supplied in five tubes containing 1 mL each. Digestion of RNA with RNase A alone leaves fragments of RNA that are large enough to be visible on agarose gels and precipitate in ethanol. RNase A cuts after C and U residues, and RNase T1 cuts after G residues. Consequently, the mixture of both enzym...
Catalog: AM2288
Size: 5 tubes
Size: 5 tubes
Application/s In the isolation of DNA for RNA-free DNA. Concentration 20 mg RNase A/ml in 50 mM Tris-HCl (pH 8.0), 10 mM EDTA Specification/s An essentially protease free, freeze dried powder with an activity of not less than 70 U/mg material Source of product Bovine pancreas EC No. 3.1.27.5
Catalog: 12091-039
Size: 25 ml
Size: 25 ml
The PureLink® DNase Set provides rapid and efficient removal of DNA from RNA that has been purified using PureLink® RNA kits. The PureLink® DNase Set is: • RNase-free for use in RNA purification • Optimized for on-column digestion • Lyophilized for stability The RNase-free DNase set comes lyophilized and is optimized for on-column digestion of DNA using PureLink® protocols. The degree of DNA digestion performed by PureLink® DNase is optional for most PureLink® RNA kits, due to the efficient removal of the majority of DNA by the PureLink® technology; however, some downstream applications that a...
Catalog: 12185010
Size: 50 preps
Size: 50 preps
DNase I, Amplification Grade, digests single- and double-stranded DNA to oligodexyribonuleotides containing a 5' phosphate. DNase I, Amplification Grade, is suitable for eliminating DNA during critical RNA purification procedures such as those prior to RNA-PCR amplification. It is purified and tested for non-detectable levels of RNase contamination. Absence of RNase is tested by performing a ribonuclease assay with RNA ladder. Application Removing DNA from RNA and protein preparations. Specific activity Specific activity is >10,000 units/mg. Source Purified from bovine pancreas Performance and...
Catalog: 18068-015
Size: 100 units
Size: 100 units
T7 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage T7 promoter sequences. The enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available. Application: Synthesis of labeled and unlabeled RNA transcripts (1). Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid. Performance and Quality Testing: 3´ and 5´ exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction. Unit De...
Catalog: 18033-100
Size: 2 × 2,500 units
Size: 2 × 2,500 units
Porost DNA⁄Rna Modifying Enzymes
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