Plasmid Preps

Pippin Prep™ System (includes instrument and monitor)

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing

The Pippin Prep™ System is a small tabletop instrument that automates the size selection and collection of DNA for PGM™ sequencer libraries. Simply enter the desired base-pair target range via the software interface and after a 1.5 hr run the system will yield the programmed DNA fractions with virtually no low molecular weight contamination. The tight fragment size distributions obtained provide a more uniform template for amplification. And the processing of samples is simplified through the use of disposable pre-cast agarose gel cassettes. Benefits of the Pippin Prep™ System • Time savings c...
Catalog: 4471271
Size: 1 each

PureLink® HiPure Plasmid Maxiprep Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Plasmid DNA Purification Kits

The PureLink® HiPure Plasmid Purification Systems are designed to isolate transfection-grade plasmid DNA from E. coli in less than 2 hours. The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 ml bacterial culture, at a purity that is comparable to that achieved with two passes through cesium chloride gradients. Advantages of the PureLink® HiPure Plasmid Maxiprep Kit include: • Labor- and time-saving —No need for extra steps to remove endotoxin and other contaminants • High purity, low endotoxin product —pure enough for mammalian cell transfection • Versatility —p...
Catalog: K2100-07
Size: 25 preps

PureLink® HiPure Plasmid Megaprep Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Plasmid DNA Purification Kits

Fast, simple protocols - Purify your plasmid DNA in less than 2 hours Labor-saving - No need for extra steps to remove endotoxin and other contaminants Megapreps and Gigapreps save time by utilizing vacuum-assisted cartridges PureLink® HiPure Plasmid Purification Systems are designed to isolate plasmid DNA of the highest purity (1) with a wide scale range (Table 1). Simple protocols an anion-exchange resin allow you to purify plasmid DNA (Figure 1) at a quality equivalent to two passes through cesium chloride gradients - the most rigorous method for DNA purification. In less than 2 hours, DNA ...
Catalog: K2100-08
Size: 4 preps

Pippin Prep™ Kit 3010

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Agarose Gels

The Pippin Prep™ Kit 2010 contains 10 disposable pre-cast 3% agarose gel cassettes and a reagent pack for use isolating DNA fragments in the 50 - 300 bp range. The gel cassettes have 5 lanes, allowing 4 samples to be run along with an external size standard. The reagent pack includes that external standard as well as loading solution and electrophoresis buffer. Pippen Prep™ Kits can be purchased separately and are available in three different formats. Streamlined Pippin Prep™ System DNA Size Selection Process The workflow for Pippin Prep™ DNA size selection consists of just a few simple steps:...
Catalog: 4472172
Size: 10 reactions

Pippin Prep™ 2010 CEF Kit

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Agarose Gels

The Pippin Prep™ Kit 2010 contains 10 disposable pre-cast, ethidium-free, 2% agarose gel cassettes and a reagent pack for use isolating DNA fragments in the 100 - 600 bp range. The gel cassettes have 5 lanes, allowing 4 samples to be run along with an external size standard. The reagent pack includes that external standard as well as loading solution and electrophoresis buffer. Pippen Prep™ Kits can be purchased separately and are available in three different formats. Streamlined Pippin Prep™ System DNA Size Selection Process The workflow for Pippin Prep™ DNA size selection consists of just a ...
Catalog: 4472171
Size: 10 reactions

MagMAX™-96 DNA Multi-Sample Kit (5 x 96 Preps)

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Genomic DNA Purification Kits

MagMAX™ DNA Multi-Sample 96-Well Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells. These purification kits use MagMAX™ magnetic bead-based nucleic acid isolation technology to produce high yields of purified DNA, free from inhibitors that may affect downstream reactions such as genotyping assays, CE and next-generation sequencing, and high resolution melting analysis. The 96-prep and 5x 96-prep kits are designed for higher throughput and automat...
Catalog: 4413022
Size: 5 x 96 preps

NorthernMax®-Gly 10X Gel Prep/Running Buffer

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Northern Blotting Kits & Reagents

For the preparation and running of agarose gels. For use with RNA denatured by glyoxal treatment; one bottle containing 1 L is provided. Ambion® NorthernMax® reagents are the same components included in the NorthernMax® Ultrasensitive Northern Blotting Kits but in larger sizes. These reagents are supplied ready to use and are certified RNase-free. Comprehensive instructions are included.
Catalog: AM8678
Size: 1 L

Zero Blunt® TOPO® PCR Cloning Kit (with pCR®-Blunt II-TOPO®) with One Shot® TOP10 Chemically Competent E. coli and PureLink™ Quick Plasmid Miniprep Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > PCR Cloning Vectors

Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR®-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase (1). The pCR®-Blunt II-TOPO® Vector also features: • ccdB gene for positive selection (2) • Eco R I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli • SP6 promoter/primer site for...
Catalog: K2800-02
Size: 20 preps

Site Prep and Safety Guide, Sequence Detection System 7900HT

Category: Products & Services > Literature > Manuals > User Guides, Manuals & Bulletins

This is the Site Prep and Safety Guide for the 7900HT Sequence Detection System. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4317595
Size: 1 unit

Covaris® M220 microTUBE Prep Station

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Sequencing & Genetic Analyzer Instrument Accessories

The Covaris® M220 microTUBE Prep Station enables the easy loading of sample into snap-cap and screw-cap microTUBEs. Each package contains 1 preparation station.
Catalog: 4482270
Size: 1 each

Covaris® M220 miniTUBE Prep Station & Stand

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Sequencing & Genetic Analyzer Instrument Accessories

The Covaris® M220 miniTUBE Prep Station enables the easy loading of sample into clear, blue, or red miniTUBEs. A tilted stand is included that has been designed to help recover the entire sample from the miniTUBE. Each package contains 1 preparation station.
Catalog: 4482267
Size: 1 each

Corvaris™ S220 System

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing

The Covaris™ S220 System is a required sample preparation instrument for use in the SOLiD™ System workflow. The instrument is an essential part of the emulsion PCR process used to prepare the beads for emulsion PCR. The Covaris™ System is used to shear DNA into 60 bp fragments for fragment library preparation. The Covaris™ S220 System includes a Covaris™ S220 instrument, laptop, and a recirculating chiller
Catalog: 4465653
Size: 1 instrument

Covaris® M220 Focused-ultrasonicator™

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing

The Covaris® M220 Focused-ultrasonicator™ is designed for next generation sequencing applications that require high-quality DNA fragmentation for library preparation. The Covaris® M220 efficiently focuses the minimum amount of energy required directly into a sample, enabling precise control of the hydrodynamic shearing forces that fragment nucleic acids to the selected size. The process is isothermal, so fragmentation is unbiased, the sample is undamaged, and yields are high. The Covaris® focused-ultrasonic technology is considered a DNA shearing standard in many labs worldwide. The Covaris® M...
Catalog: 4482277
Size: 1 instrument

Ion Chip™ Minifuge, 120 V

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing

The personal-sized Ion Chip™ MiniFuge is designed and calibrated specifically for centrifugation of Ion PGM™/Ion Proton™ chips, enabling effective and efficient sample loading. The MiniFuge features a compact design with a small footprint. The cylindrical shape of the Ion Chip™ Minifuge allows it to accommodate both the Ion PGM™ and the larger Ion Proton™ chips. The unit quickly accelerates to maximum speed and stops quickly. • Tested and validated by Ion Torrent • Spins Ion PGM™ and Ion Proton™ chips, enabling effective and efficient sample loading • Small footprint, compact design The Ion Ch...
Catalog: 4479672
Size: 1 each

Ion Chip™ Minifuge, 230 V

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing

The personal-sized Ion Chip™ MiniFuge is designed and calibrated specifically for centrifugation of Ion PGM™/Ion Proton™ chips, enabling effective and efficient sample loading. The MiniFuge features a compact design with a small footprint. The cylindrical shape of the Ion Chip™ Minifuge allows it to accommodate both the Ion PGM™ and the larger Ion Proton™ chips. The unit quickly accelerates to maximum speed and stops quickly. • Tested and validated by Ion Torrent • Spins Ion PGM™ and Ion Proton™ chips, enabling effective and efficient sample loading • Small footprint, compact design The Ion Ch...
Catalog: 4479673
Size: 1 each

Ion Chef™ Instrument

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing

The Ion Chef™ System* is the next generation of workflow simplification products for the Ion Proton™ and Ion PGM™ systems. The Ion Chef™ System provides automated, high-throughput template preparation and reproducible chip loading for users at any experience level. With typically less than 15 minutes of hands-on time, an intuitive touch-screen interface, and significantly higher productivity than the Ion OneTouch™ 2 System, the Ion Chef™ System makes the Ion Torrent™ sequencing workflow accessible for every lab. The Ion Chef™ System: • Simplifies the workflow • Minimizes sources of user-introd...
Catalog: 4484177
Size: 1 system