phenol red solution preparation

Opti-MEM® Reduced Serum Medium, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Opti-MEM® I

Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 11058-021
Size: 500 ml

DMEM, Low Glucose, Pyruvate, no Glutamine, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • Low Glucose • L-glutamine • Sodium Pyruvate • Phenol Red • HEPES
Catalog: 11054-001
Size: 1 L

Medium 199, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Media 199

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. Life Technologies offers a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This M199 is modified as follows: With Without • L-glutamine • Phenol Red • HEPES
Catalog: 11043-023
Size: 500 ml

IMDM, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Iscove's (IMDM)

Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. Life Technologies offers a variety of Gibco® IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This IMDM is modified as follows: With Without • L-glutamine • Phenol Red • α-thioglycerol • 2-mercaptoethanol
Catalog: 21056-023
Size: 500 ml

0.5% Trypsin-EDTA (10X), no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin

Gibco® Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing This Trypsin is modified as follows: With Without • EDTA • Phenol Red
Catalog: 15400-054
Size: 100 ml

TrypLE™ Express (1X), Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > TrypLE™

TrypLE™ Express is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. Life Technologies offers a variety of TrypLE ™ formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Express is: Gentle on cells —maintains cell health for reproducible results Room-temperature stable —convenient to use Easy to use —substitutes directly into existing protocols, with no inactivation required Animal origin fre...
Catalog: 12605-010
Size: 100 ml

0.05% Trypsin-EDTA (1X), Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin

Gibco® Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing This Trypsin is modified as follows: With • EDTA • Phenol Red
Catalog: 25300-120
Size: 20 x 100 ml

MEM, 2X Temin's, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Modified Eagle Medium (MEM)

Temin's Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • L-glutamine • HEPES • Earle's salts • Phenol Red • Sodium Bicarbonate
Catalog: 11935-046
Size: 500 ml

DMEM, High Glucose, no Glutamine, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES
Catalog: 31053-028
Size: 500 ml

SYTO® 60 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains

The cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.
Catalog: S11342
Size: 250 µl

MEM Vitamin Solution (100X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Vitamin Solutions

Gibco® MEM Vitamin Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Vitamin Solution is formulated to contain 100x the vitamins found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Vitamin Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001...
Catalog: 11120-052
Size: 100 ml

MEM Amino Acids Solution, 50X

Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Amino Acid Solutions

Gibco® MEM Amino Acids Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Amino Acids Solution is formulated to contain 50X the essential amino acids (except L-glutamine) found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Amino Acids Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufactur...
Catalog: 11130-051
Size: 100 ml

Texas Red®-X Goat Anti-Rabbit IgG (H+L)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

The Texas Red-X goat anti—rabbit IgG is labeled with our bright, photostable, red-fluorescent dye (which contains an additional seven-atom aminohexanoyl spacer ("X") between the fluorophore and the antibody). The antibody is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize crossreactivity, it has been adsorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum.
Catalog: T-6391
Size: 0.5 ml

ChromaTide® Texas Red®-12-dUTP

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > Fluorescently Labeled Nucleotides

Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis. ChromaTide® Labeled Nucleotides Specifications: • Ex/Em of dye: Texas Red®-12-dUTP (595/615 nm) • Length...
Catalog: C-7631
Size: 25 µl

Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit - 500 assays

Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Metabolic Assay Kits

The Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit contains a sensitive, one-step assay that uses the Amplex® Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) to detect hydrogen peroxide (H 2 O 2 ) or peroxidase activity. The Amplex® Red reagent, in combination with horseradish peroxidase (HRP), has been used to detect H 2 O 2 released from biological samples, including cells, or generated in enzyme-coupled reactions. Furthermore, Amplex® Red reagent can be used as an ultrasensitive assay for peroxidase activity when H 2 O 2 is in excess. In the presence of peroxidase, the Amplex® Red reag...
Catalog: A22188
Size: 1 kit

Texas Red®-X Phalloidin

Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents

Texas Red®-X phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Texas Red®-X dye. Selectively stains F-actin Outstanding fluorescence performance Excitation/Emission: 591/608 nm Superior to antibody staining Optimal for fixed and permeabilized samples Get Superior Results in Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually i...
Catalog: T7471
Size: 300 units

Sodium Pyruvate 100 mM Solution

Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Sodium Pyruvate

Sodium Pyruvate is commonly added to cell culture medium as an energy source in addition to glucose. Since the cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not necessary to supplement all cultures. However, if the cells have been grown in medium supplemented with sodium pyruvate, we recommend the supplementation is continued as the cells become accustomed to this addition and their growth will lag without it. Gibco® Sodium Pyruvate, 100 mM Solution is formulated as 11 g per liter of water. The final concentration of sodium pyruvate used in most cell...
Catalog: 11360-070
Size: 100 ml

Lectin PHA-L From Phaseolus vulgaris (red kidney bean), Alexa Fluor® 647 Conjugate

Category: Products & Services > Labeling & Detection Products > BioConjugates > Lectins

Lectin phytohemagglutinin-L (PHA-L) is strongly inhibited by N-acetylglucosamine β(1-2) mannopyranosyl residues.[ALTREF PN29369] This lectin has been shown to agglutinate leukocytes and induce mitogenic activity in T-lymphocytes[ALTREF PN29369,PN29368] and has also been used as an anterograde tracer for the study of neuronal pathways.[ALTREF PN20284]
Catalog: L32457
Size: 1 mg

HCS LipidTOX™ Red Neutral Lipid Stain, for cellular imaging

Category: Products & Services > Kits & Assays > Cell Analysis Kits > HCS Detection Kits

The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid sta...
Catalog: H34476
Size: 1 each