Peptide Products
Matched and validated lipid and peptide substrates are available to complement the Adapta® Universal Kinase Assay. The assays developed with these reagents allow for primary or secondary screening of kinase inhibitors.
Catalog: PV5707
Size: 1 ml
Size: 1 ml
Platinum® Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA Polymerase from Thermococcus species KOD with proofreading (3´a5´ exonuclease) activity. Platinum® antibody technology provides a simple, automatic hot-start method that improves PCR specificity (2,3) (Figure 1). PCR x Enhancer Solution is included for higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of Platinum® Pfx DNA Polymera...
Catalog: 11708-013
Size: 100 reactions
Size: 100 reactions
Ambion® TURBO DNA- free ™ contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. The kit includes sufficient reagents for 50 rxns. Features of Ambion® TURBO DNA- free ™ include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can plague RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The Best Method for Genomic DNA Removal Prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNa...
Catalog: AM1907
Size: 50 reactions
Size: 50 reactions
PKN1 (PRK1) belongs to the protein kinase C superfamily. This kinase is activated by the Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can also be activated by phospholipids.
Catalog: PV3790
Size: 10 µg
Size: 10 µg
PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half (Figure 1). PCR SuperMix contains recombinant Taq DNA polymerase for routine PCR of fragments up to 5kb.
Catalog: 10572-014
Size: 100 reactions
Size: 100 reactions
S1 Nuclease is a single-strand-specific endonuclease that hydrolyzes single-stranded RNA or DNA into 5´ mononucleotides. The enzyme will hydrolyze single-stranded regions in duplex DNA such as loops and gaps. S1 Nuclease is stable at 65°C. Applications: Nuclease mapping techniques (1,2). Removal of single-stranded regions from double-stranded DNA (3). Exo III-ordered sequencing (4). Source: Isolated from Aspergillus oryza e. Performance and Quality Testing: Double-strand-specific deoxyribonuclease and phosphatase assays. Unit Definition: One unit hydrolyzes 1 µg of denatured DNA to acid-solubl...
Catalog: 18001-016
Size: 20,000 units
Size: 20,000 units
5´-CCC↓GGG-3´ 3´-GGG↑CCC-5´ Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns. Concentration 4-12 U/ µL Contents Sma I 10X Buffer T (1mL) 10X Loading Buffer (1mL) Reaction Temperature 30°C Reaction Mixture Sma I 1 µL 10X Buffer T 2 µL 0.10% BSA 2 µL Substrate DNA Heat inactivation Enzyme inactivated by heating at 65°C for 20 minutes....
Catalog: 15228-018
Size: 1,000 units
Size: 1,000 units
BMPR1A is a Type I member of the TGF beta receptor superfamily of transmembrane ser⁄thr kinases which phosphorylates intracellular SMADs in response to bone morphogenic proteins.
Catalog: PV6038
Size: 10 µg
Size: 10 µg
Applied Biosystems® MultiScribe™ Reverse Transcriptase is a recombinant Moloney murine leukemia virus (rMoMuLV) reverse transcriptase. MultiScribe™ Reverse Transcriptase is similar to MuLV reverse transcriptase but differs in its recommended usage. MultiScribe™ Reverse Transcriptase is a recombinant RNA-dependent DNA polymerase that uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand. 5000U supplied in 100 µl (50U/ µl). • Compatible with one-step and two-step RT-PCR reactions, which provides flexibility in assay set-up • Individual...
Catalog: 4311235
Size: 100 µl
Size: 100 µl
DNA End Repair Mix offers an easy, one step process for creating blunt ends in sheared, sonicated, or restriction-cut DNA and PCR fragments for efficient ligation into blunt end cloning vectors. DNA End Repair Mix allows you to: Generate blunt ends in DNA and PCR fragments (A-tail) Repair DNA with 5′ or 3′ overhangs in 30 minutes at 37°C Heat inactivate DNA End Repair Mix in 10 minutes at 75°C A Simple Way to Make Blunt Ends With DNA End Repair Mix, DNA with 5′ or 3′ overhangs is converted to 5′ phosphorylated blunt end DNA in 30 minutes at 37°C. Once DNA has been repaired, the enzymes in DNA ...
Catalog: A14321
Size: 20 reactions
Size: 20 reactions
MAP3K14 (NIK) is structurally related to MAP3Ks. MAP3K14 phosphorylates and activates CHUK (IKKalpha) and IKBKB (IKK beta), which in turn phosphorylate IkB-alpha, releasing NF-KappaB leading to up-regulation of inflammatory response genes.
Catalog: PV4902
Size: 10 µg
Size: 10 µg
Applied Biosystem® AmpErase® Uracil N-glycosylase (UNG), part of the GeneAmp® PCR Carry-over Prevention Kit, is a 26 kDa ultrapure, recombinant enzyme encoded by the E. coli uracil N-glycosylase gene, which has been inserted into an E. coli host to direct the expression of the wild type form of the enzyme. The GeneAmp® PCR Carry-over Prevention Kit provides reagents for a simple yet powerful method of ensuring that PCR products cannot be reamplified in subsequent PCR amplifications, thereby preventing false positive results. • Enzymatic method stops PCR carryover contamination, which prevents ...
Catalog: N8080096
Size: 100 units
Size: 100 units
Ambion® RNase Cocktail™ is a mixture of two highly purified ribonucleases, RNase A (500 U/mL) and RNase T1 (20,000 U/mL), and is free of DNase and nicking activities. Use RNase Cocktail™ for all situations where it is desirable to degrade RNA, i.e. plasmid minipreps and ribonuclease protection assays. Supplied in five tubes containing 1 mL each. Digestion of RNA with RNase A alone leaves fragments of RNA that are large enough to be visible on agarose gels and precipitate in ethanol. RNase A cuts after C and U residues, and RNase T1 cuts after G residues. Consequently, the mixture of both enzym...
Catalog: AM2288
Size: 5 tubes
Size: 5 tubes
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins. Proteinase K Remains Active: • Over a wide pH range (optimal activity between 6.5 and 9.5) • Under denaturing conditions (e.g. the presence of SDS or urea) • In the presence of metal chelating agents (e.g. EDTA) • At comparatively high temperatures (optimum digestion temperature is 65°C) Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization. Performance and Quality Testing: End...
Catalog: AM2546
Size: 1.25 ml
Size: 1.25 ml
Application/s In the isolation of DNA for RNA-free DNA. Concentration 20 mg RNase A/ml in 50 mM Tris-HCl (pH 8.0), 10 mM EDTA Specification/s An essentially protease free, freeze dried powder with an activity of not less than 70 U/mg material Source of product Bovine pancreas EC No. 3.1.27.5
Catalog: 12091-039
Size: 25 ml
Size: 25 ml
The PureLink® DNase Set provides rapid and efficient removal of DNA from RNA that has been purified using PureLink® RNA kits. The PureLink® DNase Set is: • RNase-free for use in RNA purification • Optimized for on-column digestion • Lyophilized for stability The RNase-free DNase set comes lyophilized and is optimized for on-column digestion of DNA using PureLink® protocols. The degree of DNA digestion performed by PureLink® DNase is optional for most PureLink® RNA kits, due to the efficient removal of the majority of DNA by the PureLink® technology; however, some downstream applications that a...
Catalog: 12185010
Size: 50 preps
Size: 50 preps
Ambion® TURBO DNA- free ™ contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. The kit includes sufficient reagents for 50 rxns. Features of Ambion® TURBO DNA- free ™ include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can plague RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The Best Method for Genomic DNA Removal Prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNa...
Catalog: AM1907M
Size: 50 rxns w⁄ Manual
Size: 50 rxns w⁄ Manual
DNase I, Amplification Grade digests single- and double-stranded DNA to oligodexyribonuleotides. The specific activity is >10,000 units/mg. DNase I, Amplification Grade is purified and tested for non-detectable levels of RNase contamination. Absence of RNase is tested by performing a Ribonuclease assay with RNA ladder. Application: Removing DNA from RNA (1) and protein preparations. Source: Purified from bovine pancreas. Performance and Quality Testing: Ribonuclease assay with RNA ladder; ability to digest single-stranded and double-stranded DNA to oligonucleotides determined. Unit Definition:...
Catalog: 18068-015
Size: 100 units
Size: 100 units
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins. Proteinase K Remains Active: • Over a wide pH range (optimal activity between 6.5 and 9.5) • Under denaturing conditions (e.g. the presence of SDS or urea) • In the presence of metal chelating agents (e.g. EDTA) • At comparatively high temperatures (optimum digestion temperature is 65°C) Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization. Performance and Quality Testing: End...
Catalog: AM2548
Size: 5 x 1.25 ml
Size: 5 x 1.25 ml
MAPK9 (JNK2) is a serine⁄threonine kinase which plays an important role in the MAPK pathway.
Catalog: PV4018
Size: 1 mg
Size: 1 mg
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