Pcr Standard
The mir Vana™ qRT-PCR Detection Kit has been optimized to amplify specific small RNA using qRT-PCR primer sets (not included), enabling accurate quantitation of miRNA expression levels. The novel detection system can be used to characterize miRNA expression via traditional endpoint RT-PCR or real-time RT-PCR using SYBR® Green I. • Quantitative results in less than 3 hours • Detection from as little as 25 pg of total RNA • Suitable for either real-time or end-point RT-PCR • Ideal for validating expression profiling data and high-throughput applications. MicroRNAs (miRNAs) are evolutionarily con...
Catalog: AM1558
Size: 200 reactions
Size: 200 reactions
Perform up to 2,000 PCR reactions with Applied Biosystems® TaqMan® PCR Core Reagent kit, 10-Pack. Applied Biosystems® TaqMan® PCR Core Reagents, 10-pack provides the individual components needed to perform up to 2,000 PCR reactions (50 µl each). Features of the TaqMan® PCR Core Reagent Kit: • Separate components, packaged in 10 tubes, provide maximum flexibility in assay setup • AmpliTaq Gold® DNA polymerase, a chemically modified form of AmpliTaq® DNA Polymerase, improves specificity and yield • AmpErase® UNG protects against subsequent re-amplifications from PCR products containing dU to min...
Catalog: 402930
Size: 2000 reactions
Size: 2000 reactions
Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® re...
Catalog: 4326614
Size: 2000 reactions
Size: 2000 reactions
Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. • Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR • The optimized formulation contains highly purified AmpliTaq Gold® D...
Catalog: 4367659
Size: 200 reactions
Size: 200 reactions
Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® re...
Catalog: 4364341
Size: 400 reactions
Size: 400 reactions
Applied Biosystems® TaqMan® Universal PCR Master Mix is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® real-time PCR instru...
Catalog: 4304437
Size: 200 reactions
Size: 200 reactions
Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial. • Premixed components stored at 2–8°C significantly reduce assay setup time • SYBR® Green I dye detects double-stranded DNA, so specific probes are not required • AmpliTaq Gold® DNA polymerase minimizes nonspecific product formation to achieve superior performance • dUTP significant...
Catalog: 4309155
Size: 200 reactions
Size: 200 reactions
This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR® Green I, VIC®/JOE™, and ROX™ dyes on the StepOne™ Real-Time PCR System. Features of the StepOne™ Real-Time PCR System Spectral Calibration Kit include: • 48-well optical reaction plates preloaded with dyes (FAM™/SYBR® Green I, VIC®/JOE™, and ROX™) are provided for quick and easy calibration of the StepOne™ Real-Time PCR System • Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture • Includes the Passive Reference I dye standard for sig...
Catalog: 4371433
Size: 1 kit
Size: 1 kit
The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7300 and 7500 Real-Time PCR Systems. The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is a 96-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. T...
Catalog: 4350584
Size: 1 kit
Size: 1 kit
Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR® Green RNA-to-C T ™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes: • AmpliTaq® Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA • An additive that red...
Catalog: 4391178
Size: 40 reactions
Size: 40 reactions
Get precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems® TaqMan® Gene Expression Master Mix. TaqMan® Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan® Gene Expression Master Mix has been validated with TaqMan® Gene Express...
Catalog: 4369542
Size: 2000 reactions
Size: 2000 reactions
TaqMan® Universal Master Mix II is a convenient 2X mix for use in all real-time application including microRNA, gene expression and genotyping. TaqMan® Universal Master Mix II is a convenient 2X mix that includes: -AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure), a highly purified thermostable DNA polymerase, that is hot start-enabled for convenient reaction setup at room temperature -Available in two formats: with and without uracil-DNA glycosylase (UDG) and dUTP⁄dTTP mix to minimize carryover PCR contamination -Optimized salt, dNTP, and buffer concentrations for reliable performance -Passive ...
Catalog: 4440043
Size: 40 reactions
Size: 40 reactions
This kit contains components needed to perform up to 1000 assays using TaqMan® probes. The kit employs AmpliTaq Gold® DNA polymerase for enhanced performance, 10X TaqMan Buffer A, and magnesium chloride. The components are provided in bulk and enable flexibility in assay setup. Separate Components Provide Flexibility in Assay Set-Up AmpliTaq Gold® DNA polymerase increses yield and gives a more robust 5' nuclease assay than AmpliTaq® DNA polymerase. Passive Reference I in the 10X TaqMan® Buffer A normalizes signal in all TaqMan® Assay reactions and corrects inter-well signal variation caused by...
Catalog: 4304441
Size: 1000 reactions
Size: 1000 reactions
The Applied Biosystems® MicroAmp® Optical 384-Well Reaction Plate with barcode is designed to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification. This plate, constructed from a single rigid piece of polypropylene in a 384-well format, is designed to work with Applied Biosystems® 384-well Real-Time PCR Systems and Thermal Cyclers. • Frosted to minimize interfering fluorescence from wells of cycling block • Screened to eliminate auto-fluorescent plates • Unique design provides a barrier to ambient air to help insure well-to-well temperature uniformity • E...
Catalog: 4326270
Size: 500 plates
Size: 500 plates
The Applied Biosystems® MicroAmp® Optical 384-Well Reaction Plate is designed to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification. This plate, constructed from a single rigid piece of polypropylene in a 384-well format, is designed to work with Applied Biosystems® 384-well Real-Time PCR Systems and Thermal Cyclers. • Frosted to minimize interfering fluorescence from wells of cycling block • Screened to eliminate auto-fluorescent plates • Unique design provides a barrier to ambient air to help insure well-to-well temperature uniformity • Each reaction ...
Catalog: 4309849
Size: 50 plates
Size: 50 plates
The Applied Biosystems® MicroAmp® Optical 96-Well Reaction Plate is optimized to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification. This plate, constructed from a single rigid piece of polypropylene in a ninety-six well format is compatible with Applied Biosystems® 96-Well Real-Time PCR Systems and Thermal Cyclers. • Frosted to minimize interfering fluorescence from wells of cycling block • Screened to eliminate auto-fluorescent plates • Unique design provides a barrier to ambient air to help insure well-to-well temperature uniformity • Each reaction p...
Catalog: 4326659
Size: 500 plates
Size: 500 plates
The Applied Biosystems® MicroAmp® Optical 96-Well Reaction Plate is optimized to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification. This plate, constructed from a single rigid piece of polypropylene in a ninety-six well format is compatible with Applied Biosystems® 96-Well Real-Time PCR Systems and Thermal Cyclers. • Frosted to minimize interfering fluorescence from wells of cycling block in real-time PCR applications • Screened to eliminate auto-fluorescent plates • Unique design provides a barrier to ambient air to help insure well-to-well temperatur...
Catalog: N8010560
Size: 10 plates
Size: 10 plates
The TaqMan® GTXpress™ Master Mix delivers accurate genotyping results with robust performance in less than 50 min. Applied Biosystems® TaqMan® GTXpress™ Master Mix (2X) combines AmpliTaq® Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format. The 1 mL pack includes reagents sufficient to perform 400 PCR reactions (5 µL/reaction). • Compatible with both Fast and Standard mode thermal cycling conditions • Pre-mixed components stored at 2°–8°C significantly reduce assay setup time • Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays •...
Catalog: 4403311
Size: 400 reactions
Size: 400 reactions
The Applied Biosystems® TaqMan® Array Human Cardiomyocyte Differentiation by BMP Receptors 96-well Plate contains 28 assays to Cardiomyocyte Differentiation by BMP Receptors associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene s...
Catalog: 4414115
Size: 1 standard plate
Size: 1 standard plate
The Applied Biosystems® TaqMan® Array Mouse Immune Response 96-well Plate contains 92 assays to immune response associated genes and 4 assays to candidate endogenous control genes. The panel of assays in this plate targets genes from immune system functions that fall into 9 classes: Cell Surface Receptors; Stress Response; Oxidoreductases; Proteases; Transcription Factors; Signal Transduction; Cytokines and Cytokine Receptors; Chemokines and Chemokine Receptors; and Cell Cycle and Protein Kinases. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan...
Catalog: 4414079
Size: 1 standard plate
Size: 1 standard plate
Pcr Standard
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