Pcr Gene Expression
Get precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems® TaqMan® Gene Expression Master Mix. TaqMan® Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan® Gene Expression Master Mix has been validated with TaqMan® Gene Express...
Catalog: 4369542
Size: 2000 reactions
Size: 2000 reactions
Custom TaqMan® Gene Expression Assay, LG
Catalog: 4332079
Size: 2900 reactions
Size: 2900 reactions
Same as TaqVet Schmallenberg Virus - S Gene - Real-Time PCR Kit (SBVS/50) TaqMan® probes - RNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT en...
Catalog: SBVS50
Size: 50 tests
Size: 50 tests
Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® re...
Catalog: 4364341
Size: 400 reactions
Size: 400 reactions
Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® re...
Catalog: 4326614
Size: 2000 reactions
Size: 2000 reactions
The Primer Express® Software v3.0.1 allows you to design your own primers and probes using TaqMan® and SYBR® Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications. Developed specifically for use with our StepOne®, StepOnePlus®, 7300, 7500, 7500 Fast, 7900HT, ViiA™ 7, and QuantStudio™ real-time PCR systems, Primer Express® Software provides customized application-specific documents for absolute⁄relative quantitation and allelic discrimination. • Enables individual oligonucleotide design for real-time PCR applications. • Supports assays based o...
Catalog: 4363993
Size: 1 license
Size: 1 license
PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half (Figure 1). PCR SuperMix contains recombinant Taq DNA polymerase for routine PCR of fragments up to 5kb.
Catalog: 10572-014
Size: 100 reactions
Size: 100 reactions
The PichiaPink™ Expression Strain Set contains the 4 Pichia pastoris strains for use with the PichiaPink™ Expression System Kits. Each strain comes as a frozen stock in YPD media with 15% glycerol. All PichiaPink™ strains have the ade2 deletion. 3 strains have deletions of two protease genes, PEP4 and PRB1 . You can use the PichiaPink™ Expression Strain Set to refill your PichiaPink™ Yeast Expression System Kits. The PichiaPink™ Expression Strain Set includes the following Pichia strains: • PichiaPink™ Strain 1: ade2 • PichiaPink™ Strain 2: ade2 , pep4 • PichiaPink™ Strain 3: ade2 , prb1 • Pic...
Catalog: A11154
Size: 4 × 1ml
Size: 4 × 1ml
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Catalog: K6025-01
Size: 20 reactions
Size: 20 reactions
Applied Biosystems® GeneAmp®10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold® DNA Polymerase, resulting in highly specific, robust PCR amplification. When diluted 1:10, provides the optimal pH and ionic strength for PCR activation and amplification with AmpliTaq Gold® DNA Polymerase, ensuring maximum performance Available in several convenient sizes GeneAmp® 10X PCR Buffer II is both magnesium ion free and gelatin free. A separate MgCl 2 solution is provided. When used together, GeneAmp® 10X PCR Buffer II and MgCl 2 solution offer more versatility in...
Catalog: 4306898
Size: 6 × 1.5 ml
Size: 6 × 1.5 ml
Applied Biosystems® TaqMan® Universal PCR Master Mix is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® real-time PCR instru...
Catalog: 4304437
Size: 200 reactions
Size: 200 reactions
The pcDNA™3.1/V5-His TOPO® TA Expression Kit offers one-step cloning of Taq -amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1/V5-His-TOPO® vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants. In addition, the vector includes the following features: Strong CMV promoter for high-level, constitutive expression. C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody. C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating...
Catalog: K4800-40
Size: 40 reactions
Size: 40 reactions
Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. • Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR • The optimized formulation contains highly purified AmpliTaq Gold® D...
Catalog: 4367659
Size: 200 reactions
Size: 200 reactions
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K101-01
Size: 20 reactions
Size: 20 reactions
The ViiA™ 7 Real-Time PCR System combines all of the qPCR features you want in a single high performance instrument, so that you can optimize your research productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, the ViiA™ 7 System offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or fast-cycling 384- or 96-well plates and TaqMan® Array Micro Fluidic Cards and Reagents makes high-productivity real-time PCR accessible to any size l...
Catalog: 4453535
Size: 1 instrument
Size: 1 instrument
Need Results Fast Applied Biosystems® TaqMan® Fast Universal Master Mix (2x), No AmpErase® UNG delivers results in 40 minutes for 40 cycles of PCR in a 20 µL reaction volume using the Applied Biosystems® 7900HT and Fast 7500 Fast Real-Time PCR Systems. • Everything you need for TaqMan®-based qPCR amplification and detection in a convenient single-tube format. TaqMan® Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR • TaqMan® Fast Universal PCR Master Mix (2x), No AmpErase® UNG is compatible with TaqMan® Gene Expressio...
Catalog: 4366072
Size: 500 reactions
Size: 500 reactions
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 12562-013
Size: 5 transfections
Size: 5 transfections
This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR® Green I, VIC®/JOE™, and ROX™ dyes on the StepOne™ Real-Time PCR System. Features of the StepOne™ Real-Time PCR System Spectral Calibration Kit include: • 48-well optical reaction plates preloaded with dyes (FAM™/SYBR® Green I, VIC®/JOE™, and ROX™) are provided for quick and easy calibration of the StepOne™ Real-Time PCR System • Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture • Includes the Passive Reference I dye standard for sig...
Catalog: 4371433
Size: 1 kit
Size: 1 kit
The ViiA™ 7 Real-Time PCR System combines all of the qPCR features you want in a single high performance instrument, so that you can optimize your research productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, the ViiA™ 7 System offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or fast-cycling 384- or 96-well plates and TaqMan® Array Micro Fluidic Cards and Reagents makes high-productivity real-time PCR accessible to any size l...
Catalog: 4453537
Size: 1 instrument
Size: 1 instrument
The Champion™ pET SUMO Expression System produces the highest levels of soluble protein in E. coli . It utilizes a small ubiquitin-related modifier (SUMO) fusion, belonging to the growing family of ubiquitin-related proteins, to enhance the solubility of expressed fusion proteins. In contrast to ubiquitin, SUMO is involved in the stabilization and localization of proteins in vivo . After expression, the 11 kd SUMO moiety can be cleaved by the highly specific and active SUMO (ULP-1) protease at the carboxyl terminal, producing a native protein*. The Champion™ pET SUMO Protein and Peptide Expres...
Catalog: K300-01
Size: 20 reactions
Size: 20 reactions
Pcr Gene Expression
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