Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics
Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hydroscopicus . Hygromycin B acts by binding to the 80S subunit of the bacterial ribosome and inhibiting protein synthesis. Gibco® Hygromycin B is used as a bacterial selective antibiotic in the concentration range of 100 - 1000 µg / ml. This product is supplied as a 50 mg / ml solution. Resistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene ( hyg or hph ). Hygromycin B uses a different mode of action than Geneticin®, Blasticidin S, or Zeocin™, so it is excellent for use in dual-selec...
Catalog: 10687-010
Size: 20 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics
Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg / ml for bacteria, or 200 – 500 µg / ml for most mammalian cells. This product is supplied as a 50 mg / ml solution in...
Catalog: 10131-019
Size: 20 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics
Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg / ml for bacteria, or 200 – 500 µg / ml for most mammalian cells. This product is supplied as a 50 mg / ml solution in...
Catalog: 10131-027
Size: 100 ml
Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS
Catalog: 00-3000
Size: 12 × 1 L
Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS
Molecular biology grade, Ambion® PBS 10X, pH 7.4 solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminatin...
Catalog: AM9625
Size: 1 L
Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS
Catalog: 00-3002
Size: 100 tablets
Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS
Molecular biology grade, Ambion® PBS 10X, pH 7.4 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contamina...
Catalog: AM9624
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Balanced Salt Solutions > Phosphate-Buffered Salines
Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Life Technologies offers a variety of Gibco® PBS formulations for a range of cell culture applications. Find the right formulation using the media selector tool . This PBS is manufactured as follows: Without • Calcium • Magnesium • Phenol Red
Catalog: 10010-023
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Balanced Salt Solutions > Phosphate-Buffered Salines
Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Life Technologies offers a variety of Gibco® PBS formulations for a range of cell culture applications. Find the right formulation using the media selector tool . This PBS is manufactured as follows: Without • Calcium • Magnesium • Phenol Red
Catalog: 20012-068
Size: 10 x 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Cell Dissociation Buffers
Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Phosphate Buffered Saline (PBS). Gibco® Cell Dissociation Buffer is suitable for gentle dissociation of mammalian cells for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines such as HeLa and NIH 3T3, but is not recommended for routine passage of strongly adherent cells. The dis...
Catalog: 13151-014
Size: 100 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Vitamin Solutions
Gibco® MEM Vitamin Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Vitamin Solution is formulated to contain 100x the vitamins found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Vitamin Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001...
Catalog: 11120-052
Size: 100 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Amino Acid Solutions
Gibco® MEM Amino Acids Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Amino Acids Solution is formulated to contain 50X the essential amino acids (except L-glutamine) found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Amino Acids Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufactur...
Catalog: 11130-051
Size: 100 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Sodium Pyruvate
Sodium Pyruvate is commonly added to cell culture medium as an energy source in addition to glucose. Since the cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not necessary to supplement all cultures. However, if the cells have been grown in medium supplemented with sodium pyruvate, we recommend the supplementation is continued as the cells become accustomed to this addition and their growth will lag without it. Gibco® Sodium Pyruvate, 100 mM Solution is formulated as 11 g per liter of water. The final concentration of sodium pyruvate used in most cell...
Catalog: 11360-070
Size: 100 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > BSA & Albumin Supplements
7.5 g/100 ml Prepared with Bovine Serum Albumin Fraction V in phosphate-buffered saline.
Catalog: 15260-037
Size: 100 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Insulin, Transferrin, & Iron Supplements
Gibco® Insulin, Transferrin, Selenium, Sodium Pyruvate Solution (ITS -A) is used as a basal medium supplement in order to reduce the amount of Fetal Bovine Serum (FBS) used to culture the cells. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G is now routinely used with other basal media to support a wide variety of cell types in the presence of 2-4% FBS. Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and it may also help to reduce toxic levels of oxyge...
Catalog: 51300-044
Size: 10 ml